Animal Core

动物核心将为临床前项目提供必要的服务和设施 标准化、高效化，利用共享设施、设备、人员 协调时尚。为每个临床前项目提供的服务分为 三个具体目标。克里斯托弗·彼得斯 (Christopher Peters) 博士将监督动物核心，并将与 每月或根据需要与医生一起。博阿达、马丁、罗梅罗-桑多瓦尔确保核心 有效地执行这三个关键功能。彼得斯博士将负责监管 该计划内有关动物使用的事务，包括批准和遵守 IACUC 和生物危害安全委员会的协议。具体目标 1 是提供 临床前使用的动物体内标准化手术和行为程序 项目。这两个涉及动物的项目都需要动物采购和鉴定、手术 感觉和情感疼痛相关结果的准备和行为评估。具体 目标2将提供血浆和脑组织及样本采集的标准化方法 为测定催产素水平做准备，作为 PK/PD 研究的一部分。我们将标准化 跨临床前项目的免疫组织化学方法，用于验证病毒介导的 大脑或脊髓组织中离散神经元群中的蛋白质表达。这些 服务包括处死和多聚甲醛经心灌注、组织采集、 冷冻保护和冷冻切片、免疫组织化学和荧光显微镜，包括 数据收集和分析 具体目标 3 将提供生成和维护 新型转基因催产素-cre大鼠群体。罗梅罗-桑多瓦尔博士将协助殖民地 管理、育种和基因分型，以确保及时提供足够的动物 项目 2 中临床前研究的方式。这些基本功能将标准化和 协调整个计划的服务和程序，并提供高效和 具有成本效益的协作结构。

The Animal Core will provide essential services and facilities to the preclinical projects in a standardized and efficient manner, utilizing shared facilities, equipment, and personnel in a coordinated fashion. The services to be provided to each preclinical project are divided into three specific aims. Dr. Christopher Peters will oversee the Animal Core and will meet with monthly or as needed with Drs. Boada, Martin, Romero-Sandoval to assure that the Core efficiently performs these three key functions. Dr. Peters will provide oversight for regulatory affairs within the Program regarding animal use including approval and compliance with protocols for the IACUC and Biohazard Safety Committee. Specific Aim 1 is to provide standardized in vivo surgical and behavioral procedures in animals utilized by preclinical projects. Both projects involving animals require animal procurement and identification, surgical preparation and behavioral assessment of sensory and affective pain related outcomes. Specific Aim 2 will provide standardized methods for collection of plasma and brain tissue and sample preparation for determination of oxytocin levels as part of PK/PD studies. We will standardize immunohistochemical approaches across preclinical projects for verification of viral mediated protein expression in discrete populations of neurons in brain or spinal cord tissue. These services include sacrifice and transcardial perfusion with paraformaldehyde, tissue harvest, cryoprotection and cryosectioning, immunohistochemistry, and fluorescent microscopy including data collection and analysis Specific Aim 3 will provide for generation of and maintenance of a novel transgenic oxytocin-cre rat colony. Dr. Romero-Sandoval will assist with colony management, breeding and genotyping to ensure sufficient animals are available in a timely manner for preclinical studies in Project 2. These essential functions will standardize and coordinate services and procedures throughout the Program and provide an efficient and costeffective structure for collaboration.