Precision Nutrition Core

项目摘要-Precision Nutrition Core Precision Nutrition Core成立于2016年，作为试点核心，仅提供营养基因组学服务。 虽然遗传学在精确营养中的作用已经被广泛接受，但该领域才刚刚开始达到它的水平。 潜在的，相对较少的营养-基因相互作用的例子已经达成共识。此外， 尚未完成这些调查结果的翻译，并继续进行强有力的调查，由 需要具备必要专业知识的研究人员。核心设计的一个核心原则是我们可以最好地 满足UNC NORC成员的需求，与实质性专家密切合作，扩大他们的 研究营养反应中的变异性。自成立以来，核心的用户基础已经 持续增加，从2016年的18个用户增加到2019年的42个用户，用户关于服务的查询也在不断增加 与精确营养的其他方面--特别是肠道微生物群--有关。因此，核心部门招聘了 Carroll博士将实施和监督以营养和肥胖为重点的微生物组服务，这是以前从未满足过的 北卡罗来纳大学需要。肠道微生物群通过其对代谢异质性的影响与代谢异质性密切相关。 能量获取、代谢信号和饮食成分代谢物的产生。这个核心是一个 在整个国家NORC系统中只有两个核心提供营养基因组学服务。的目标是 精准营养的核心是提供一套精准的营养服务，使研究人员能够将 营养基因组学和微生物组分析在营养与肥胖研究中的应用 个体遗传学和肠道微生物群落如何影响与营养相关的个体变异性 暴露和肥胖。该中心在两个领域提供专业知识和服务：1)营养基因组学和2) 微生物组分析。核心提供实验室服务以评估个体变异--包括DNA 从血液、唾液和粪便样本中提取RNA，并测定粪便样本的热量含量 和粪便代谢物评估-以及定制的尖端研究工具-包括 基因组、外显子组和靶向基因测序；RNA测序；微生物区系特征，包括16S RRNA基因测序和鸟枪式元基因组学；基于聚合酶链式反应的靶向基因分型；阵列- 基于基因表达谱和二硫键测序的人类和小鼠甲基化模式 模特们。利用UNC NORC的可用资源，核心填补了营养和营养方面的严重缺口 通过为研究人员提供专业知识和资源来研究肥胖在北卡罗来纳大学的作用 宿主遗传和肠道微生物组因素在营养暴露的生物学反应中的作用。此核心位于 教堂山和北卡罗来纳大学营养研究所都在校园内为研究人员提供服务 地点。

Project Summary – Precision Nutrition Core The Precision Nutrition Core was established in 2016 as a pilot core, offering nutrigenomics services only. While the role of genetics in precision nutrition has been widely accepted, the field has only begun to reach its potential, and relatively few examples of nutrient-gene interactions have reached consensus. Furthermore, the translation of these findings has not been fulfilled, and continued robust investigations, conducted by researchers with necessary expertise, are needed. A central tenet in the design of the Core is that we can best meet the demands of UNC NORC members by working closely with substantive experts to expand their research into studies of variability in nutritional response. Since its inception, the Core’s user base has increased consistently, from 18 users in 2016 to 42 users in 2019, as have user inquiries regarding services related to other aspects of precision nutrition—in particular, the gut microbiome. As such, the Core recruited Dr. Carroll to implement and oversee nutrition- and obesity-focused microbiome services, a previously unmet need at UNC. The gut microbiome is intimately connected to metabolic heterogeneity through its effects on energy harvest, metabolic signaling, and the production of metabolites of dietary components. This Core is one of only two cores across the national NORC system to provide nutrigenomics services. The goal of the Precision Nutrition Core is to provide a suite of precision nutrition services that enable investigators to integrate nutrigenomics and microbiome analysis into nutrition and obesity research to advance our understanding of how individual genetics and gut microbial communities influence individual variability related to nutritional exposures and adiposity. The Core provides expertise and services in two areas: 1) Nutrigenomics and 2) Microbiome Analysis. The Core offers laboratory services for assessing individual variation – including DNA and RNA extraction from blood, saliva, and fecal samples and caloric content determination of fecal samples and fecal metabolite assessment – as well as customized, cutting-edge research tools – including whole genome, exome, and targeted gene sequencing; RNA-sequencing; microbiota characterization, including 16S rRNA gene sequencing and shotgun metagenomics; targeted genotyping using PCR-based assays; array- based gene expression profiling; and bisulfide sequencing for methylation patterns in humans and mouse models. Leveraging the resources available at the UNC NORC, the Core fills a critical gap in nutrition and obesity research at UNC by providing investigators with the expertise and resources to investigate the role of host genetic and gut microbiome factors in biological responses to nutritional exposure. This Core is located at both the Chapel Hill and the UNC Nutrition Research Institute campuses to serve investigators at both locations.