Bioanalytical Core

生物分析核心

• 批准号: 10377894
• 负责人: SHIRISH S BARVE
• 金额: $ 35.6万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-10 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10377894
• 关键词: Area; Attention; Award; Biochemical; Biological; Biological Assay; Cells; Centers of Research Excellence; Clinical Research; Communities; Computer software; Continuing Education; Core Facility; Core Grant; Data Analyses; Development; Drug Interactions; Ensure; Environment; Equipment; Evaluation; Extramural Activities; Faculty; Fee-for-Service Plans; Felis catus; Fostering; Funding; Future; Genus Hippocampus; Goals; Grant; HIV; Immunologics; Individual; Infrastructure; Institution; Instruction; Investigation; Journals; Laboratories; Liver; Liver diseases; Maintenance; Methodology; Mission; Molecular; Molecular Target; Pathogenicity; Performance; Phase; Play; Protocols documentation; Reagent; Research; Research Personnel; Resources; Role; Services; Signal Pathway; Specimen; System; Techniques; Technology; Time; Tissues; Toxicology; Training and Education; Translational Research; United States National Institutes of Health; Universities; Whole Organism; career development; cost; cytokine; experience; experimental study; innovation; instrument; instrumentation; investigator training; liver injury; meetings; member; microscopic imaging; multidisciplinary; novel; programs; protocol development; research facility; square foot; success; toxicant

The University of Louisville Hepatobiology and Toxicology COBRE is a multidisciplinary group of investigators focusing on the liver and liver injury, gut:liver interactions, and liver:environment/toxicant/drug interactions. The Bioanalytical Core (Core C) is an integral part of the COBRE and will continue to play a key role in the implementation and future development of COBRE-funded projects. The Core provides the necessary expertise and state-of-the-art equipment for rigorous and comprehensive experimental evaluation of cell and tissue specimens to investigate the pathogenic mechanisms of liver disease. Besides providing requisite analytical services, a major goal of the Core C is to promote the education and training of the Center members, with particular attention to the COBRE junior investigators. Core C will specifically communicate with COBRE junior investigators regarding their experimental requirements on a continual basis and appropriately participate in the planning, execution and data interpretation. Core C will also interact with other COBRE Cores on a regular basis in order to avoid duplication of equipment or instrumentation and foster innovative protocol development and refinement, exchange of ideas, and integrative analysis of data. In addition to the upgraded existing equipment (BD FACSCanto II, Cellomics and Seahorse analyzers) Core C will also provide technical and analytical expertise with newly acquired, state-of-the-art, centralized equipment that includes BZ-X810 automated imaging microscope (Keyence), QuickPlex SQ 120 multiplex MSD analyzer and QuantStudio 6 Flex Real-Time PCR System. The Core facility is located in Clinical and Translational Research Building with 2,500 sq.ft of operating space that is contiguous with the 20,000 sq.ft. laboratory space of the Hepatobiology and Toxicology COBRE. Dr. Barve (Director) and the Core C research team have extensive experience in the development and execution of molecular, biochemical and immunological analyses that are critical for the operational and functional aspects of Core C. Dr. Barve was/is an investigator (PI, Co-PI or Co-I) on several NIH-funded grants, served for nearly a decade as the Director of the Molecular and Cytokine Core Laboratory of the NIH- funded GCRC, and supported the career development of several junior faculty. To remain a vital integrative component of the Center that provides services, not only for members but also the Institution as a whole, the Core C has the following specific aims: 1) To foster the development, sustainability and success of the proposed COBRE projects and pilots; 2) To play an active and significant role in the education and training of the investigators; 3) To assist and participate in the development of new experimental protocols; and techniques and 4) To become an integral and self-sustaining component of the COBRE Center.

路易斯维尔大学肝脏生物学和毒理学科布雷是一个多学科的研究小组 重点关注肝脏和肝脏损伤，肠道：肝脏相互作用，以及肝脏：环境/毒物/药物相互作用。 生物分析核心(C核心)是COBRE的一个组成部分，并将继续在 科布雷资助项目的实施和未来发展。核心提供了必要的 专业知识和最先进的设备，用于严格和全面的细胞和 组织标本，以探讨肝病的发病机制。除了提供必要的 分析服务，核心C的一个主要目标是促进中心成员的教育和培训， 特别关注科布雷初级调查员。Core C将专门与Cobre进行通信 初级调查人员对其实验要求的持续和适当的 参与规划、执行和数据解释。内核C还将与其他Cobre内核交互 定期进行，以避免重复使用设备或仪器，并促进创新的议定书 发展和提炼、思想交流和数据综合分析。除了升级后的 现有设备(BD FACSCanto II、细胞组学和海马分析仪)Core C也将提供技术支持 以及使用包括BZ-X810在内的新获得的最先进的集中式设备的分析专业知识 自动成像显微镜(Keyence)、QuickPlex SQ 120多路MSD分析仪和QuantStudio 6 Flex 实时荧光聚合酶链式反应系统。 核心设施位于临床和翻译研究大楼，运营面积为2500平方英尺 与20,000平方英尺相邻的空间。肝脏生物学和毒理学中心的实验室空间。 Barve博士(主任)和Core C研究团队在开发和 执行分子、生物化学和免疫学分析，这对操作和 核心C.的功能方面Barve博士曾经/现在是NIH资助的几个项目的研究人员(PI、Co-PI或Co-I 格兰特，担任美国国立卫生研究院分子和细胞因子核心实验室主任近十年- 资助GCRC，并支持几名初级教员的职业发展。保持重要的整合性 中心的组成部分，不仅为会员，而且为整个机构提供服务， 核心C有以下具体目标：1)促进发展、可持续性和成功 拟议的COBRE项目和试点；2)在以下方面发挥积极和重要的作用 研究人员；3)协助和参与制定新的实验方案；以及 技术和4)成为科布雷中心不可或缺的自给自足的组成部分。