Animal Core

动物核心

• 批准号: 10332261
• 负责人: Christopher Michael Peters
• 金额: $ 33.3万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-15 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10332261
• 关键词: Ablation; Affective; Anatomy; Animals; Behavior Control; Behavior assessment; Behavioral; Behavioral Assay; Biochemical; Biohazardous Substance; Brain; Breeding; Catheterization; Collaborations; Collection; Complex; Cryoultramicrotomy; Custom; DNA Sequence; Data; Data Analyses; Data Collection; Databases; Disease; Electrophysiology (science); Ensure; Enterobacteria phage P1 Cre recombinase; Equipment; Equipment and supply inventories; Generations; Genotype; Human Resources; IACUC; Immunohistochemistry; Individual; Injury; Label; Ligation; Maintenance; Mediating; Methods; Microscopy; Nervous system structure; Neurons; Operative Surgical Procedures; Outcome; Oxytocin; Pain; Perfusion; Plasma; Plasmids; Population; Preparation; Procedures; Protocols documentation; Quality Control; Rattus; Regulatory Affairs; Research; Safety; Sampling; Scheme; Sensory; Serotyping; Services; Ships; Source; Spinal Cord; Spinal nerve structure; Standardization; Statistical Data Interpretation; Structure; Structure of jugular vein; Supervision; Time; Tissue Banks; Tissue Harvesting; Tissue Sample; Tissues; Training; Transgenic Organisms; Vendor; Viral; Viral Packaging; Viral Vector; base; behavior test; brain tissue; data exchange; femoral artery; in vivo; microscopic imaging; novel; offspring; paraform; pharmacokinetics and pharmacodynamics; pre-clinical; preclinical study; programs; protein expression

The Animal Core will provide essential services and facilities to the preclinical projects in a standardized and efficient manner, utilizing shared facilities, equipment, and personnel in a coordinated fashion. The services to be provided to each preclinical project are divided into three specific aims. Dr. Christopher Peters will oversee the Animal Core and will meet with monthly or as needed with Drs. Boada, Martin, Romero-Sandoval to assure that the Core efficiently performs these three key functions. Dr. Peters will provide oversight for regulatory affairs within the Program regarding animal use including approval and compliance with protocols for the IACUC and Biohazard Safety Committee. Specific Aim 1 is to provide standardized in vivo surgical and behavioral procedures in animals utilized by preclinical projects. Both projects involving animals require animal procurement and identification, surgical preparation and behavioral assessment of sensory and affective pain related outcomes. Specific Aim 2 will provide standardized methods for collection of plasma and brain tissue and sample preparation for determination of oxytocin levels as part of PK/PD studies. We will standardize immunohistochemical approaches across preclinical projects for verification of viral mediated protein expression in discrete populations of neurons in brain or spinal cord tissue. These services include sacrifice and transcardial perfusion with paraformaldehyde, tissue harvest, cryoprotection and cryosectioning, immunohistochemistry, and fluorescent microscopy including data collection and analysis Specific Aim 3 will provide for generation of and maintenance of a novel transgenic oxytocin-cre rat colony. Dr. Romero-Sandoval will assist with colony management, breeding and genotyping to ensure sufficient animals are available in a timely manner for preclinical studies in Project 2. These essential functions will standardize and coordinate services and procedures throughout the Program and provide an efficient and costeffective structure for collaboration.

动物中心将为临床前项目提供必要的服务和设施， 标准化和有效的方式，利用共享设施，设备和人员， 协调的方式。为每个临床前项目提供的服务分为 三个具体目标。克里斯托弗·彼得斯博士将监督动物中心，并将会见 每月或根据需要与Boada、Martin、Romero-Sandoval博士联系，以确保核心 有效地执行这三个关键功能。Peters博士将负责监管 项目内有关动物使用的事务，包括批准和遵守 IACUC和生物危害安全委员会的协议。具体目标1是提供 标准化的动物体内手术和行为程序， 项目这两个涉及动物的项目都需要动物采购和鉴定， 感觉和情感疼痛相关结果的准备和行为评估。具体 目的2将为血浆和脑组织的采集和样本提供标准化的方法 作为PK/PD研究的一部分，用于测定催产素水平的制备。我们将标准化 临床前项目中用于验证病毒介导的 在脑或脊髓组织中的离散神经元群体中的蛋白质表达。这些 服务包括牺牲和经心灌注多聚甲醛，组织收获， 冷冻保护和冷冻切片、免疫组织化学和荧光显微镜检查，包括 数据收集和分析具体目标3将提供生成和维护 新型转基因催产素-cre大鼠群体。罗梅罗-桑多瓦尔博士会协助殖民地 管理、育种和基因分型，以确保及时提供足够的动物 项目2中临床前研究的方式。这些基本功能将标准化， 在整个计划中协调服务和程序，并提供有效和 具有成本效益的协作结构。