Systematic Discovery and Analysis of Small Proteins and Small ORFs in Mycobacteria
Systematic Discovery and Analysis of Small Proteins and Small ORFs in Mycobacteria
批准号:
10388045
负责人:
KEITH M DERBYSHIRE
金额:
$0.89万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2020
资助国家:
美国
项目状态:
已结题
起止时间:
2020-08-01 至 2024-07-31
关键词:
AlgorithmsAmino AcidsBasic ScienceBiochemicalBirthCell ExtractsCysteineEukaryotaFoxesFriendsFutureGenesGenomeGenomicsGenus MycobacteriumGoalsHomeostasisLaboratory ResearchLearningMass Spectrum AnalysisMediatingMentorsMolecular BiologyMutationOpen Reading FramesPeptidesPreparationProkaryotic CellsPropertyProteinsProteomicsRegulationResearchScienceScientistTrainingWorkcareerexperiencegenome annotationmutantskillssummer researchundergraduate research experienceundergraduate studentwhole genome
中文摘要
过去几十年见证了分子生物学领域的诞生和成熟。最初,突变
基因是基因组探索的焦点。现在,整个基因组都被例行测序,
通过注释算法自动识别常驻基因。另外,蛋白质组学方法
制备用于质谱分析的全细胞提取物的蛋白水解肽。这一切成功都
方法对大基因有强烈的偏见:大基因是突变的常见目标,长期开放,
阅读框在基因组序列中很容易识别,大的蛋白质产生许多肽
用于质谱鉴定。这种无意的偏见也造成了我们的巨大差距,
了解分子生物学。
最近在真核生物和原核生物中的研究发现了大量的小基因或其
编码蛋白质。小分子蛋白质(被认为是50个氨基酸或更少)的数量与传统的
大的蛋白质,但只有少数已被归因于一个功能。该提案的目的是推动
这一新兴领域的发展,促进了小蛋白的发现和功能表征。我们
将在分枝杆菌中开发和应用我们的小蛋白方法。分枝杆菌提供许多
小蛋白研究的优势。最重要的是,它们在标准蛋白质中表达>1000种小蛋白质。
条件
这个补充的目标是支持一个优秀的本科生,劳伦福克斯,谁渴望
成为一名研究科学家,拥有直接的实验室研究经验。劳伦会专注于
剖析半胱氨酸中一组独特的s蛋白介导的调节机制
体内平衡她将学习新的技术和分析技能,并更好地了解
成为一名研究科学家需要什么参与沃兹沃斯夏季研究计划,
本科生将进一步暴露劳伦在科学,未来的导师和新朋友的潜在职业
和同事。
英文摘要
The last few decades have seen the birth and maturation of the field of Molecular Biology. Initially, mutant
genes were focal points of genome exploration. Now, entire genomes are routinely sequenced, and the
resident genes are automatically identified by annotation algorithms. Alternatively, proteomic approaches
prepare proteolytic peptides of whole-cell extracts for analysis by mass spectrometry. Each of these
approaches are strongly biased for large genes: large genes are frequent targets for mutation, long-open-
reading frames are easily discerned in genomic sequence, and large proteins generate many peptides
for mass spectrometry identification. This unintended bias has also created a large gap in our
understanding of molecular biology.
Recent work in eukaryotes and prokaryotes alike have uncovered multitudes of small genes or their
encoded proteins. The numbers of small proteins (considered as 50 aa or less) rival that of traditionally
large proteins, yet only a handful have been ascribed a function. The goal of this proposal is to propel
this nascent field forward by facilitating both small protein discovery and functional characterization. We
will develop and apply our small protein approaches in mycobacteria. Mycobacteria offer many
advantages for small protein study. Foremost is that they express >1000 small proteins in standard
conditions.
The goal of this supplement is to support an outstanding undergraduate student, Lauren Fox, who aspires
to be a research scientist, with direct hands-on laboratory research experience. Lauren will focus on
dissecting the mechanism of regulation mediated by a unique set of sproteins involved in cysteine
homeostasis. She will learn new technical and analytical skills, and develop a greater understanding of
what it takes to be a research scientist. Involvement in the Wadsworth summer research program for
undergraduates will further expose Lauren to potential careers in science, future mentors and new friends
and colleagues.
