Animal Core

动物核心将为临床前项目提供必要的服务和设施 标准化、高效地利用共享设施、设备和人员 协调一致的时尚。将为每个临床前项目提供的服务分为 三个具体目标。克里斯托弗·彼得斯博士将监督动物核心，并将会见 每月或根据需要与博达、马丁、罗梅罗-桑多瓦尔博士合作，以确保核心 有效地执行这三个关键功能。彼得斯博士将为监管机构提供监督 本计划内有关动物使用的事务，包括批准和遵守 IACUC和生物危害安全委员会的协议。具体目标1是提供 临床前使用的动物体内标准化手术和行为程序 项目。这两个涉及动物的项目都需要动物采购和鉴定，外科手术 感觉性和情感性疼痛相关结果的准备和行为评估。特定的 AIM 2将提供采集血浆、脑组织和样本的标准化方法 作为PK/PD研究的一部分，准备测定催产素水平。我们将标准化 用于验证病毒介导的临床前项目的免疫组织化学方法 脑或脊髓组织中离散神经元群体的蛋白质表达。这些 服务包括牺牲和多聚甲醛心脏灌注，组织采集， 冷冻保护和冷冻切片、免疫组织化学和荧光显微镜，包括 数据收集和分析具体目标3将规定生成和维护 新型转基因催产素-cre大鼠群体。罗梅罗-桑多瓦尔博士将协助殖民地 管理、饲养和基因分型，以确保及时提供足够的动物 项目2中临床前研究的方式。这些基本功能将标准化和 协调整个计划的服务和程序，并提供高效和 具有成本效益的协作结构。

The Animal Core will provide essential services and facilities to the preclinical projects in a standardized and efficient manner, utilizing shared facilities, equipment, and personnel in a coordinated fashion. The services to be provided to each preclinical project are divided into three specific aims. Dr. Christopher Peters will oversee the Animal Core and will meet with monthly or as needed with Drs. Boada, Martin, Romero-Sandoval to assure that the Core efficiently performs these three key functions. Dr. Peters will provide oversight for regulatory affairs within the Program regarding animal use including approval and compliance with protocols for the IACUC and Biohazard Safety Committee. Specific Aim 1 is to provide standardized in vivo surgical and behavioral procedures in animals utilized by preclinical projects. Both projects involving animals require animal procurement and identification, surgical preparation and behavioral assessment of sensory and affective pain related outcomes. Specific Aim 2 will provide standardized methods for collection of plasma and brain tissue and sample preparation for determination of oxytocin levels as part of PK/PD studies. We will standardize immunohistochemical approaches across preclinical projects for verification of viral mediated protein expression in discrete populations of neurons in brain or spinal cord tissue. These services include sacrifice and transcardial perfusion with paraformaldehyde, tissue harvest, cryoprotection and cryosectioning, immunohistochemistry, and fluorescent microscopy including data collection and analysis Specific Aim 3 will provide for generation of and maintenance of a novel transgenic oxytocin-cre rat colony. Dr. Romero-Sandoval will assist with colony management, breeding and genotyping to ensure sufficient animals are available in a timely manner for preclinical studies in Project 2. These essential functions will standardize and coordinate services and procedures throughout the Program and provide an efficient and costeffective structure for collaboration.