Component: MHC Genetic Core

成分：MHC 遗传核心

基本信息

批准号：
10666558
负责人：
Amanda Vinson
金额：
$ 7.45万
依托单位：
OREGON HEALTH & SCIENCE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-08-01 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10666558
关键词：
Acquired Immunodeficiency Syndrome Address Adult Age Algorithms Alleles Animal Husbandry Animal Model Animals Award Base Sequence Biological Assay Biomedical Research Breeding Caring Dedications Development Disease Disease Progression Ensure Female Gene Frequency Genetic Genetic Variation Genome Genomics Genotype Goals HIV Health Immigrant Individual Infant Informatics Knowledge Lead MHC Class I Genes Macaca Macaca mulatta Maintenance Morbidity - disease rate Practice Management Primates Protocols documentation Recommendation Reporting Research Research Support SNP genotyping Technology Time Uncertainty Work animal breeding animal colony cost experience feasibility testing genetic pedigree genetic testing genome-wide human disease improved male member mortality novel offspring preservation recruit simian human immunodeficiency virus success trait working group

项目摘要

PROJECT SUMMARY, Core C: MHC Genetic Typing Core Genetically well-characterized animal models are critical for the success of HIV/SHIV research, including characterization for MHC genotype, known ancestry, and pedigree relationships. In particular, informative and accurate MHC genotyping is essential, due to the complexity of the MHC class I loci in macaques, and the influence of allele type on disease progression. The need for genetically well-characterized animal models for research must also be balanced against the need to preserve genetic diversity in animal colonies dedicated to supporting biomedical research. This is a requirement not only to ensure long-term colony health, but to ensure the relevance of experimental findings to human disease. The ONPRC is a leader in the development in many of the current recommended approaches and technologies used in genetic typing and management of NHP colonies. The aims of the Genetics Core for this project are to 1) use state-of-the-art genetic testing to produce colony offspring of known parentage, ancestry, and MHC genotype and 2) to maintain the genetic diversity of this extended SPF colony while producing sufficient numbers of offspring to meet research needs. We will continue to use the Fluidigm 96-SNP parentage array to assign parentage. All new colony offspring will be typed for MHC Class I alleles using a sequence-based expressed allele assay. We will also conduct genetic management using new protocols developed to assign genetic value for each animal, and to propose breeding groups that will minimize the loss of genetic diversity in the colony. Lastly, we will develop new informatics protocols to support these efforts, including the consideration of MHC type in the maintenance of genetic diversity.

项目摘要，核心C：MHC遗传打字核心遗传性良好的动物模型对于艾滋病毒/湿婆研究的成功至关重要，包括 MHC基因型，已知血统和谱系关系的表征。特别是信息丰富的准确的MHC基因分型至关重要，这是由于MHC I类位点在猕猴中的复杂性，并且是必不可少的。等位基因类型对疾病进展的影响。需要遗传良好的动物模型研究还必须与维护专门用于的动物菌落中的遗传多样性的需求保持平衡。支持生物医学研究。这不仅是确保长期殖民地健康的要求，而且要确保实验发现与人类疾病的相关性。 ONPRC是许多人发展的领导者在NHP的遗传分键和管理中使用的当前推荐方法和技术殖民地。该项目的遗传学核心的目的是1）使用最先进的基因检测来生产已知亲属，祖先和MHC基因型的殖民地后代以及2）维持的遗传多样性这种扩展的SPF菌落在产生足够数量的后代以满足研究需求的同时。我们将继续使用fluidigm 96-SNP parentage数组来分配parthage。所有新的殖民地后代将是使用基于序列的表达等位基因测定法为MHC I类等位基因键入。我们还将进行遗传管理使用新方案为每只动物分配遗传价值的管理，并提出育种将最小化菌落中遗传多样性丧失的群体。最后，我们将开发新的信息学支持这些努力的协议，包括在维持遗传方面考虑MHC类型多样性。