期刊论文(0)
专著(0)
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会议论文
Dissecting and connecting the SigM stimulus and ESX-4 secretory response in mycobacteria
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批准号:10339992
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项目类别:
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资助金额:$36.57万
-
财政年份:2022
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负责人:KEITH M DERBYSHIRE
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依托单位:
Dissecting and connecting the SigM stimulus and ESX-4 secretory response in mycobacteria
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批准号:10706956
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项目类别:
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资助金额:$40.53万
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财政年份:2022
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负责人:KEITH M DERBYSHIRE
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依托单位:
Systematic Discovery and Analysis of Small Proteins and Small ORFs in Mycobacteria
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批准号:10221007
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项目类别:
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资助金额:$56.19万
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财政年份:2020
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负责人:KEITH M DERBYSHIRE
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依托单位:
Systematic Discovery and Analysis of Small Proteins and Small ORFs in Mycobacteria
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批准号:10663206
-
项目类别:
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资助金额:$54.7万
-
财政年份:2020
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负责人:KEITH M DERBYSHIRE
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依托单位:
Systematic Discovery and Analysis of Small Proteins and Small ORFs in Mycobacteria
-
批准号:10452528
-
项目类别:
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资助金额:$54.7万
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财政年份:2020
-
负责人:KEITH M DERBYSHIRE
-
依托单位:
Characterization of the Abundant Small Proteome of Mycobacteria
-
批准号:8949153
-
项目类别:
-
资助金额:$23.62万
-
财政年份:2015
-
负责人:KEITH M DERBYSHIRE
-
依托单位:
Empirically Defining Gene Architecture and Expression of M. Tuberculosis
-
批准号:8868643
-
项目类别:
-
资助金额:$18.14万
-
财政年份:2015
-
负责人:KEITH M DERBYSHIRE
-
依托单位:
Characterization of the Abundant Small Proteome of Mycobacteria
-
批准号:9090002
-
项目类别:
-
资助金额:$19.43万
-
财政年份:2015
-
负责人:KEITH M DERBYSHIRE
-
依托单位:
Genome Scale Discovery of Mycobacterial Gene Function by Synthetic Genetic Arrays
-
批准号:8567025
-
项目类别:
-
资助金额:$15.74万
-
财政年份:2013
-
负责人:KEITH M DERBYSHIRE
-
依托单位:
Genome Scale Discovery of Mycobacterial Gene Function by Synthetic Genetic Arrays
-
批准号:8664347
-
项目类别:
-
资助金额:$19.44万
-
财政年份:2013
-
负责人:KEITH M DERBYSHIRE
-
依托单位:
A Community Mycobacterial Systems Resource
-
批准号:8369895
-
项目类别:
-
资助金额:$56.52万
-
财政年份:2012
-
负责人:KEITH M DERBYSHIRE
-
依托单位:
A Community Mycobacterial Systems Resource
-
批准号:8467675
-
项目类别:
-
资助金额:$58.61万
-
财政年份:2012
-
负责人:KEITH M DERBYSHIRE
-
依托单位:
A Community Mycobacterial Systems Resource
-
批准号:8653529
-
项目类别:
-
资助金额:$70.29万
-
财政年份:2012
-
负责人:KEITH M DERBYSHIRE
-
依托单位:
Localization and assembly of the M. tuberculosis ESX-1 secretory apparatus
-
批准号:8020090
-
项目类别:
-
资助金额:$21.93万
-
财政年份:2010
-
负责人:KEITH M DERBYSHIRE
-
依托单位:
Localization and assembly of the M. tuberculosis ESX-1 secretory apparatus
-
批准号:7871120
-
项目类别:
-
资助金额:$18.63万
-
财政年份:2010
-
负责人:KEITH M DERBYSHIRE
-
依托单位:
Molecular Dissection of Conjugation, Virulence and ESX Secretion in Mycobacteria
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批准号:8079913
-
项目类别:
-
资助金额:$46.12万
-
财政年份:2010
-
负责人:KEITH M DERBYSHIRE
-
依托单位:
Molecular Dissection of Conjugation, Virulence and ESX Secretion in Mycobacteria
-
批准号:7865197
-
项目类别:
-
资助金额:$45.39万
-
财政年份:2009
-
负责人:KEITH M DERBYSHIRE
-
依托单位:
Conjugation and recombination in mycobacteria
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批准号:7846531
-
项目类别:
-
资助金额:$1.05万
-
财政年份:2009
-
负责人:KEITH M DERBYSHIRE
-
依托单位:
Congugal DNA transfer into M. tuberculosis
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批准号:7244135
-
项目类别:
-
资助金额:$20.92万
-
财政年份:2006
-
负责人:KEITH M DERBYSHIRE
-
依托单位:
Congugal DNA transfer into M. tuberculosis
-
批准号:7129156
-
项目类别:
-
资助金额:$18.62万
-
财政年份:2006
-
负责人:KEITH M DERBYSHIRE
-
依托单位:
海外基金