Systematic Discovery and Analysis of Small Proteins and Small ORFs in Mycobacteria
分枝杆菌中小蛋白和小 ORF 的系统发现和分析
基本信息
- 批准号:10452528
- 负责人:
- 金额:$ 54.7万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-08-01 至 2024-07-31
- 项目状态:已结题
- 来源:
- 关键词:5&apos Untranslated RegionsAlgorithmsAmino AcidsArchaeaArchitectureBacteriaBiochemicalBioinformaticsBiologyBirthCell ExtractsCodon NucleotidesCollaborationsCouplingCryoelectron MicroscopyCysteineDataData SetDetectionEukaryotaFruitGene ProteinsGenesGenomeGenomicsGenus MycobacteriumGoalsInfectionInstructionKnowledgeLaboratoriesLifeMass Spectrum AnalysisMedicalMessenger RNAMethodsMolecular BiologyMolecular GeneticsMutationOpen Reading FramesOperonOrganismPathogenesisPathogenicityPeptidesPreparationPrevalenceProkaryotic CellsPropertyProteinsProteomicsRegulationResearch PersonnelRibosomal ProteinsRibosomesRoleSelection BiasSignal TransductionStructureTestingTrainingTranslatingTranslationsWorkattenuationcomparativeexperimental studygenetic approachgenome annotationgenome-wideimprovedinsightmembermutantmycobacterialnovelprotein functionresponseribosome profilingwhole genome
项目摘要
The last few decades have seen the birth and maturation of the field of Molecular Biology. Initially, mutant
genes were focal points of genome exploration. Now, entire genomes are routinely sequenced, and the
resident genes are automatically identified by annotation algorithms. Alternatively, proteomic approaches
prepare proteolytic peptides of whole-cell extracts for analysis by mass spectrometry. Each of these
approaches are strongly biased for large genes: large genes are frequent targets for mutation, long-open-
reading frames are easily discerned in genomic sequence, and large proteins generate many peptides
for mass spectrometry identification. This unintended bias has also created a large gap in our
understanding of molecular biology.
Recent work in eukaryotes and prokaryotes alike have uncovered multitudes of small genes or their
encoded proteins. The numbers of small proteins (considered as 50 aa or less) rival that of traditionally
large proteins, yet only a handful have been ascribed a function. The goal of this proposal is to propel
this nascent field forward by facilitating both small protein discovery and functional characterization. Our
preliminary data identify specific examples that clearly define cis- and trans-classes of function for short-
open-reading frames and small proteins. These early leads will be pursued to fruition, providing the
framework for the expanded rigorous study needed in any new field. We will test an additional subset of
small proteins for function, which we anticipate will reveal functions for each member of this training set,
while also establishing general principles for short-open-reading frames and small proteins.
We will develop and apply our small protein approaches in mycobacteria. Mycobacteria offer many
advantages for small protein study. Foremost is that they express >1000 small proteins in standard
conditions. An extensive toolkit for modifying, culturing, and analyzing mycobacteria makes them very
tractable. A GC-rich genome provides codon bias selection as one criterion to identify functional small
proteins. Moreover, our findings of small gene/protein function in standard laboratory conditions may
directly provide insights into the biology and pathogenesis of infection. This proposal integrates the
complementary expertise of investigators whose ongoing collaboration has already provided the requisite
groundwork leading to this proposal. Through the proposed Aims, we will identify new functional roles of
encoded mycobacterial small proteins and develop an optimized, small-proteomics pipeline for efficient
application to other bacteria, archaea, and eukaryotes.
