Functional Genomics & Microbiome Core

功能基因组学

• 批准号: 10377545
• 负责人: James Versalovic
• 金额: $ 19.11万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-15 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10377545
• 关键词: 16S ribosomal RNA sequencing; Affect; Biochemical; Bioinformatics; Biological; Biology; Biometry; Capital; Cells; Consultations; Coupling; DNA; DNA Sequence Alteration; Data; Data Analyses; Data Set; Detection; Digestive System Disorders; Epigenetic Process; Experimental Designs; Fostering; Gastrointestinal Injury; Gastrointestinal tract structure; Gene Expression; Gene Expression Profiling; Genes; Genomics; Goals; Hepatic; Human; Human Genome; Immune response; Infection; Injury; Instruction; Intestines; Investigation; Link; Liver; Liver diseases; Mass Spectrum Analysis; Medical center; Metabolic; Metagenomics; Microbe; Mission; Modeling; Molecular; Mus; Nucleic acid sequencing; Pancreas; Pancreatic Diseases; Pathway Analysis; Pathway interactions; Patients; Pattern; Problem Solving; Proteins; Proteomics; Research; Research Personnel; Resource Sharing; Resources; Services; Signal Pathway; Signal Transduction; Specific qualifier value; Specimen Handling; Spliced Genes; System; Systems Biology; Technology; Texas; Tissue Sample; Untranslated RNA; base; bisulfite sequencing; data analysis pipeline; data integration; established cell line; functional genomics; gastrointestinal; gastrointestinal function; gastrointestinal infection; genomic platform; informatics tool; innovation; insight; man; member; metabolomics; metagenome; metatranscriptomics; microbial; microbial composition; microbial genomics; microbiome; microbiome research; multiple omics; prevent; programs; protein metabolite; response; sequencing platform; tandem mass spectrometry; tool; transcriptome sequencing; transcriptomics

Project Summary The Functional Genomics and Microbiome Core (Core C) supports the mission of the DDC and enhances research programs in its theme of infection and injury affecting the mammalian intestine and liver. We continue to enhance the scope of the Core, based on DDC user demand and our firm commitment to the importance of the microbiome in gastrointestinal and liver biology. The emphasis of this Core is on functional genomics and how genes present in the mammalian microbiome and in mouse/human genomes may yield functional RNAs, proteins and metabolites that help stitch together a deeper and mechanistic understanding of digestive diseases. Our mission is to provide a full-service resource from experimental design to consultations about specimen processing, robust data analysis pipelines, bioinformatics strategies, and biostatistical support. In summary, we have created a fully integrated genomic and multi-`omic analysis platform for investigators studying digestive diseases. This Core serves DDC investigators by providing expertise in microbial and mammalian genomics, transcriptomics, metabolomics and protein analytics, as applied to gastrointestinal infection and injury states. Research programs nurtured by DDC members are facilitated by this Core's ability to bridge microbiome science and mammalian biology, and to provide multi-tiered services at the levels of genes and effectors (proteins and metabolites). This Core deploys the tools/services of microarrays, deep nucleic acid sequencing (microbial and mammalian), protein multiplexing, mass spectrometry-guided metabolomics, and bioinformatics. PCR-based analyses of gene expression and splicing, DNA mutation/SNP detection, and gene pathway analyses of the mammalian metagenome (microbe and man) will be fostered by this Core as a platform for gastrointestinal and hepatic systems biology. This Core presents a combination of shared as well as exclusive offerings to TMC DDC members. The Microbiome and Mammalian Genomics services provide valuable capital-intensive resources shared among DDC and non-DDC members at BCM. DDC members account for about 10% of institutional usage of metagenomics and mammalian genomics services. The Metabolomics/Mass Spectrometry, Protein Multiplexing, and Bioinformatics services are TMC DDC member-exclusive services, not offered to non-DDC members as Core functions.

项目概要 功能基因组学和微生物组核心（核心 C）支持 DDC 的使命和 加强影响哺乳动物肠道的感染和损伤主题的研究项目 肝。我们根据 DDC 用户需求和我们公司不断增强核心范围 致力于微生物组在胃肠道和肝脏生物学中的重要性。强调的是 该核心内容是功能基因组学以及基因如何存在于哺乳动物微生物组和 小鼠/人类基因组可能会产生有助于缝合在一起的功能性RNA、蛋白质和代谢物 对消化系统疾病有更深入和机械的了解。我们的使命是提供全方位服务 从实验设计到样本处理咨询、强大的数据分析的资源 管道、生物信息学策略和生物统计支持。总而言之，我们创建了一个完整的 为研究消化系统疾病的研究人员提供集成的基因组和多组学分析平台。这 Core 通过提供微生物和哺乳动物基因组学方面的专业知识为 DDC 研究人员提供服务， 转录组学、代谢组学和蛋白质分析，应用于胃肠道感染和损伤 州。该核心能力能够促进 DDC 成员培育的研究项目 微生物组科学和哺乳动物生物学，并提供基因水平的多层次服务 和效应物（蛋白质和代谢物）。该核心部署了微阵列的工具/服务，深入 核酸测序（微生物和哺乳动物）、蛋白质多重分析、质谱引导 代谢组学和生物信息学。基于 PCR 的基因表达和剪接分析、DNA 突变/SNP 检测以及哺乳动物宏基因组（微生物和 man）将由该核心培育为胃肠道和肝脏系统生物学的平台。这 Core 向 TMC DDC 成员提供共享和专有产品的组合。这 微生物组和哺乳动物基因组学服务提供宝贵的资本密集型资源共享 BCM 的 DDC 和非 DDC 成员。 DDC会员约占机构的10% 使用宏基因组学和哺乳动物基因组学服务。代谢组学/质谱分析， 蛋白质多重分析和生物信息学服务是 TMC DDC 会员专属服务，不提供 将非 DDC 成员作为核心职能。