Persistence of SARS-CoV-2 in Wastewater

严重急性呼吸道综合征冠状病毒2型（SARS-CoV-2）是冠状病毒家族的成员， 最近出现在中国武汉。世界卫生组织（WHO）宣布了一个正式名称， 疾病[2019冠状病毒病（COVID-19）]，并将其归类为全球大流行病。虽然病毒是 主要通过人与人之间的接触传播，并导致呼吸道疾病;最近的一些研究 提出粪-口传播的可能性，并在感染者的粪便中检测到RNA病毒 即使在呼吸道症状消退后。最近的一项研究证实，该病毒也是 能够感染人肠上皮细胞（Lamers等，2020年）。然而，SARS的潜在传播- CoV-2通过废水是目前未知的。因此，建议的研究将有助于更好地 了解废水中SARS-CoV-2的持久性和消毒。首先，我们将使用实验室- 对照研究检测病毒在废水中的存活能力。我们会把SARS病毒注入 废水和监测传染性随着时间的推移。其次，我们将使用氯消毒剂来确定 废水中SARS-CoV-2灭活。最后，我们将进行实地调查，调查病毒的命运 在不同地区的废水处理系统中。拟议的工作将向各国提供信息， 可用于制定COVID-19预防指南的联邦监管机构。该项目将 还有助于评估和开发最佳消毒方法和定量微生物风险 评估（QMRA）。研究结果对水资源回收设施进行更好的管理将是非常宝贵的 防止潜在的接触风险，确保工人的健康和安全。

Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), member of the Coronavirus family, has recently emerged from Wuhan, China. The World Health Organization (WHO) announced an official name of the disease [coronavirus disease 2019 (COVID-19)] and classified it as a global pandemic. Though the virus is primarily spread through person-to-person contact and causes respiratory tract illnesses; some recent studies have suggested the possibility for fecal-oral transmission and detected the RNA virus in the feces of infected individuals, even after respiratory symptoms have subsided. A recent study confirmed that the virus is also capable of infecting human gut enterocytes (Lamers et al., 2020). However, the potential transmission of SARS- CoV-2 via wastewater is currently unknown. Therefore, the proposed study here will facilitate a better understanding of persistence and disinfection of SARS-CoV-2 in wastewater. First, we will use laboratory- controlled studies examining the survivability of the virus in wastewater. We will spike the SARS-CoV-2 in wastewater and monitor infectivity over time. Second, we will use chlorine disinfectants to determine the inactivation of SARS-CoV-2 in wastewater. Last, we will conduct a field survey to investigate the fate of the virus in wastewater treatment systems from different locales. The proposed work will provide information to state and federal regulatory agencies that can be used to develop guidance for prevention of COVID-19. The project will also aid in evaluation and development of best disinfection approaches and quantitative microbial risk assessment (QMRA). The findings will be invaluable to water resource recovery facilities for better management against potential exposure risk and ensuring worker health and safety.