HIGH-FRUCTOSE MEAL AND GENE EXPRESSION OF APPETITE-RELATED PEPTIDES IN BABOONS

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 该项目的目的是评估高果糖与高葡萄糖挑战对狒狒食欲相关激素基因表达（血浆、脑脊液和mRNA）的影响。最近肥胖症的增加似乎阻碍了高果糖食品消费的增加，特别是高果糖玉米糖浆。果糖通过其对食欲的影响与体重增加有关。果糖会提高血糖，但不会像葡萄糖那样引起饱腹感。与葡萄糖不同，摄入果糖不会导致瘦素和胰岛素水平（增加饱腹感的激素）增加。同样，它也不会像葡萄糖一样抑制生长激素释放肽（一种增加食物摄入量的激素）。这可能会导致卡路里和糖的过度消耗，从而导致体重增加。对啮齿动物和恒河猴的研究发现，果糖摄入量的增加与腹部肥胖的增加有关。 使用狒狒作为我们的研究模型，我们将扩大高果糖饮食的影响的调查，包括其在基因表达水平上的影响。 将使用9只狒狒（3只喂食高果糖餐，3只喂食高葡萄糖餐，3只对照），年龄和大小匹配，无胃肠道病变、炎症或糖尿病史。将评价食欲相关激素的基因表达（蛋白质和mRNA）。本研究将确定狒狒在良好控制条件下长期高果糖与高葡萄糖消耗的代谢效应。从本研究中获得的初步数据将用于建立狒狒模型，以研究果糖与葡萄糖消耗对食欲调节相关神经内分泌因子的差异影响，作为目前正在开发的更大R 01应用的一部分。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The aim of this project is to evaluate the effects of a high-fructose vs. a high-glucose challenge on gene expression (plasma, cerebrospinal fluid and mRNA) of appetite-related hormones in baboons. The recent increase in obesity seems to have paralleled the increase in consumption of foods high in fructose, particularly high-fructose corn syrup. Fructose is associated with body weight gain through its effects on appetite. Fructose raises the blood sugar but does not cause a feeling of fullness in a way that glucose does. Unlike glucose, ingestion of fructose does not cause an increase in leptin and insulin levels (hormones that increase satiety). Similarly it does not suppress ghrelin (a hormone which increases food intake) in the same proportion as glucose. This can cause over consumption of calories and sugar and hence lead to weight gain. Studies in rodents and rhesus macaques have found increased consumption of fructose to be associated with an increase in abdominal adiposity. Using the baboon as our model for study we will expand the investigation of the effects of a high-fructose diet to include its effect at the level of gene expression. Nine baboons (3 fed a high fructose meal, 3 fed a high glucose meal, and 3 control), matched for age and size with no history of gastrointestinal pathologies, inflammation, or diabetes will be utilized. Gene expression (protein and mRNA) of appetite-related hormones will be evaluated. This study will determine the metabolic effects of chronic high-fructose vs. high glucose consumption under well-controlled conditions in the baboon. The preliminary data obtained from this study will be used to establish the baboon as the model for investigating the differential effects of fructose vs. glucose consumption on neuroendocrine factors involved in appetite regulation as part of a larger R01 application now in development.