Determining the structural- and functional-level effects of diet-specific interventions on the gut microbiota of a diverse sample of Southern United States adults
确定特定饮食干预措施对美国南部成年人不同样本肠道微生物群的结构和功能水平影响
基本信息
- 批准号:10361024
- 负责人:
- 金额:$ 38.46万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-06-01 至 2025-05-31
- 项目状态:未结题
- 来源:
- 关键词:AcetatesAddressAdultAffectAmericanAmerican dietBacteroidesBase SequenceBifidobacteriumBile AcidsBiological MarkersBody Weight decreasedButyratesCardiac healthCardiovascular DiseasesCategoriesChronic DiseaseClinicalClostridiumColorectal CancerConsequentialismConsumptionDASH dietDNA DamageDataDeoxycholic AcidDietDiet ModificationDietary FiberDietary InterventionDietary PracticesEnvironmentFatty acid glycerol estersFemaleFiberFusobacteriumGoalsHealthHealth BenefitHealth PromotionHealth StatusIncidenceIndividualInflammationInterleukin-6InterventionKnowledgeLactobacillusLeadLeukocyte L1 Antigen ComplexLinkLiteratureLithocholic AcidMetabolicMetagenomicsMicrobeModificationNot Hispanic or LatinoObesityOutcomeParticipantPathogenicityPhysiciansPhysiologicalPopulationPopulation HeterogeneityPorphyromonasPrevention ResearchPrevention approachPreventiveProductionPropionatesPublic HealthRaceRandomizedRecommendationResearchRibosomal RNARiskRisk FactorsRuminococcusSamplingScientistStructureTaxonomyTestingTherapeuticTimeUnited StatesVolatile Fatty AcidsWorkarmbasebeta diversitybiobehaviorblood pressure reductioncancer riskcolorectal cancer riskcolorectal cancer treatmentdietarydisorder riskdysbiosisefficacy testingexperimental studyfeedingfruits and vegetablesgut bacteriagut microbiotahealth disparityimprovedinflammatory disease of the intestineinflammatory markerinnovationliver inflammationmalemicrobialmicrobiomemortalitynutritionpublic health relevancerRNA Genesracial differenceracial disparityracial diversityrecruitsexsystemic inflammatory response
项目摘要
Racial disparities in colorectal cancer (CRC) incidence persist for blacks and whites. Given the previous
work of our team documenting racial differences in the gut microbiota of blacks and whites and the evidence
supporting the interaction between diet and the gut microbiota as a risk factor for colorectal cancer, the study of
how various diets affect the structure and function of the gut microbiota across racial groups is warranted.
Previous research has shown that the gut microbiota can be rapidly altered by changes in diet. For example,
consumption of an extremely high fiber (>50 grams) diet has produced changes in the gut microbiota that are
believed to reduce cancer risk. The Dietary Approaches to Stop Hypertension (DASH) diet, rich in fruits,
vegetables, whole grains and low-fat dairy, is commonly recommended for heart health and has been shown to
lower blood pressure and produce weight loss. However, to our knowledge, the effect of the DASH diet on the
gut microbiota has not been studied. Because the DASH diet provides substantial fiber, we hypothesize that
consumption of the DASH diet will lead to improvements in the gut microbiota of non-Hispanic black and white
adults. In this proposal, we plan to investigate our hypothesis by recruiting a generally healthy sample of 112
black and white adults from Birmingham, AL to participate in a 28-day randomized, controlled feeding study.
Participants will be randomized to receive either the DASH diet or a standard American diet. All meals will be
provided by the study. Fecal samples will be collected at multiple time points before, during, and after the dietary
intervention and will be analyzed using PCR to amplify the V4 region of the 16S rRNA gene and to sequence
bases using the MiSeq platform. Sequenced data will then be analyzed using QIIME. We hypothesize that
participants receiving the DASH diet will have a greater increase in alpha diversity and greater changes in
abundances of CRC-associated microbes than participants receiving the standard American diet. We will also
evaluate functional-level markers including bile acid and short chain fatty acid (SCFA) production and
inflammatory markers. If our hypothesis is supported, we expect to see reduced production of secondary bile
acids (e.g., deoxycholic acid), greater SCFA production (e.g, butyrate), and reduction in gut and systemic
inflammation (e.g, calprotectin, IL-6) among participants receiving the DASH diet compared to the standard
American diet. Our findings will provide preliminary evidence for the DASH diet as an approach for cultivating
a healthier gut microbiota across racially diverse populations. These findings can impact clinical, translational,
and population-level approaches for modification of the gut microbiota to reduce risk of chronic diseases like
CRC.
