Temperature and metabolic compensation mechanisms in a circadian clock system

项目总结/摘要 生物钟是细胞内的酶系统，其提供生物化学反应。 当地时间的代表性，对不同国家的健康产生深远影响 有机体与大多数酶不同，生物钟需要对一个范围不敏感 生理温度和细胞能量水平， 可靠地预测黎明和黄昏。然而，昼夜节律的机制 任何生物体中的生物钟都实现了对温度的不敏感性（即，温度 补偿）和细胞能量水平（即，代谢补偿）， 明白因此，LiWang实验室未来五年的总体愿景是， 阐明温度补偿和代谢的机制 补偿蓝藻的生物钟。在过去的20年里，我们 在蓝藻生物钟的机制上有了许多有影响力的发现 并在此过程中开发了创新的方法和工具。因此，我们非常 有能力成功地填补知识领域的关键空白， 生物计时这里提出的工作的一个主要预期成果是 详细的因果模型，将时钟蛋白的行为和相互作用联系起来， 温度和代谢补偿表型。

Project Summary/Abstract Circadian clocks are intracellular enzymatic systems that provide a biochemical representation of local time with profound consequences to health across diverse organisms. Unlike most enzymes, biological clocks need to be insensitive to a range of physiological temperatures and cellular energy levels so that organisms can anticipate dawn and dusk reliably. However, the mechanisms by which circadian clocks in any organism achieve insensitivity to temperature (i.e., temperature compensation) and cellular energy levels (i.e., metabolic compensation) are far from understood. Therefore, the overall vision of the LiWang lab for the next five years is to elucidate the mechanisms of temperature compensation and metabolic compensation in the circadian clock of cyanobacteria. Over the past 20 years, we have made many impactful discoveries on mechanism of the cyanobacterial clock and developed innovative methodologies and tools along the way. Thus, we are very well positioned to succeed at filling critical gaps in knowledge in the field of biological timekeeping. A major expected outcome of the work proposed here is a detailed cause-and-effect model linking clock protein behavior and interactions to temperature and metabolic compensation phenotypes in vivo.