Precision Nutrition Core

项目概要-精准营养核心 Precision Nutrition Core成立于2016年，作为试点核心，仅提供营养基因组学服务。 虽然遗传学在精确营养中的作用已被广泛接受，但该领域才刚刚开始达到其 潜在的，和相对较少的例子营养基因相互作用已达成共识。而且 这些调查结果的翻译尚未完成，并继续进行强有力的调查， 需要具备必要专业知识的研究人员。核心设计的一个核心原则是， 通过与实质性专家密切合作， 研究营养反应的变异性。自成立以来，Core的用户群 从2016年的18个用户持续增加到2019年的42个用户，用户对服务的查询也是如此 与精确营养的其他方面有关，特别是肠道微生物组。因此，核心招募了 博士卡罗尔实施和监督营养和肥胖为重点的微生物组服务，以前没有满足 我需要你。肠道微生物组通过其对代谢异质性的影响与代谢异质性密切相关。 能量收获、代谢信号和饮食组分的代谢物的产生。这个核心是一个 在全国NORC系统中，只有两个核心提供营养基因组学服务。的目标 精准营养核心是提供一套精准营养服务，使调查人员能够整合 营养基因组学和微生物组学分析纳入营养和肥胖研究， 个体遗传学和肠道微生物群落如何影响与营养相关的个体差异 暴露和肥胖。核心提供两个领域的专业知识和服务：1）营养基因组学和2） 微生物组分析。核心提供评估个体差异的实验室服务-包括DNA 从血液、唾液和粪便样品中提取RNA，并测定粪便样品的卡路里含量 和粪便代谢物评估-以及定制的，尖端的研究工具-包括整个 基因组、外显子组和靶向基因测序; RNA测序;微生物群表征，包括16 S rRNA基因测序和鸟枪宏基因组学;使用基于PCR的测定进行靶向基因分型;阵列- 基于基因表达谱分析;以及人类和小鼠甲基化模式的二硫键测序 模型核心小组利用北部非洲地区和自然资源中心的现有资源，填补了营养方面的一个关键缺口， 通过为研究人员提供专业知识和资源， 宿主遗传和肠道微生物组因素对营养暴露的生物反应。该核心位于 在查佩尔山和营养研究所的校园， 地点

Project Summary – Precision Nutrition Core The Precision Nutrition Core was established in 2016 as a pilot core, offering nutrigenomics services only. While the role of genetics in precision nutrition has been widely accepted, the field has only begun to reach its potential, and relatively few examples of nutrient-gene interactions have reached consensus. Furthermore, the translation of these findings has not been fulfilled, and continued robust investigations, conducted by researchers with necessary expertise, are needed. A central tenet in the design of the Core is that we can best meet the demands of UNC NORC members by working closely with substantive experts to expand their research into studies of variability in nutritional response. Since its inception, the Core’s user base has increased consistently, from 18 users in 2016 to 42 users in 2019, as have user inquiries regarding services related to other aspects of precision nutrition—in particular, the gut microbiome. As such, the Core recruited Dr. Carroll to implement and oversee nutrition- and obesity-focused microbiome services, a previously unmet need at UNC. The gut microbiome is intimately connected to metabolic heterogeneity through its effects on energy harvest, metabolic signaling, and the production of metabolites of dietary components. This Core is one of only two cores across the national NORC system to provide nutrigenomics services. The goal of the Precision Nutrition Core is to provide a suite of precision nutrition services that enable investigators to integrate nutrigenomics and microbiome analysis into nutrition and obesity research to advance our understanding of how individual genetics and gut microbial communities influence individual variability related to nutritional exposures and adiposity. The Core provides expertise and services in two areas: 1) Nutrigenomics and 2) Microbiome Analysis. The Core offers laboratory services for assessing individual variation – including DNA and RNA extraction from blood, saliva, and fecal samples and caloric content determination of fecal samples and fecal metabolite assessment – as well as customized, cutting-edge research tools – including whole genome, exome, and targeted gene sequencing; RNA-sequencing; microbiota characterization, including 16S rRNA gene sequencing and shotgun metagenomics; targeted genotyping using PCR-based assays; array- based gene expression profiling; and bisulfide sequencing for methylation patterns in humans and mouse models. Leveraging the resources available at the UNC NORC, the Core fills a critical gap in nutrition and obesity research at UNC by providing investigators with the expertise and resources to investigate the role of host genetic and gut microbiome factors in biological responses to nutritional exposure. This Core is located at both the Chapel Hill and the UNC Nutrition Research Institute campuses to serve investigators at both locations.