Determining the structural- and functional-level effects of diet-specific interventions on the gut microbiota of a diverse sample of Southern United States adults

确定特定饮食干预措施对美国南部成年人不同样本肠道微生物群的结构和功能水平影响

• 批准号: 10361024
• 负责人: Tiffany LaShaun Carson
• 金额: $ 38.46万
• 依托单位: H. LEE MOFFITT CANCER CTR & RES INST
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10361024
• 关键词: Acetates; Address; Adult; Affect; American; American diet; Bacteroides; Base Sequence; Bifidobacterium; Bile Acids; Biological Markers; Body Weight decreased; Butyrates; Cardiac health; Cardiovascular Diseases; Categories; Chronic Disease; Clinical; Clostridium; Colorectal Cancer; Consequentialism; Consumption; DASH diet; DNA Damage; Data; Deoxycholic Acid; Diet; Diet Modification; Dietary Fiber; Dietary Intervention; Dietary Practices; Environment; Fatty acid glycerol esters; Female; Fiber; Fusobacterium; Goals; Health; Health Benefit; Health Promotion; Health Status; Incidence; Individual; Inflammation; Interleukin-6; Intervention; Knowledge; Lactobacillus; Lead; Leukocyte L1 Antigen Complex; Link; Literature; Lithocholic Acid; Metabolic; Metagenomics; Microbe; Modification; Not Hispanic or Latino; Obesity; Outcome; Participant; Pathogenicity; Physicians; Physiological; Population; Population Heterogeneity; Porphyromonas; Prevention Research; Prevention approach; Preventive; Production; Propionates; Public Health; Race; Randomized; Recommendation; Research; Ribosomal RNA; Risk; Risk Factors; Ruminococcus; Sampling; Scientist; Structure; Taxonomy; Testing; Therapeutic; Time; United States; Volatile Fatty Acids; Work; arm; base; beta diversity; biobehavior; blood pressure reduction; cancer risk; colorectal cancer risk; colorectal cancer treatment; dietary; disorder risk; dysbiosis; efficacy testing; experimental study; feeding; fruits and vegetables; gut bacteria; gut microbiota; health disparity; improved; inflammatory disease of the intestine; inflammatory marker; innovation; liver inflammation; male; microbial; microbiome; mortality; nutrition; public health relevance; rRNA Genes; racial difference; racial disparity; racial diversity; recruit; sex; systemic inflammatory response

Racial disparities in colorectal cancer (CRC) incidence persist for blacks and whites. Given the previous work of our team documenting racial differences in the gut microbiota of blacks and whites and the evidence supporting the interaction between diet and the gut microbiota as a risk factor for colorectal cancer, the study of how various diets affect the structure and function of the gut microbiota across racial groups is warranted. Previous research has shown that the gut microbiota can be rapidly altered by changes in diet. For example, consumption of an extremely high fiber (>50 grams) diet has produced changes in the gut microbiota that are believed to reduce cancer risk. The Dietary Approaches to Stop Hypertension (DASH) diet, rich in fruits, vegetables, whole grains and low-fat dairy, is commonly recommended for heart health and has been shown to lower blood pressure and produce weight loss. However, to our knowledge, the effect of the DASH diet on the gut microbiota has not been studied. Because the DASH diet provides substantial fiber, we hypothesize that consumption of the DASH diet will lead to improvements in the gut microbiota of non-Hispanic black and white adults. In this proposal, we plan to investigate our hypothesis by recruiting a generally healthy sample of 112 black and white adults from Birmingham, AL to participate in a 28-day randomized, controlled feeding study. Participants will be randomized to receive either the DASH diet or a standard American diet. All meals will be provided by the study. Fecal samples will be collected at multiple time points before, during, and after the dietary intervention and will be analyzed using PCR to amplify the V4 region of the 16S rRNA gene and to sequence bases using the MiSeq platform. Sequenced data will then be analyzed using QIIME. We hypothesize that participants receiving the DASH diet will have a greater increase in alpha diversity and greater changes in abundances of CRC-associated microbes than participants receiving the standard American diet. We will also evaluate functional-level markers including bile acid and short chain fatty acid (SCFA) production and inflammatory markers. If our hypothesis is supported, we expect to see reduced production of secondary bile acids (e.g., deoxycholic acid), greater SCFA production (e.g, butyrate), and reduction in gut and systemic inflammation (e.g, calprotectin, IL-6) among participants receiving the DASH diet compared to the standard American diet. Our findings will provide preliminary evidence for the DASH diet as an approach for cultivating a healthier gut microbiota across racially diverse populations. These findings can impact clinical, translational, and population-level approaches for modification of the gut microbiota to reduce risk of chronic diseases like CRC.

黑人和白人结直肠癌 (CRC) 发病率的种族差异仍然存在。鉴于之前的 我们团队的工作记录了黑人和白人肠道微生物群的种族差异以及证据 支持饮食和肠道微生物群之间的相互作用作为结直肠癌的危险因素，该研究 不同的饮食如何影响不同种族群体肠道微生物群的结构和功能是有必要的。 先前的研究表明，肠道微生物群可以通过饮食的变化而迅速改变。例如， 摄入极高纤维（> 50 克）饮食已导致肠道微生物群发生变化，这些变化 据信可以降低癌症风险。预防高血压的饮食方法（DASH）饮食，富含水果， 蔬菜、全谷物和低脂乳制品通常被推荐用于心脏健康，并且已被证明可以 降低血压并减轻体重。然而，据我们所知，DASH 饮食对 尚未研究肠道微生物群。由于 DASH 饮食提供大量纤维，我们假设 食用 DASH 饮食将改善非西班牙裔黑人和白人的肠道微生物群 成年人。在本提案中，我们计划通过招募 112 名总体健康的样本来研究我们的假设。 来自阿拉巴马州伯明翰的黑人和白人成年人参加了一项为期 28 天的随机对照喂养研究。 参与者将被随机分配接受 DASH 饮食或标准美式饮食。所有餐食将 该研究提供。将在饮食之前、期间和之后的多个时间点收集粪便样本 干预，并将使用 PCR 进行分析，以扩增 16S rRNA 基因的 V4 区域并测序 使用 MiSeq 平台的基础。然后使用 QIIME 分析测序数据。我们假设 接受 DASH 饮食的参与者的 α 多样性将有更大的增加，并且 与接受标准美国饮食的参与者相比，CRC相关微生物的丰度更高。我们还将 评估功能水平标志物，包括胆汁酸和短链脂肪酸 (SCFA) 的产生和 炎症标志物。如果我们的假设得到支持，我们预计次级胆汁的产生会减少 酸（例如脱氧胆酸）、更多的 SCFA 产生（例如丁酸盐）以及肠道和全身的减少 与标准饮食相比，接受 DASH 饮食的参与者出现炎症（例如钙卫蛋白、IL-6） 美国饮食。我们的研究结果将为 DASH 饮食作为培养能力的方法提供初步证据 不同种族人群的肠道微生物群更健康。这些发现可能会影响临床、转化、 以及改变肠道微生物群的人群水平方法，以降低慢性疾病的风险，例如 CRC。