Human mast cell and tissue acquisition core

人类肥大细胞和组织采集核心

• 批准号: 10331724
• 负责人: Bruce S Bochner
• 金额: $ 12.29万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-02-06 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10331724
• 关键词: Allergic Disease; Atopic Dermatitis; Biopsy; Biopsy Specimen; Bone Marrow; Bone Marrow Transplantation; CD34 gene; Catalogs; Cell Line; Cell Transplantation; Cells; Childhood; Collaborations; Endoscopic Biopsy; Ensure; Eosinophilic Gastrointestinal Disease; Excision; Generations; Goals; Granulocyte-Macrophage Colony-Stimulating Factor; Hospitals; Human; IL3 Gene; In Vitro; Large Intestine; Leadership; Letters; Ligands; Lung; Methods; Mucous Membrane; Mus; Nasal Polyps; Operative Surgical Procedures; Pathology; Patients; Physicians; Sampling; Scientist; Services; Ships; Sinus; Skin; Source; Specimen; Standardization; Supervision; Techniques; Tissues; Transgenic Mice; Transgenic Organisms; Universities; bone cell; chronic rhinosinusitis; cohort; cytokine; experience; experimental study; human tissue; mast cell; medical schools; sialic acid binding Ig-like lectin; success

ABSTRACT The goals of the human mast cell and tissue acquisition Core B are focused and straightforward: to provide key services that will be used by all three Projects. Core B will provide well-defined materials to each of these projects to ensure coordinated methods of approach, and will be available to troubleshoot if problems arise. Located at the Northwestern University Feinberg School of Medicine under the leadership and supervision of Dr. Bruce Bochner, Core B will procure, catalog, store and ship de-identified biopsy samples from subjects with chronic rhinosinusitis, eosinophilic gastrointestinal disorders and atopic dermatitis in collaboration with physicians and scientists located at Northwestern for use primarily by Project 3. A second important task for Core B is to generate enriched mature human mast cells derived from tissues such as discarded human skin, as well as samples of human large intestine and lung resection specimens from the Northwestern Memorial Hospital for use in in vitro experiments. The third focus of Core B is to assist with and directly generate “humanized” mast cell mice employing a method by which CD34+ human cells are transplanted into the triple transgenic NSG-SGM3 (NSGS) mice expressing human IL-3, GM-CSF and SCF. Finally, the fourth responsibility of Core B is to maintain important human and mouse mast cell lines, and to generate culture- derived mouse mast cells from bone marrow of mice expressing human Siglecs and from the bone marrow of the transplanted NSGS mice to generate culture-derived human mast cells, the latter two for use primarily by Projects 1 and 2. In short, Core B will provide an efficient, standardized and centralized source for mast cells, humanized mast cell mice, and human tissue specimens. The experience and expertise in isolating and generating mast cells will be critical to the success of Project 1 and Project 2, while the availability of de- identified biopsies and discarded surgical tissues from well-characterized cohorts of patients and controls are essential to the aims of Project 3.

摘要 人类肥大细胞和组织采集核心B的目标是集中和直接的：提供 这三个项目将使用的关键服务。核心B将为每个核心提供定义明确的材料， 项目，以确保协调方法的做法，并将提供故障排除，如果出现问题。 位于西北大学范伯格医学院的领导和监督下， 博士布鲁斯博克纳，核心B将从受试者中采购、分类、储存和运输去识别的活检样本， 慢性鼻窦炎、嗜酸性胃肠疾病和特应性皮炎， 西北大学的医生和科学家主要由项目3使用。第二项重要任务是 核心B是从组织如废弃的人皮肤产生富集的成熟人肥大细胞， 以及来自西北纪念馆的人类大肠和肺切除标本样本 医院用于体外实验。核心B的第三个重点是协助和直接生成 使用将CD 34+人细胞移植到三重细胞中的方法的“人源化”肥大细胞小鼠。 表达人IL-3、GM-CSF和SCF的转基因NSG-SGM 3（NSGS）小鼠。最后，第四个 核心B的职责是维持重要的人类和小鼠肥大细胞系，并产生培养物- 从表达人Siglec的小鼠的骨髓和从表达人Siglec的小鼠的骨髓衍生的小鼠肥大细胞 移植的NSGS小鼠产生培养衍生的人肥大细胞，后两者主要用于 项目1和2。简而言之，核心B将为肥大细胞提供高效、标准化和集中的来源， 人源化肥大细胞小鼠和人组织样本。在分离和 产生肥大细胞对项目1和项目2的成功至关重要，而去- 从充分表征的患者和对照组中鉴定的活检和丢弃的手术组织， 对项目3的目标至关重要。