Bioanalytical Core

路易斯维尔大学肝脏生物学和毒理学COBRE是一个多学科的研究小组 重点关注肝脏和肝脏损伤、肠道：肝脏相互作用和肝脏：环境/毒物/药物相互作用。 生物分析核心（核心C）是COBRE的组成部分，将继续在 实施和未来发展COBREF资助的项目。核心提供了必要的 专业知识和最先进的设备，对细胞进行严格和全面的实验评估， 组织标本，以研究肝脏疾病的致病机制。除了提供必要的 分析服务，核心C的主要目标是促进中心成员的教育和培训， 尤其是眼镜蛇行动的初级调查员核心C将专门与COBRE通信 初级研究人员持续适当地了解他们的实验要求 参与计划、执行和数据解释。核心C还将与其他COBRE核心进行交互 以避免设备或仪器的重复，并促进创新协议 发展和完善，交流思想，综合分析数据。除了升级的 现有设备（BD FACSCanto II、Cellomics和Seahorse分析仪）Core C也将提供技术支持。 和分析专业知识，包括BZ-X810在内的最先进的集中式设备 自动成像显微镜（Keyence）、QuickScan SQ 120多重MSD分析仪和QuantStudio 6 Flex 实时PCR系统。 核心设施位于临床和转化研究大楼， 与20，000平方英尺相邻的空间肝脏生物学和毒理学COBRE的实验室空间。 博士Barve（董事）和Core C研究团队在开发和 执行对操作和安全至关重要的分子、生物化学和免疫学分析， 核心C的功能方面。Barve博士是/是NIH资助的几项研究的研究者（PI，Co-PI或Co-I） 赠款，担任近十年的NIH分子和细胞因子核心实验室主任- 资助GCRC，并支持几个初级教师的职业发展。保持一个重要的综合性 中心的一个组成部分，不仅为会员，而且为整个机构提供服务， 核心C有以下具体目标：1）促进联合国系统的发展、可持续性和成功 （2）在教育和培训方面发挥积极和重要的作用， 3）协助和参与新实验方案的开发;以及 4）成为COBRE中心不可或缺的自我维持的组成部分。

The University of Louisville Hepatobiology and Toxicology COBRE is a multidisciplinary group of investigators focusing on the liver and liver injury, gut:liver interactions, and liver:environment/toxicant/drug interactions. The Bioanalytical Core (Core C) is an integral part of the COBRE and will continue to play a key role in the implementation and future development of COBRE-funded projects. The Core provides the necessary expertise and state-of-the-art equipment for rigorous and comprehensive experimental evaluation of cell and tissue specimens to investigate the pathogenic mechanisms of liver disease. Besides providing requisite analytical services, a major goal of the Core C is to promote the education and training of the Center members, with particular attention to the COBRE junior investigators. Core C will specifically communicate with COBRE junior investigators regarding their experimental requirements on a continual basis and appropriately participate in the planning, execution and data interpretation. Core C will also interact with other COBRE Cores on a regular basis in order to avoid duplication of equipment or instrumentation and foster innovative protocol development and refinement, exchange of ideas, and integrative analysis of data. In addition to the upgraded existing equipment (BD FACSCanto II, Cellomics and Seahorse analyzers) Core C will also provide technical and analytical expertise with newly acquired, state-of-the-art, centralized equipment that includes BZ-X810 automated imaging microscope (Keyence), QuickPlex SQ 120 multiplex MSD analyzer and QuantStudio 6 Flex Real-Time PCR System. The Core facility is located in Clinical and Translational Research Building with 2,500 sq.ft of operating space that is contiguous with the 20,000 sq.ft. laboratory space of the Hepatobiology and Toxicology COBRE. Dr. Barve (Director) and the Core C research team have extensive experience in the development and execution of molecular, biochemical and immunological analyses that are critical for the operational and functional aspects of Core C. Dr. Barve was/is an investigator (PI, Co-PI or Co-I) on several NIH-funded grants, served for nearly a decade as the Director of the Molecular and Cytokine Core Laboratory of the NIH- funded GCRC, and supported the career development of several junior faculty. To remain a vital integrative component of the Center that provides services, not only for members but also the Institution as a whole, the Core C has the following specific aims: 1) To foster the development, sustainability and success of the proposed COBRE projects and pilots; 2) To play an active and significant role in the education and training of the investigators; 3) To assist and participate in the development of new experimental protocols; and techniques and 4) To become an integral and self-sustaining component of the COBRE Center.