Core B: Tissue Specimen Core

核心 B：组织样本核心

• 批准号: 10384401
• 负责人: Stephanie Halene
• 金额: $ 35.44万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-30 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10384401
• 关键词: Core; Tissue; Specimen

PROJECT SUMMARY – Core B (Biospecimen Core) Primary (bone marrow and thymus) and secondary (tonsil, lymph node, spleen and others) lymphoid organs are among the most dynamic tissues throughout an individual’s lifetime. The bone marrow gives rise to hundreds of billions of cells each day, the thymus educates T-cells to recognize self and non-self and tonsils, lymph nodes, spleen and others are key to daily defense against invading organisms and maintenance of cellular homeostasis. Cellular senescence in these lymphoid tissues have been observed and presumably associated with stress-induced or age-related functional impairment. However, what types or subtypes of senescent cells are present and their spatial heterogeneity in in the tissue context as well as how these cells impact the tissue environments remain poorly understood, highlighting a pressing need to map senescent cells and the surround environments in these tissues and generate biomarkers to define spatial and phenotypic heterogeneity in cellular senescence. The Biospecimen Core will fulfill a critical role in the Yale SenNet TMC to procure, process, and histologically annotate lymphoid tissue specimens for further analysis in the Biological Analysis Core to generate molecular and cellular maps of senescent cells. It will leverage and significantly expand upon the Yale Hematology Tissue Bank’s existing structure and further leverage the existing collaborations between Hematology (Dr. Halene), Pathology (Dr. Xu), Immunobiology (Dr. Craft), Laboratory Medicine and YNHH Health Data Science (Dr. Schulz), and Surgery (Dr. Kodadek) to collect lymphoid tissue and concurrent and longitudinal multi-specimen blood samples from healthy individuals. The Biospecimen Core will collect, process, aliquot, catalog, annotate high quality tissue samples and distribute these to the Yale SenNET Biological Analysis Core and SenNET investigators. The Biospecimen Core will leverage its existing Freezerworks database to integrate all data from patient to tissue aliquot and link this data to clinical and research databases in a HIPAA compliant and auditable fashion. The tissue specimens including tissue sections and biofluids are further analyzed by the Biological Analysis Core and the specimen metadata will be managed in the Data Analysis Core for integrated analysis and sharing.

项目摘要 - 核心B（双元素核心） 原发性（骨髓和胸腺）和次级（扁桃体，淋巴结，脾脏等）淋巴器官 是整个人一生中最动态的组织之一。骨髓产生 每天数百亿个细胞，胸腺教育T细胞识别自我，非自我和扁桃体， 淋巴结，脾和其他人是日常防御入侵生物和维护的关键 细胞稳态。已经观察到这些淋巴组织中的细胞感应，并且大概已经观察到 与压力诱导或与年龄相关的功能障碍有关。但是，什么类型或亚型 有感觉细胞存在，它们在组织环境中的空间异质性以及这些细胞如何 影响组织环境的理解仍然很少，突出了绘制感觉细胞的紧迫需求 以及这些组织中的周围环境，并产生生物标志物来定义空间和表型 细胞感应中的异质性。 Biospecimen Core将在耶鲁Sennet TMC中扮演关键角色 采购，过程和组织学注释淋巴组织标本，以在生物学中进行进一步分析 分析核心生成感觉细胞的分子图和细胞图。它将充分利用 扩展耶鲁大学血液学组织现有结构，并进一步利用现有的 血液学（Halene博士），病理学（XU博士），免疫生物学（Craft博士），实验室的合作 医学和YNHH健康数据科学（Schulz博士）和手术（Kodadek博士）收集淋巴组织 以及来自健康个体的并发和纵向多种血液样本。生物测量 核心将收集，过程，等分试样，目录，注释高质量的组织样本并将其分配给耶鲁 Sennet生物分析核心和Sennet研究人员。 BiospeCemen Core将利用其现有的 Freezerworks数据库以整合从患者到组织等分试样的所有数据，并将这些数据与临床和 以HIPAA为符合HIPAA的研究数据库。包括组织在内的组织标本 通过生物分析核心进一步分析了部分和生物流体，标本元数据将是 在数据分析核心中进行管理，用于集成分析和共享。