Core B: Tissue Specimen Core

项目摘要--核心B(生物质学家核心) 初级(骨髓和胸腺)和次级(扁桃体、淋巴结、脾等)淋巴器官 是人一生中最有活力的组织之一。骨髓产生了 胸腺每天有数千亿个细胞，培养T细胞识别自我和非我以及扁桃体， 淋巴、脾和其他器官是日常防御入侵生物和维持 细胞动态平衡。已经观察到这些淋巴组织中的细胞衰老，并推测 与压力引起的或与年龄相关的功能障碍有关。但是，哪些类型或子类型 衰老细胞的存在及其在组织中的空间异质性以及这些细胞是如何 对组织环境的影响仍然知之甚少，突显了绘制衰老细胞图谱的迫切需要 以及这些组织中的周围环境，并产生生物标记物来定义空间和表型 细胞衰老的异质性。Biosecimen核心将在耶鲁Sennet TMC中发挥关键作用 获取、加工和组织学注释淋巴组织标本，以便在《生物学》杂志中进一步分析 分析核心，以生成衰老细胞的分子和细胞图。它将发挥杠杆作用并显著 扩展耶鲁大学血液组织库的现有结构，并进一步利用现有的 血液学(Halene博士)、病理学(Xu博士)、免疫生物学(Craft博士)、实验室之间的合作 医学和基督教青年会健康数据科学(舒尔茨博士)和外科(柯达德克博士)收集淋巴组织 以及来自健康个体的并行和纵向多样本血液样本。生物菌素 CORE将收集、处理、等分、编目、注释高质量的组织样本，并将这些样本分发给耶鲁大学 Sennet生物分析核心和Sennet调查人员。Biospecimen核心将利用其现有的 可集成从患者到组织等分的所有数据，并将这些数据链接到临床和 以符合HIPAA和可审计的方式研究数据库。组织标本包括组织 切片和生物流体将由生物分析核心进一步分析，标本元数据将 在数据分析核心中进行管理，以进行集成分析和共享。

PROJECT SUMMARY – Core B (Biospecimen Core) Primary (bone marrow and thymus) and secondary (tonsil, lymph node, spleen and others) lymphoid organs are among the most dynamic tissues throughout an individual’s lifetime. The bone marrow gives rise to hundreds of billions of cells each day, the thymus educates T-cells to recognize self and non-self and tonsils, lymph nodes, spleen and others are key to daily defense against invading organisms and maintenance of cellular homeostasis. Cellular senescence in these lymphoid tissues have been observed and presumably associated with stress-induced or age-related functional impairment. However, what types or subtypes of senescent cells are present and their spatial heterogeneity in in the tissue context as well as how these cells impact the tissue environments remain poorly understood, highlighting a pressing need to map senescent cells and the surround environments in these tissues and generate biomarkers to define spatial and phenotypic heterogeneity in cellular senescence. The Biospecimen Core will fulfill a critical role in the Yale SenNet TMC to procure, process, and histologically annotate lymphoid tissue specimens for further analysis in the Biological Analysis Core to generate molecular and cellular maps of senescent cells. It will leverage and significantly expand upon the Yale Hematology Tissue Bank’s existing structure and further leverage the existing collaborations between Hematology (Dr. Halene), Pathology (Dr. Xu), Immunobiology (Dr. Craft), Laboratory Medicine and YNHH Health Data Science (Dr. Schulz), and Surgery (Dr. Kodadek) to collect lymphoid tissue and concurrent and longitudinal multi-specimen blood samples from healthy individuals. The Biospecimen Core will collect, process, aliquot, catalog, annotate high quality tissue samples and distribute these to the Yale SenNET Biological Analysis Core and SenNET investigators. The Biospecimen Core will leverage its existing Freezerworks database to integrate all data from patient to tissue aliquot and link this data to clinical and research databases in a HIPAA compliant and auditable fashion. The tissue specimens including tissue sections and biofluids are further analyzed by the Biological Analysis Core and the specimen metadata will be managed in the Data Analysis Core for integrated analysis and sharing.