High Throughput DNA Sequencer

高通量 DNA 测序仪

• 批准号: 7594911
• 负责人: SUZANNE SANDMEYER
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7594911
• 关键词: Administrator; Area; Bacterial Genome; Biomedical Research; Code; Commit; Core Facility; DNA; DNA Resequencing; DNA Sequence; Data; Disease; Disease susceptibility; Epigenetic Process; Equipment; Experimental Designs; Funding; Gene Expression; Genetic Transcription; Genetic Variation; Genome; Genomics; Human; Human Genome; Knowledge; Malignant Neoplasms; Malignant neoplasm of lung; Modeling; Operative Surgical Procedures; Protein Binding; Protein Microchips; Proteins; RNA Splicing; Regulation; Research; Research Personnel; Research Training; Sheep; Site; System; Technology; Time; Untranslated RNA; Variant; Virulence; genetic regulatory protein; genome sequencing; genome wide association study; genome-wide; genome-wide analysis; instrument; public health relevance; tool

DESCRIPTION (provided by applicant): We propose to purchase a Solexa Genome Analyzer System from Illumina. High throughput sequence data will positively impact campus research. Twenty-one investigators will be enabled to perform projects in the areas of: 1) genome resequencing to identify bacterial virulence determinants and to correlate human gene variants with disease susceptibility; 2) interaction of proteins with chromosomal DNA throughout the genome in order to understand transcriptional and epigenetic regulation; and 3) genomewide gene expression to enable understanding of the comprehensive picture of transcription from coding sequences to noncoding RNAs. High throughput DNA sequence technologies have two very significant advantages over current technologies. First, data are collected so efficiently that fundamental changes in experimental design are enabled, for example by allowing rapid resequencing of bacterial genomes for variations association with virulence or resequencing segment of the human genome for SNP association studies. Second, most applications of sequencing are less dependent on what is already known than microarrays, the other major genome wide analysis tool which is completely dependent upon knowledge of target genome sequence or in the case of some gene expression arrays, also dependent on knowledge about what is transcribed and even what is spliced. The sequencer will be added to the current DNA and Protein MicroArray Facility to form a UCI Genomics Core Facility. Funds have been committed by the campus to complete purchase of the Solexa instrument and extended warranties and purchase the necessary accessory equipment, and to hire a full time technician and part time systems administrator to staff this expansion. Acquisition of the sequencer will have a high positive impact on research and training at this rapidly growing UC campus. PUBLIC HEALTH RELEVANCE: A consortium of investigators from across campus proposes to acquire a Solexa high throughput sequencer from Illumina for the UCI Genomics Core Facility. High throughput sequencing dramatically increases the amount of sequence which can be collected in one experimental operation. Addition of this instrument to the Core Facility will enable exciting biomedical research. Projects include sequencing entire bacterial genomes to discover virulence determinants, analysis of human genomes to identify sequence variants associated with disease, identification of sites across the human genome where cancer-related regulatory proteins bind to control gene expression, and quantification of changes in gene expression in a sheep model for lung cancer.

描述（由申请人提供）：我们提议从Illumina购买Solexa基因组分析仪系统。高通量序列数据将对校园研究产生积极影响。21名研究人员将能够执行以下领域的项目：1）基因组重新测序，以确定细菌毒力决定因素，并将人类基因变异与疾病易感性联系起来; 2）蛋白质与整个基因组中染色体DNA的相互作用，以了解转录和表观遗传调控;和3）全基因组基因表达，从而能够理解从编码序列到非编码RNA的转录的全面画面。高通量DNA测序技术与现有技术相比具有两个非常显著的优点。首先，如此有效地收集数据，使得能够实现实验设计中的根本改变，例如通过允许细菌基因组的快速重测序以用于与毒力相关的变异或人类基因组的重测序区段以用于SNP关联研究。第二，测序的大多数应用与微阵列相比较少依赖于已知的内容，微阵列是另一种主要的全基因组分析工具，其完全依赖于靶基因组序列的知识，或者在某些基因表达阵列的情况下，还依赖于关于转录什么甚至剪接什么的知识。测序仪将被添加到目前的DNA和蛋白质微阵列设施，形成UCI基因组学核心设施。校园已承诺提供资金，以完成购买Solexa仪器和延长保修期，并购买必要的附属设备，并聘请一名全职技术人员和兼职系统管理员，以工作人员这一扩张。测序仪的收购将对这个快速发展的加州大学校园的研究和培训产生高度积极的影响。 公共卫生相关性：一个来自校园的研究者联盟提议从Illumina为UCI基因组核心设施购买Solexa高通量测序仪。高通量测序显著地增加了可以在一个实验操作中收集的序列的量。将该仪器添加到核心设施将使令人兴奋的生物医学研究成为可能。项目包括对整个细菌基因组进行测序以发现毒力决定因素，分析人类基因组以确定与疾病相关的序列变异，确定人类基因组中癌症相关调节蛋白结合控制基因表达的位点，以及量化绵羊肺癌模型中基因表达的变化。