Next generation cell imaging: developing new genetically encodable super-scattering Raman probes

下一代细胞成像：开发新型基因可编码超散射拉曼探针

• 批准号: 2887769
• 金额: --
• 依托单位: CARDIFF UNIVERSITY
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2887769
• 关键词: Next; generation; cell; imaging; developing

Advances in cellular bioimaging have proved pivotal in improving our understanding of biological processes. Fluorescence microscopy emerged as the "go to" cell imaging approach as fluorescent proteins (FPs) allowed genetic tagging and tracking of specific targets inside a cell. However, there are disadvantages such as photobleaching and the limited number of targets that can be monitored simultaneously. Raman microscopy has the potential to overcome these limitations since Raman scattering can be spectrally narrow and tuneable, as well as photostable, but requires the development of genetically encoded probes.

细胞生物成像的进展已被证明是提高我们对生物过程理解的关键。荧光显微镜作为“去”细胞成像方法出现，因为荧光蛋白（FP）允许遗传标记和跟踪细胞内的特定靶点。然而，也有缺点，如光漂白和有限数量的目标，可以同时监测。拉曼显微镜具有克服这些限制的潜力，因为拉曼散射可以是光谱窄的和可调谐的，以及光稳定的，但需要开发基因编码的探针。