Genomics-Bioinformatics Core

项目总结 在全球和单个细胞水平上探测基因表达和染色质景观的能力 打开大门，寻求前所未有的信息和洞察发展、动态平衡、修复和 皮肤作为一个复杂器官的发病机制。加州大学欧文分校拥有超过临界数量的皮肤生物学家 建立旨在了解潜在的监管机制的研究专业知识和计划 皮肤中的表皮、真皮、色素和血管细胞的生物学，以及这些机制如何在 牛皮癣和皮肤癌等疾病。虽然这些皮肤生物学家都能接触到普通基因组学 校园现有的技术，迫切需要适应和发展独特和创新的技术 为皮肤生物学量身定做的技术和服务，以加快科学发现的进程。 基因组学和生物信息学核心的目标是成为皮肤中心的关键资源组成部分 将其成员研究人员置于新基因组学和生物信息学发展的前沿。这个 CORE将利用现有的校园资源，如技术仪器以及基因组学和 在教职员工和研究人员层面的生物信息学专业知识，以开发新的针对皮肤的服务并提供 为中心实验室提供技术和智力支持。核心的第一个具体目标是提供状态- 用于基因表达和表观基因组图谱的最先进的基因组学技术(例如，Illumina RNA-seq，NanoString. 计数器、ATAC-SEQ和CHIP-SEQ)以及专门的员工时间和相关的统计分析(使用 商业和定制管道)，以推进皮肤研究。第二个具体目标是促进 单细胞分析方面的创新，以促进对细胞异质性、分化层次、 以及皮肤中的细胞-细胞相互作用，例如通过测试和应用新的仪器和技术 单细胞操作，为单细胞数据开发新的谱系预测生物信息学工具，以及 建立和改进多重原位RNA检测以建立基因表达和细胞的空间图谱 皮肤组织中的类型或细胞状态。第三个具体目标是维护一个存储库，其中包含所有由中心生成 皮肤测序数据并创建公众可访问的数据库，例如包含单细胞的新网站 具有时间和空间信息的数据，可供广大皮肤研究社区使用。最后 具体目标是提供有组织的活动(如研讨会、讲习班、迷你课程)以及一对一 就基础基因组学和生物信息学对中心皮肤生物学家进行培训和教育。总而言之，这些 AIMS将对加州大学欧文分校的皮肤研究产生重大而持久的影响，不仅促进重要的 科学发现，也培养了精通基因组学和 生物信息学。

PROJECT SUMMARY The ability to probe gene expression and chromatin landscape globally and at a single cell level has opened the door to seek unprecedented information and insight into the development, homeostasis, repair, and pathogenesis of skin as a complex organ. UC Irvine enjoys more than a critical mass of skin biologists with well- established research expertise and programs that aim at understanding the regulatory mechanisms underlying the biology of epidermal, dermal, pigment and vascular cells in skin, and how such mechanisms may go awry in diseases such as psoriasis and skin cancer. While these skin biologists all have access to general genomics techniques currently available on campus, there is an urgent need to adapt and develop unique and innovative techniques and services that are tailored to skin biology in order to speed up the process of scientific discoveries. The goal of the Genomics and Bioinformatics Core is to serve as a critical resource component of the Skin Center to place its member investigators at the cutting edge of new genomics and bioinformatics developments. The Core will leverage existing campus resources, such as technological instrumentation as well as genomics and bioinformatics expertise at both faculty and researcher levels, to develop new skin-specific services and provide technical and intellectual support to Center laboratories. The first specific aim of the Core is to provide state-of- the-art genomics technologies for gene expression and epigenome profiling (e.g., Illumina RNA-seq, NanoString nCounter, ATAC-seq, and ChIP-seq) along with dedicated staff time and associated statistical analyses (using both commercial and customized pipelines) to advance skin research. The second specific aim is to promote innovation in single cell analysis to foster understanding of cellular heterogeneities, differentiation hierarchies, and cell-cell interactions in skin, for example by testing and applying new instrumentation and technologies for single cell manipulation, developing new lineage-predicting bioinformatics tools for single cell data, as well as establishing and improving multiplexed in situ RNA detection to create spatial maps of gene expression and cell types or cellular states in the skin tissue. The third specific aim is to maintain a repository of all Center-generated skin sequencing data and create publically accessible databases, such as a new website containing single cell data with temporal and spatial information that is available to the skin research community at large. The last specific aim is to provide organized activities (e.g., seminars, workshops, mini-courses) as well as one-on-one consultation to train and educate Center skin biologists on basic genomics and bioinformatics. Collectively, these aims will make significant and long-lasting impact on skin research at UC Irvine to not only facilitate important scientific discoveries but also cultivate next-generation skin scientists with proficiency in genomics and bioinformatics.