CRR LIGAND ASSAY AND ANALYSIS CORE

CRR 配体测定和分析核心

• 批准号: 10378077
• 负责人: Christopher Rolland McCartney
• 金额: $ 41.84万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-13 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10378077
• 关键词: Award; Biological Assay; Clinical; Consultations; Core Facility; Data; Data Analyses; Ensure; Fertility; Individual; Infertility; Ligands; Location; Measurement; Measures; Methodology; Methods; National Institute of Child Health and Human Development; Nature; Performance; Polycystic Ovary Syndrome; Publications; Reproducibility; Research; Research Personnel; Services; Standardization; Testing; Training; Visually Impaired Persons; cost effective; data reduction; instrumentation; programs; quality assurance; reproductive; reproductive hormone

The accurate and reliable measurement of reproductive hormones and related analytes are essential for successful research throughout the Fertility and Infertility Branch (FIB) of the NICHD and beyond. The Ligand Assay and Analysis Core of the Clinical and Basic Studies in Polycystic Ovary Syndrome group has been providing this service for over two decades. This open-access core is critical for our and other NCTRI centers, as well as an array of individual and program-type awards made through FIB, broader NICHD and other approved users. The Core dedicates considerable effort to characterizing each assay and to continually reviewing important parameters for each test to maintain rigor, reproducibility and optimal assay performance. The Core has continually evolved, both implementing well-characterized, state-of-the-art assay methods and developing new in-house methodologies to meet the needs of investigators. The Core provides a centralized location with a highly-trained technical staff and the appropriate instrumentation to maintain exceptional standardized methods. This reduces unnecessary and expensive redundancy of effort by other investigators and provides the further advantage that individuals blinded as to the experimental details perform the analysis. The Core's emphasis on maintaining appropriate quality assurance measures ensures analyte data avoids assay reliability pitfalls that may compromise validity and the scientific interpretation of the findings. In addition to providing high-quality, cost-effective assay and analysis services, and developing new methods, the core provides assistance and consultation on assay methodology, data reduction/analysis and data interpretation to investigators that access our Ligand Core Facility. The utility and essential nature of this core to reproductive research is demonstrated by its contribution to 119 primary publications and four reviews since 2014.

准确可靠地测量生殖激素和相关分析物对于 在NICHD的生育和不孕分支（FIB）及其他领域的成功研究。配体 多囊卵巢综合征组的临床和基础研究的测定和分析核心 提供这项服务超过二十年。这个开放获取的核心对我们和其他NCTRI中心至关重要， 以及通过FIB、更广泛的NICHD和其他机构获得的一系列个人和项目类型的奖项。 批准的用户。核心投入了相当大的努力来表征每个测定，并不断 审查每个测试的重要参数，以保持严格性、重现性和最佳的分析性能。 Core不断发展，既实施了表征良好的、最先进的分析方法， 开发新的内部方法，以满足调查人员的需要。核心提供了一个集中的 拥有训练有素的技术人员和适当的仪器设备， 标准化方法。这减少了其他调查人员不必要和昂贵的重复工作 并且提供了另一个优点，即对实验细节不知情的个体进行分析。 核心强调保持适当的质量保证措施，确保分析物数据避免 检测可靠性陷阱，可能会损害有效性和科学解释的结果。此外 提供高质量，具有成本效益的测定和分析服务，并开发新的方法，核心 提供分析方法、数据简化/分析和数据解释方面的协助和咨询， 进入我们配体核心设施的调查员这一核心对生殖的效用和本质 自2014年以来，其对119份主要出版物和4份评论的贡献证明了研究。