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Component: MHC Genetic Core

成分：MHC 遗传核心

基本信息

批准号：
10338460
负责人：
Amanda Vinson
金额：
$ 7.45万
依托单位：
OREGON HEALTH & SCIENCE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-08-01 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10338460
关键词：
Acquired Immunodeficiency Syndrome Address Adult Age Algorithms Alleles Animal Husbandry Animal Model Animals Award Base Sequence Biological Assay Biomedical Research Breeding Caring Development Disease Disease Progression Ensure Female Gene Frequency Genetic Genetic Variation Genome Genomics Genotype Goals HIV Health Immigrant Individual Infant Informatics Knowledge Lead MHC Class I Genes Macaca Macaca mulatta Maintenance Morbidity - disease rate Practice Management Primates Protocols documentation Reporting Research Research Support SNP genotyping Technology Time Uncertainty Work animal breeding animal colony cost experience feasibility testing genetic pedigree genetic testing genome-wide human disease improved male member mortality novel offspring preservation recruit simian human immunodeficiency virus success trait working group

项目摘要

PROJECT SUMMARY, Core C: MHC Genetic Typing Core Genetically well-characterized animal models are critical for the success of HIV/SHIV research, including characterization for MHC genotype, known ancestry, and pedigree relationships. In particular, informative and accurate MHC genotyping is essential, due to the complexity of the MHC class I loci in macaques, and the influence of allele type on disease progression. The need for genetically well-characterized animal models for research must also be balanced against the need to preserve genetic diversity in animal colonies dedicated to supporting biomedical research. This is a requirement not only to ensure long-term colony health, but to ensure the relevance of experimental findings to human disease. The ONPRC is a leader in the development in many of the current recommended approaches and technologies used in genetic typing and management of NHP colonies. The aims of the Genetics Core for this project are to 1) use state-of-the-art genetic testing to produce colony offspring of known parentage, ancestry, and MHC genotype and 2) to maintain the genetic diversity of this extended SPF colony while producing sufficient numbers of offspring to meet research needs. We will continue to use the Fluidigm 96-SNP parentage array to assign parentage. All new colony offspring will be typed for MHC Class I alleles using a sequence-based expressed allele assay. We will also conduct genetic management using new protocols developed to assign genetic value for each animal, and to propose breeding groups that will minimize the loss of genetic diversity in the colony. Lastly, we will develop new informatics protocols to support these efforts, including the consideration of MHC type in the maintenance of genetic diversity.

项目总结，核心C: MHC基因分型核心