Temperature and metabolic compensation mechanisms in a circadian clock system

项目摘要/摘要 生物钟是细胞内的酶系统，它提供了一种生化 代表当地时间，对不同地区的健康产生深远影响 有机体。与大多数酶不同，生物钟需要对某一范围不敏感。 生理温度和细胞能量水平，这样生物体可以 可靠地预测黎明和黄昏。然而，昼夜节律的机制 任何生物体中的时钟都对温度(即温度)不敏感 补偿)和细胞能量水平(即新陈代谢补偿)远远不是 明白了。因此，力王实验室未来五年的总体愿景是 阐明温度补偿和代谢的机制 蓝藻生物钟的补偿。在过去的20年里，我们 在蓝藻生物钟的机制上有了许多有影响力的发现 并在此过程中开发了创新的方法和工具。因此，我们非常 处于有利地位，能够成功填补该领域知识的关键空白 生物计时。这里提出的工作的一个主要预期成果是 详细的因果模型将时钟蛋白的行为和相互作用联系起来 体内温度和代谢补偿表型。

Project Summary/Abstract Circadian clocks are intracellular enzymatic systems that provide a biochemical representation of local time with profound consequences to health across diverse organisms. Unlike most enzymes, biological clocks need to be insensitive to a range of physiological temperatures and cellular energy levels so that organisms can anticipate dawn and dusk reliably. However, the mechanisms by which circadian clocks in any organism achieve insensitivity to temperature (i.e., temperature compensation) and cellular energy levels (i.e., metabolic compensation) are far from understood. Therefore, the overall vision of the LiWang lab for the next five years is to elucidate the mechanisms of temperature compensation and metabolic compensation in the circadian clock of cyanobacteria. Over the past 20 years, we have made many impactful discoveries on mechanism of the cyanobacterial clock and developed innovative methodologies and tools along the way. Thus, we are very well positioned to succeed at filling critical gaps in knowledge in the field of biological timekeeping. A major expected outcome of the work proposed here is a detailed cause-and-effect model linking clock protein behavior and interactions to temperature and metabolic compensation phenotypes in vivo.