HOST INTERACTIONS OF THE YEAST RETROTRANSPOSON TY3

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Ty 3产生类似于逆转录病毒核心颗粒的病毒样颗粒。 然而，在Ty 3的情况下，结构蛋白开放阅读框中约60个Ala扫描突变可用于研究结构蛋白与其自身以及与宿主蛋白的相互作用。 我们已经在两个杂交诱饵和激活载体中构建了Gag 3插入，其靶向Gal 4介导的控制。 结构蛋白（Gag 3）已被证明与自身相互作用，但在某些突变体中受到影响。 其中两个突变是影响Gag 3和宿主蛋白之间相互作用的候选者。初步数据表明，这些蛋白质可能参与囊泡运输和P体定位。我们建议筛选酵母基因组文库，以测试与Ty 3 Gag 3相互作用并参与颗粒组装的宿主蛋白。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ty3 produces virus-like particles similar to the core particles of retroviruses. In the case of Ty3 however, about 60 Ala scanning mutations in the structural protein open reading frame are available with which to study interactions of the structural protein with itself and with host proteins. We have constructed Gag3 insertions in two hybrid bait and activation vectors which target Gal4-mediated control. The structural protein (Gag3) has been shown to interact with itself but to be affected in certain mutants. Two of the mutations are candidates for affecting interactions between Gag3 and host proteins. Preliminary data suggests that these proteins might be involved in vesicular trafficking and P-body localization. We propose to screen a yeast genomic library in order to test for host proteins that interact with Ty3 Gag3 and are involved in particle assembly.