Human mast cell and tissue acquisition core

摘要 人类肥大细胞和组织获取核心B的目标是集中和直接的：提供 所有三个项目都将使用的关键服务。核心B将为其中每一个提供明确定义的材料 确保协调一致的方法的项目，并将在出现问题时进行故障排除。 位于西北大学范伯格医学院，由 B核心的Bruce Bochner博士将获取、分类、存储和运送来自患有 慢性鼻窦炎、嗜酸性胃肠道疾病和特应性皮炎 位于西北大学的医生和科学家主要用于项目3。第二项重要任务是 核心B是从废弃的人皮肤等组织中产生丰富的成熟人类肥大细胞， 以及西北纪念馆的人大肠和肺切除标本 用于体外实验的医院。核心B的第三个重点是协助和直接生成 通过将CD34+人细胞移植到三联细胞中的方法来人源化的肥大细胞小鼠 表达人IL-3、GM-CSF和SCF的转基因NSG-SGM3(NSGS)小鼠。最后，第四个 核心B的责任是维持重要的人类和小鼠肥大细胞系，并产生培养- 从表达人Siglecs的小鼠骨髓和从小鼠骨髓中提取的肥大细胞 移植的NSGS小鼠产生培养的人类肥大细胞，后两种细胞主要用于 项目1和2。简而言之，核心B将为主单元提供一个高效、标准化和集中化的来源， 人源化的肥大细胞小鼠和人类组织标本。在隔离和隔离方面的经验和专业知识 生成主单元将是项目1和项目2成功的关键，而去基元的可用性将是 从特征明确的患者和对照队列中识别的活检和丢弃的手术组织是 对项目3的目标至关重要。

ABSTRACT The goals of the human mast cell and tissue acquisition Core B are focused and straightforward: to provide key services that will be used by all three Projects. Core B will provide well-defined materials to each of these projects to ensure coordinated methods of approach, and will be available to troubleshoot if problems arise. Located at the Northwestern University Feinberg School of Medicine under the leadership and supervision of Dr. Bruce Bochner, Core B will procure, catalog, store and ship de-identified biopsy samples from subjects with chronic rhinosinusitis, eosinophilic gastrointestinal disorders and atopic dermatitis in collaboration with physicians and scientists located at Northwestern for use primarily by Project 3. A second important task for Core B is to generate enriched mature human mast cells derived from tissues such as discarded human skin, as well as samples of human large intestine and lung resection specimens from the Northwestern Memorial Hospital for use in in vitro experiments. The third focus of Core B is to assist with and directly generate “humanized” mast cell mice employing a method by which CD34+ human cells are transplanted into the triple transgenic NSG-SGM3 (NSGS) mice expressing human IL-3, GM-CSF and SCF. Finally, the fourth responsibility of Core B is to maintain important human and mouse mast cell lines, and to generate culture- derived mouse mast cells from bone marrow of mice expressing human Siglecs and from the bone marrow of the transplanted NSGS mice to generate culture-derived human mast cells, the latter two for use primarily by Projects 1 and 2. In short, Core B will provide an efficient, standardized and centralized source for mast cells, humanized mast cell mice, and human tissue specimens. The experience and expertise in isolating and generating mast cells will be critical to the success of Project 1 and Project 2, while the availability of de- identified biopsies and discarded surgical tissues from well-characterized cohorts of patients and controls are essential to the aims of Project 3.