黑人和白人结直肠癌 (CRC) 发病率的种族差异仍然存在。鉴于之前的
我们团队的工作记录了黑人和白人肠道微生物群的种族差异以及证据
支持饮食和肠道微生物群之间的相互作用作为结直肠癌的危险因素,该研究
不同的饮食如何影响不同种族群体肠道微生物群的结构和功能是有必要的。
先前的研究表明,肠道微生物群可以通过饮食的变化而迅速改变。例如,
摄入极高纤维(> 50 克)饮食已导致肠道微生物群发生变化,这些变化
据信可以降低癌症风险。预防高血压的饮食方法(DASH)饮食,富含水果,
蔬菜、全谷物和低脂乳制品通常被推荐用于心脏健康,并且已被证明可以
降低血压并减轻体重。然而,据我们所知,DASH 饮食对
尚未研究肠道微生物群。由于 DASH 饮食提供大量纤维,我们假设
食用 DASH 饮食将改善非西班牙裔黑人和白人的肠道微生物群
成年人。在本提案中,我们计划通过招募 112 名总体健康的样本来研究我们的假设。
来自阿拉巴马州伯明翰的黑人和白人成年人参加了一项为期 28 天的随机对照喂养研究。
参与者将被随机分配接受 DASH 饮食或标准美式饮食。所有餐食将
该研究提供。将在饮食之前、期间和之后的多个时间点收集粪便样本
干预,并将使用 PCR 进行分析,以扩增 16S rRNA 基因的 V4 区域并测序
使用 MiSeq 平台的基础。然后使用 QIIME 分析测序数据。我们假设
接受 DASH 饮食的参与者的 α 多样性将有更大的增加,并且
与接受标准美国饮食的参与者相比,CRC相关微生物的丰度更高。我们还将
评估功能水平标志物,包括胆汁酸和短链脂肪酸 (SCFA) 的产生和
炎症标志物。如果我们的假设得到支持,我们预计次级胆汁的产生会减少
酸(例如脱氧胆酸)、更多的 SCFA 产生(例如丁酸盐)以及肠道和全身的减少
与标准饮食相比,接受 DASH 饮食的参与者出现炎症(例如钙卫蛋白、IL-6)
美国饮食。我们的研究结果将为 DASH 饮食作为培养能力的方法提供初步证据
不同种族人群的肠道微生物群更健康。这些发现可能会影响临床、转化、
以及改变肠道微生物群的人群水平方法,以降低慢性疾病的风险,例如
CRC。
项目成果
期刊论文数量(0)
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会议论文数量(0)
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Tiffany LaShaun Carson其他文献
Tiffany LaShaun Carson的其他文献
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{{ truncateString('Tiffany LaShaun Carson', 18)}}的其他基金
Improving weight loss outcomes of black women using a culturally relevant, stress management enhancement behavioral weight loss intervention
使用文化相关的压力管理增强行为减肥干预措施改善黑人女性的减肥结果
- 批准号:
10370478 - 财政年份:2021
- 资助金额:
$ 38.46万 - 项目类别:
Improving weight loss outcomes of black women using a culturally relevant, stress management enhancement behavioral weight loss intervention
使用文化相关的压力管理增强行为减肥干预措施改善黑人女性的减肥结果
- 批准号:
10615192 - 财政年份:2021
- 资助金额:
$ 38.46万 - 项目类别:
Determining the structural- and functional-level effects of diet-specific interventions on the gut microbiota of a diverse sample of Southern United States adults
确定特定饮食干预措施对美国南部成年人不同样本肠道微生物群的结构和功能水平影响
- 批准号:
10225377 - 财政年份:2021
- 资助金额:
$ 38.46万 - 项目类别:
Improving weight loss outcomes of black women using a culturally relevant, stress management enhancement behavioral weight loss intervention
使用文化相关的压力管理增强行为减肥干预措施改善黑人女性的减肥结果
- 批准号:
10457471 - 财政年份:2021
- 资助金额:
$ 38.46万 - 项目类别:
Improving weight loss outcomes of black women using a culturally relevant, stress-management enhanced behavioral weight loss intervention
使用文化相关的压力管理增强行为减肥干预措施改善黑人女性的减肥结果
- 批准号:
10028373 - 财政年份:2020
- 资助金额:
$ 38.46万 - 项目类别:
The Interplay of Behavior, Environment, and Microbiota in Colorectal Cancer Risk
行为、环境和微生物群在结直肠癌风险中的相互作用
- 批准号:
9755367 - 财政年份:2015
- 资助金额:
$ 38.46万 - 项目类别:
The Interplay of Behavior, Environment, and Microbiota in Colorectal Cancer Risk
行为、环境和微生物群在结直肠癌风险中的相互作用
- 批准号:
8967533 - 财政年份:2015
- 资助金额:
$ 38.46万 - 项目类别:
The Interplay of Behavior, Environment, and Microbiota in Colorectal Cancer Risk
行为、环境和微生物群在结直肠癌风险中的相互作用
- 批准号:
9130808 - 财政年份:2015
- 资助金额:
$ 38.46万 - 项目类别:
The impact of weight on quality of life in African American women
体重对非裔美国女性生活质量的影响
- 批准号:
7811432 - 财政年份:2010
- 资助金额:
$ 38.46万 - 项目类别:
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