Persistence of SARS-CoV-2 in Wastewater

严重急性呼吸综合征冠状病毒-2(SARS-CoV-2)是冠状病毒家族的成员， 最近从武汉脱颖而出的中国。世界卫生组织(WHO)宣布了 [冠状病毒病2019年(新冠肺炎)]，并将其列为全球大流行。尽管病毒是 主要通过人与人之间的接触传播，并导致呼吸道疾病；最近的一些研究 提出了粪便经口腔传播的可能性，并在感染者的粪便中检测到RNA病毒 个人，即使在呼吸道症状消退后也是如此。最近的一项研究证实，这种病毒也是 能够感染人类肠道肠道细胞(Lamers等人，2020年)。然而，SARS的潜在传播- CoV-2通过废水传播目前尚不清楚。因此，建议在这里进行的研究将有助于更好地 污水中SARS-CoV-2的持久性和消毒的认识。首先，我们将使用实验室- 检查废水中病毒的生存能力的对照研究。我们将把SARS-CoV-2病毒 随着时间的推移，监测废水的传染性。第二，我们将使用含氯消毒剂来确定 废水中SARS-CoV-2的灭活。最后，我们将进行实地调查，调查病毒的命运 来自不同地区的废水处理系统。拟议的工作将向各州和 可用于制定新冠肺炎预防指南的联邦监管机构。该项目将 还帮助评估和开发最佳消毒方法和量化微生物风险 评估(QMRA)。这些发现将对水资源回收设施进行更好的管理具有不可估量的价值 防止潜在的接触风险，确保工人的健康和安全。

Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), member of the Coronavirus family, has recently emerged from Wuhan, China. The World Health Organization (WHO) announced an official name of the disease [coronavirus disease 2019 (COVID-19)] and classified it as a global pandemic. Though the virus is primarily spread through person-to-person contact and causes respiratory tract illnesses; some recent studies have suggested the possibility for fecal-oral transmission and detected the RNA virus in the feces of infected individuals, even after respiratory symptoms have subsided. A recent study confirmed that the virus is also capable of infecting human gut enterocytes (Lamers et al., 2020). However, the potential transmission of SARS- CoV-2 via wastewater is currently unknown. Therefore, the proposed study here will facilitate a better understanding of persistence and disinfection of SARS-CoV-2 in wastewater. First, we will use laboratory- controlled studies examining the survivability of the virus in wastewater. We will spike the SARS-CoV-2 in wastewater and monitor infectivity over time. Second, we will use chlorine disinfectants to determine the inactivation of SARS-CoV-2 in wastewater. Last, we will conduct a field survey to investigate the fate of the virus in wastewater treatment systems from different locales. The proposed work will provide information to state and federal regulatory agencies that can be used to develop guidance for prevention of COVID-19. The project will also aid in evaluation and development of best disinfection approaches and quantitative microbial risk assessment (QMRA). The findings will be invaluable to water resource recovery facilities for better management against potential exposure risk and ensuring worker health and safety.