MHC Genetic Typing Core

MHC 基因分型核心

• 批准号: 10373935
• 负责人: Amanda Vinson
• 金额: $ 17.32万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-01-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10373935
• 关键词: Acquired Immunodeficiency Syndrome; Algorithms; Alleles; Animal Husbandry; Animal Model; Animals; Biological Assay; Biomedical Research; Breeding; Caring; Data; Development; Disease; Disease Progression; Ensure; Gene Frequency; Genetic; Genetic Variation; Genomics; Genotype; Goals; HIV; Health; Individual; Knowledge; Lead; MHC Class I Genes; Macaca; Macaca mulatta; Morbidity - disease rate; Periodicity; Phenotype; Population; Practice Management; Primates; Protocols documentation; Recording of previous events; Reporting; Research; SIV; Silver; Technology; Time; United States; Work; animal colony; base; cost; cost effective; demographics; experience; genetic pedigree; genetic testing; genome-wide; human disease; improved; innovation; interest; member; mortality; nonhuman primate; novel; offspring; preservation; recruit; success; trait; working group

PROJECT SUMMARY, Core C: MHC Genetic Typing Core Genetically well-characterized animal models are critical for the success of S/HIV research, including characterization for MHC genotype, known ancestry, and pedigree relationships. In particular, informative and accurate MHC genotyping is essential, due to the complexity of the MHC class I loci in macaques, and the influence of allele type on disease progression. The need for genetically well-characterized animal models for research must also be balanced against the need to preserve genetic diversity in animal colonies dedicated to supporting biomedical research. This is a requirement not only to ensure long-term colony health, but to ensure the relevance of experimental findings to human disease. The ONPRC is a leader in the development of many of the current recommended approaches and technologies used in genetic typing and management of NHP colonies. Thus, the aims of the Genetics Core for this project are to, 1) use state-of-the-art genetic testing to characterize all ONPRC Indian-origin rhesus macaques for MHC genotype, and 2) to maximize the genetic diversity of the ONPRC breeding colony while producing sufficient numbers of MHC-genotyped Indian-origin rhesus macaques to meet research needs. To achieve these aims, we will conduct MHC Class I genotyping of an additional 1,760 SPF4 Indian-origin rhesus macaques to support continued research, and will additionally develop new or improved approaches to the genetic management of these macaques. These innovations include the use of cost-effective genome-wide sparse sequencing to assess pedigree relationships, genetic diversity, and ancestry in a single, combined assay; the implementation of automated, periodic reporting aimed at summarizing genetic diversity for every breeding group in the colony; and improved algorithms for estimating the genetic value of individual animals by adding information on demographics, genotypes or phenotypes of interest, and animal assignment history.

项目总结，核心C：MHC基因分型核心 基因特征良好的动物模型对于S/HIV研究的成功至关重要，包括 MHC基因型、已知祖先和系谱关系的表征。特别是， 准确的MHC基因分型是必不可少的，因为猕猴中MHC I类基因座的复杂性， 等位基因类型对疾病进展影响。需要遗传上良好表征的动物模型， 研究还必须与保护动物群体遗传多样性的需要相平衡， 支持生物医学研究。这不仅是为了确保长期的菌落健康， 实验结果与人类疾病的相关性。ONPRC是许多领域开发的领导者 目前推荐的用于NHP基因分型和管理的方法和技术 殖民地因此，该项目的遗传学核心的目标是：1）使用最先进的基因检测， 表征所有ONPRC印度起源恒河猴的MHC基因型，和2）最大化遗传 ONPRC繁殖群体的多样性，同时产生足够数量的MHC基因分型的印度起源 恒河猴，以满足研究需要。为了实现这些目标，我们将进行MHC I类基因分型， 增加1，760只SPF 4印度恒河猴，以支持继续研究，并将额外 开发新的或改进的方法来对这些猕猴进行遗传管理。这些创新包括 使用具有成本效益的全基因组稀疏测序来评估谱系关系，遗传多样性， 在一个单一的，组合的分析祖先;实施自动化，定期报告，旨在总结 殖民地中每个育种群体的遗传多样性，以及估计遗传值的改进算法 通过添加关于感兴趣的人口统计学、基因型或表型的信息， 任务历史