Core B: Tissue Specimen Core

核心 B：组织样本核心

• 批准号: 10384401
• 负责人: Stephanie Halene
• 金额: $ 35.44万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-30 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10384401
• 关键词: Core; Tissue; Specimen

PROJECT SUMMARY – Core B (Biospecimen Core) Primary (bone marrow and thymus) and secondary (tonsil, lymph node, spleen and others) lymphoid organs are among the most dynamic tissues throughout an individual’s lifetime. The bone marrow gives rise to hundreds of billions of cells each day, the thymus educates T-cells to recognize self and non-self and tonsils, lymph nodes, spleen and others are key to daily defense against invading organisms and maintenance of cellular homeostasis. Cellular senescence in these lymphoid tissues have been observed and presumably associated with stress-induced or age-related functional impairment. However, what types or subtypes of senescent cells are present and their spatial heterogeneity in in the tissue context as well as how these cells impact the tissue environments remain poorly understood, highlighting a pressing need to map senescent cells and the surround environments in these tissues and generate biomarkers to define spatial and phenotypic heterogeneity in cellular senescence. The Biospecimen Core will fulfill a critical role in the Yale SenNet TMC to procure, process, and histologically annotate lymphoid tissue specimens for further analysis in the Biological Analysis Core to generate molecular and cellular maps of senescent cells. It will leverage and significantly expand upon the Yale Hematology Tissue Bank’s existing structure and further leverage the existing collaborations between Hematology (Dr. Halene), Pathology (Dr. Xu), Immunobiology (Dr. Craft), Laboratory Medicine and YNHH Health Data Science (Dr. Schulz), and Surgery (Dr. Kodadek) to collect lymphoid tissue and concurrent and longitudinal multi-specimen blood samples from healthy individuals. The Biospecimen Core will collect, process, aliquot, catalog, annotate high quality tissue samples and distribute these to the Yale SenNET Biological Analysis Core and SenNET investigators. The Biospecimen Core will leverage its existing Freezerworks database to integrate all data from patient to tissue aliquot and link this data to clinical and research databases in a HIPAA compliant and auditable fashion. The tissue specimens including tissue sections and biofluids are further analyzed by the Biological Analysis Core and the specimen metadata will be managed in the Data Analysis Core for integrated analysis and sharing.

项目总结-核心B（生物样本核心） 初级（骨髓和胸腺）和次级（扁桃体、淋巴结、脾脏和其他）淋巴器官 是人一生中最活跃的组织之一骨髓会产生 每天有数千亿个细胞，胸腺训练T细胞识别自我和非自我， 淋巴结，脾脏和其他是关键的日常防御入侵的生物体和维护 细胞内稳态已经观察到这些淋巴组织中的细胞衰老， 与压力引起的或年龄相关的功能障碍有关。然而，什么类型或亚型的 衰老细胞的存在及其在组织环境中的空间异质性以及这些细胞如何 对组织环境的影响仍然知之甚少，突出了绘制衰老细胞图谱的迫切需要 以及这些组织中的周围环境，并产生生物标志物来定义空间和表型 细胞衰老的异质性。生物样本核心将在耶鲁大学SenNet TMC中发挥关键作用， 采集、处理和组织学注释淋巴组织标本，以便在生物学实验室中进行进一步分析。 分析核心生成衰老细胞的分子和细胞图谱。它将发挥杠杆作用， 扩展耶鲁血液学组织库的现有结构，并进一步利用现有的 血液学（Halene博士）、病理学（徐博士）、免疫生物学（Craft博士）、实验室 医学和YNHH健康数据科学（Schulz博士）和外科（Kodadek博士）收集淋巴组织 以及来自健康个体的同时和纵向多样本血液样品。生物标本 核心将收集，处理，等分，目录，注释高质量的组织样本，并分发给耶鲁大学 SenNET生物分析核心和SenNET研究者。生物标本核心将利用其现有的 Freezerworks数据库，以整合从患者到组织等分试样的所有数据，并将该数据与临床和 研究数据库符合HIPAA和可审计的方式。组织标本包括组织 切片和生物流体由生物分析核心进一步分析，标本元数据将被 在数据分析核心中进行管理，以实现集成分析和共享。