Immune Dysregulation in Sarcoidosis

结节病的免疫失调

基本信息

  • 批准号:
    10362520
  • 负责人:
  • 金额:
    $ 5.1万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2020
  • 资助国家:
    美国
  • 起止时间:
    2020-04-01 至 2025-03-31
  • 项目状态:
    未结题

项目摘要

Abstract Sarcoidosis is a poorly characterized immune disorder that has often been called the “Great Imitator” due to its similarity to a variety of other diseases and the difficulty of diagnosis. The disease causes aggregates of immune cells called granulomas to form in various tissues, causing organ damage and eventual death in severe cases. Sarcoidosis etiology remains largely unknown, with no clear trigger and symptoms that can disappear on their own or worsen despite treatment. Current research has uncovered associations with some common bacterial species as well as immune dysregulation, especially in the T helper 1, T helper 17, and T regulatory cell populations. However, progress in the field has been hampered in part by the complexity of the disease and limitations in current models. We hypothesize that distinct subpopulations of T cells drive immune dysregulation and progression in sarcoidosis. We further hypothesize that this dysregulation differentiates sarcoidosis from other granulomatous processes and provides a basis for disease specific modeling and drug discovery. Aim 1 will apply recently developed single-cell level sequencing techniques to better elucidate the complex network of gene and cell interactions involved in pathogenesis. Samples of sarcoidosis patient granulomas and peripheral blood mononuclear cells (PBMCs) will be analyzed via single-cell RNA sequencing. Their transcriptomes will be compared to the PBMCs of control subjects as well as control granulomas formed by stimulation of control PBMCs with purified protein derivative. Cells will be identified by their gene expression profiles, then assessed for differences in gene expression and regulation in sarcoidosis compared to controls. We will use this data to develop a transcriptomic atlas of cell types and regulatory networks in sarcoidosis. Aim 2 will use this atlas to evaluate in vitro sarcoidosis models which successfully utilized patient samples to elucidate differences between disease and controls. Granulomas created using each model will be analyzed via single-cell RNA-seq and compared to our sarcoidosis transcriptomic atlas. This approach fosters detailed evaluation of the sarcoidosis immune environment and the evaluation of the capability of existing sarcoidosis models to mimic sarcoidosis. This project integrates recent advances in sequencing and bioinformatics to uncover mechanisms involved in sarcoidosis. The single-cell transcriptomic atlas of sarcoidosis granulomas and PBMCs will interrogate cellular interactions that regulate immune dysfunction in sarcoidosis, providing new points of focus for further mechanistic research. Our analysis of sarcoidosis models will guide further model design, and design of high throughput biomarker and drug screens for sarcoidosis. This project has the potential to improve the specificity and efficacy of treatment, as well as the ease of diagnosis. The multidisciplinary research also lays the groundwork for my training as a physician scientist while exploring a key clinical question.
摘要

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Kai Huang其他文献

Kai Huang的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Kai Huang', 18)}}的其他基金

Immune Dysregulation in Sarcoidosis
结节病的免疫失调
  • 批准号:
    9910664
  • 财政年份:
    2020
  • 资助金额:
    $ 5.1万
  • 项目类别:
Immune Dysregulation in Sarcoidosis
结节病的免疫失调
  • 批准号:
    10590750
  • 财政年份:
    2020
  • 资助金额:
    $ 5.1万
  • 项目类别:
Immune Dysregulation in Sarcoidosis
结节病的免疫失调
  • 批准号:
    10378694
  • 财政年份:
    2020
  • 资助金额:
    $ 5.1万
  • 项目类别:

相似国自然基金

层出镰刀菌氮代谢调控因子AreA 介导伏马菌素 FB1 生物合成的作用机理
  • 批准号:
    2021JJ40433
  • 批准年份:
    2021
  • 资助金额:
    0.0 万元
  • 项目类别:
    省市级项目
寄主诱导梢腐病菌AreA和CYP51基因沉默增强甘蔗抗病性机制解析
  • 批准号:
    32001603
  • 批准年份:
    2020
  • 资助金额:
    24.0 万元
  • 项目类别:
    青年科学基金项目
AREA国际经济模型的移植.改进和应用
  • 批准号:
    18870435
  • 批准年份:
    1988
  • 资助金额:
    2.0 万元
  • 项目类别:
    面上项目

相似海外基金

Onboarding Rural Area Mathematics and Physical Science Scholars
农村地区数学和物理科学学者的入职
  • 批准号:
    2322614
  • 财政年份:
    2024
  • 资助金额:
    $ 5.1万
  • 项目类别:
    Standard Grant
Point-scanning confocal with area detector
点扫描共焦与区域检测器
  • 批准号:
    534092360
  • 财政年份:
    2024
  • 资助金额:
    $ 5.1万
  • 项目类别:
    Major Research Instrumentation
TRACK-UK: Synthesized Census and Small Area Statistics for Transport and Energy
TRACK-UK:交通和能源综合人口普查和小区域统计
  • 批准号:
    ES/Z50290X/1
  • 财政年份:
    2024
  • 资助金额:
    $ 5.1万
  • 项目类别:
    Research Grant
Wide-area low-cost sustainable ocean temperature and velocity structure extraction using distributed fibre optic sensing within legacy seafloor cables
使用传统海底电缆中的分布式光纤传感进行广域低成本可持续海洋温度和速度结构提取
  • 批准号:
    NE/Y003365/1
  • 财政年份:
    2024
  • 资助金额:
    $ 5.1万
  • 项目类别:
    Research Grant
Collaborative Research: Scalable Manufacturing of Large-Area Thin Films of Metal-Organic Frameworks for Separations Applications
合作研究:用于分离应用的大面积金属有机框架薄膜的可扩展制造
  • 批准号:
    2326714
  • 财政年份:
    2024
  • 资助金额:
    $ 5.1万
  • 项目类别:
    Standard Grant
Collaborative Research: Scalable Manufacturing of Large-Area Thin Films of Metal-Organic Frameworks for Separations Applications
合作研究:用于分离应用的大面积金属有机框架薄膜的可扩展制造
  • 批准号:
    2326713
  • 财政年份:
    2024
  • 资助金额:
    $ 5.1万
  • 项目类别:
    Standard Grant
Unlicensed Low-Power Wide Area Networks for Location-based Services
用于基于位置的服务的免许可低功耗广域网
  • 批准号:
    24K20765
  • 财政年份:
    2024
  • 资助金额:
    $ 5.1万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
RAPID: Collaborative Research: Multifaceted Data Collection on the Aftermath of the March 26, 2024 Francis Scott Key Bridge Collapse in the DC-Maryland-Virginia Area
RAPID:协作研究:2024 年 3 月 26 日 DC-马里兰-弗吉尼亚地区 Francis Scott Key 大桥倒塌事故后果的多方面数据收集
  • 批准号:
    2427233
  • 财政年份:
    2024
  • 资助金额:
    $ 5.1万
  • 项目类别:
    Standard Grant
Postdoctoral Fellowship: OPP-PRF: Tracking Long-Term Changes in Lake Area across the Arctic
博士后奖学金:OPP-PRF:追踪北极地区湖泊面积的长期变化
  • 批准号:
    2317873
  • 财政年份:
    2024
  • 资助金额:
    $ 5.1万
  • 项目类别:
    Standard Grant
RAPID: Collaborative Research: Multifaceted Data Collection on the Aftermath of the March 26, 2024 Francis Scott Key Bridge Collapse in the DC-Maryland-Virginia Area
RAPID:协作研究:2024 年 3 月 26 日 DC-马里兰-弗吉尼亚地区 Francis Scott Key 大桥倒塌事故后果的多方面数据收集
  • 批准号:
    2427232
  • 财政年份:
    2024
  • 资助金额:
    $ 5.1万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了