Fluorescent probes for super-resolution imaging of the amyloid architecture

用于淀粉样蛋白结构超分辨率成像的荧光探针

• 批准号: DE210101176
• 负责人: Dr Amandeep Kaur
• 金额: $ 31.3万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Early Career Researcher Award
• 财政年份: 2021
• 资助国家: 澳大利亚
• 起止时间: 2021-06-30 至 2024-06-29
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DE210101176
• 关键词: Fluorescent; probes; super; resolution; imaging

The goal of this project is to develop chemical tools that enable molecular-level imaging of the amyloid structure. The Nobel Prize-winning super-resolution microscopy provides nanoscale imaging capabilities, but surprisingly there have been no substantive efforts to design fluorescent sensors that are compatible with this cutting-edge technology. In this project, new fluorescent super-resolution sensors will be developed that enable nanoscale visualisation of amyloid assemblies. These chemical and biochemical studies will establish rational design strategies to develop fluorescent sensors for super-resolution imaging applications and significantly advance our understanding of fundamental differences functional and toxic protein assemblies.

该项目的目标是开发化学工具，使淀粉样蛋白结构的分子水平成像。获得诺贝尔奖的超分辨率显微镜提供了纳米级的成像能力，但令人惊讶的是，没有实质性的努力来设计与这种尖端技术兼容的荧光传感器。在这个项目中，将开发新的荧光超分辨率传感器，使淀粉样蛋白组装的纳米级可视化。这些化学和生物化学研究将建立合理的设计策略，以开发用于超分辨率成像应用的荧光传感器，并显着推进我们对功能和毒性蛋白质组装体的根本差异的理解。