过去的几十年见证了分子生物学领域的诞生和成熟。最初,突变体
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
KEITH M DERBYSHIRE其他文献
KEITH M DERBYSHIRE的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('KEITH M DERBYSHIRE', 18)}}的其他基金
Dissecting and connecting the SigM stimulus and ESX-4 secretory response in mycobacteria
剖析并连接分枝杆菌中的 SigM 刺激和 ESX-4 分泌反应
- 批准号:
10339992 - 财政年份:2022
- 资助金额:
$ 54.7万 - 项目类别:
Dissecting and connecting the SigM stimulus and ESX-4 secretory response in mycobacteria
剖析并连接分枝杆菌中的 SigM 刺激和 ESX-4 分泌反应
- 批准号:
10706956 - 财政年份:2022
- 资助金额:
$ 54.7万 - 项目类别:
Systematic Discovery and Analysis of Small Proteins and Small ORFs in Mycobacteria
分枝杆菌中小蛋白和小 ORF 的系统发现和分析
- 批准号:
10221007 - 财政年份:2020
- 资助金额:
$ 54.7万 - 项目类别:
Systematic Discovery and Analysis of Small Proteins and Small ORFs in Mycobacteria
分枝杆菌中小蛋白和小 ORF 的系统发现和分析
- 批准号:
10388045 - 财政年份:2020
- 资助金额:
$ 54.7万 - 项目类别:
Systematic Discovery and Analysis of Small Proteins and Small ORFs in Mycobacteria
分枝杆菌中小蛋白和小 ORF 的系统发现和分析
- 批准号:
10663206 - 财政年份:2020
- 资助金额:
$ 54.7万 - 项目类别:
Characterization of the Abundant Small Proteome of Mycobacteria
分枝杆菌丰富的小蛋白质组的表征
- 批准号:
8949153 - 财政年份:2015
- 资助金额:
$ 54.7万 - 项目类别:
Empirically Defining Gene Architecture and Expression of M. Tuberculosis
结核分枝杆菌基因结构和表达的实证定义
- 批准号:
8868643 - 财政年份:2015
- 资助金额:
$ 54.7万 - 项目类别:
Characterization of the Abundant Small Proteome of Mycobacteria
分枝杆菌丰富的小蛋白质组的表征
- 批准号:
9090002 - 财政年份:2015
- 资助金额:
$ 54.7万 - 项目类别:
Genome Scale Discovery of Mycobacterial Gene Function by Synthetic Genetic Arrays
通过合成基因阵列在基因组规模上发现分枝杆菌基因功能
- 批准号:
8567025 - 财政年份:2013
- 资助金额:
$ 54.7万 - 项目类别:
Genome Scale Discovery of Mycobacterial Gene Function by Synthetic Genetic Arrays
通过合成基因阵列在基因组规模上发现分枝杆菌基因功能
- 批准号:
8664347 - 财政年份:2013
- 资助金额:
$ 54.7万 - 项目类别:
相似海外基金
Impact of alternative polyadenylation of 3'-untranslated regions in the PI3K/AKT cascade on microRNA
PI3K/AKT 级联中 3-非翻译区的替代多聚腺苷酸化对 microRNA 的影响
- 批准号:
573541-2022 - 财政年份:2022
- 资助金额:
$ 54.7万 - 项目类别:
University Undergraduate Student Research Awards
How do untranslated regions of cannabinoid receptor type 1 mRNA determine receptor subcellular localisation and function?
1 型大麻素受体 mRNA 的非翻译区如何决定受体亚细胞定位和功能?
- 批准号:
2744317 - 财政年份:2022
- 资助金额:
$ 54.7万 - 项目类别:
Studentship
MICA:Synthetic untranslated regions for direct delivery of therapeutic mRNAs
MICA:用于直接递送治疗性 mRNA 的合成非翻译区
- 批准号:
MR/V010948/1 - 财政年份:2021
- 资助金额:
$ 54.7万 - 项目类别:
Research Grant
Translational Control by 5'-untranslated regions
5-非翻译区域的翻译控制
- 批准号:
10019570 - 财政年份:2019
- 资助金额:
$ 54.7万 - 项目类别:
Translational Control by 5'-untranslated regions
5-非翻译区域的翻译控制
- 批准号:
10223370 - 财政年份:2019
- 资助金额:
$ 54.7万 - 项目类别:
Translational Control by 5'-untranslated regions
5-非翻译区域的翻译控制
- 批准号:
10455108 - 财政年份:2019
- 资助金额:
$ 54.7万 - 项目类别:
Synergistic microRNA-binding sites, and 3' untranslated regions: a dialogue of silence
协同的 microRNA 结合位点和 3 非翻译区:沉默的对话
- 批准号:
255762 - 财政年份:2012
- 资助金额:
$ 54.7万 - 项目类别:
Operating Grants
Analysis of long untranslated regions in Nipah virus genome
尼帕病毒基因组长非翻译区分析
- 批准号:
20790351 - 财政年份:2008
- 资助金额:
$ 54.7万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Search for mRNA elements involved in the compatibility between 5' untranslated regions and coding regions in chloroplast translation
寻找参与叶绿体翻译中 5 非翻译区和编码区之间兼容性的 mRNA 元件
- 批准号:
19370021 - 财政年份:2007
- 资助金额:
$ 54.7万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Post-transcriptional Regulation of PPAR-g Expression by 5'-Untranslated Regions
5-非翻译区对 PPAR-g 表达的转录后调控
- 批准号:
7131841 - 财政年份:2006
- 资助金额:
$ 54.7万 - 项目类别: