Microflow time-resolved cytometry for FRET and fluorescent protein development
Microflow time-resolved cytometry for FRET and fluorescent protein development
批准号:
10388738
负责人:
Jessica Perea Houston
金额:
$20.0万
依托单位国家:
美国
项目类别:
财政年份:
2018
资助国家:
美国
项目状态:
已结题
起止时间:
2018-09-01 至 2023-07-31
关键词:
AcousticsApplications GrantsCell SeparationCellsCytometryDataDevelopmentEventFacultyFlow CytometryFluorescenceFluorescence Resonance Energy TransferFrequenciesFutureHispanic-serving InstitutionImageInvestigationKineticsMammalian CellMeasurementMeasuresMicrofluidicsMicroscopeMovementNew MexicoOpticsParentsProteinsRequest for ApplicationsResolutionSorting - Cell MovementStudentsSystemTimeTrainingUniversitiesValidationWorkbasecellular imagingdesigndesign and constructionfluorescence lifetime imagingfluorophoreinstrumentmicrochipmicroscopic imagingnovelscreeningtool
中文摘要
摘要
此应用程序请求为购买荧光寿命成像提供补充支持
显微镜(菲林)。这种类型的新型显微镜将大大推进该项目的目标:
R01GM129859微流时间分辨流式细胞术用于FRET和荧光蛋白开发。
薄膜系统具有对细胞成像的独特能力,从而图像是荧光的呈现
以高分辨率跨细胞测量寿命。荧光寿命是平均时间a
荧光团处于激发状态;它携带着大量关于荧光团的信息
例如微环境、近邻关系和细胞内分子的运动。这将是
支持父R01项目,因为这项工作的目标是设计和调整荧光寿命
在微芯片平台上进行细胞筛选和最终分选的流式细胞仪。《艾姆斯》将创作一部小说
微流细胞仪,当完全经过审查时,将包括许多独特的功能,如声学
通过微流控通道对细胞进行聚焦,多频率测量产生多个-
每个细胞的荧光寿命值,以及基于荧光的最终细胞分类的光电设计
终身测量。同时设计和建造了两个主要的微型流式细胞仪
该工具的应用正在计划中/正在进行中。这些是1)Förster共振能量的量化
哺乳动物细胞内的转移(FRET)事件,以及2)近红外荧光蛋白的浓缩
基于光动力学数据。因此,具有独特荧光测量能力的显微镜(FLIM)
衰变动力学之所以至关重要,有四个原因:1)它将使我们能够独立地交叉验证我们的细胞学
测量,从而提供分辨率和精确度比数量级更高的可靠性
优化;2)它将显著增强我们正在用我们的小说探索的协作应用程序
3)这将极大地扩展我们的细胞仪在其他领域的适用性,从而
扩大生物医学影响,同时支持未来的赠款申请;以及4)它将成为
新墨西哥州立大学西语裔学生和合作教师的培训和发展
服务机构。
英文摘要
Summary
This application requests supplemental support for the purchase of a fluorescence lifetime imaging
microscope (FLIM). A new microscope of this type will significantly advance the aims of the project:
R01GM129859 Microflow time-resolved Flow Cytometry for FRET and Fluorescence Protein Development.
FLIM systems have unique abilities to image cells whereby the image is a rendering of the fluorescence
lifetimes measured across cells at a high resolution. The fluorescence lifetime is the average time a
fluorophore spends in the excited state; it carries a significant amount of information about the fluorophore
such as the microenvironment, near-neighbor proximity, and movement of intracellular molecules. This will
support the parent R01 project because the objective for this work is to design and adapt fluorescence lifetime
flow cytometry onto a microchip platform for cell screening and eventual sorting. The aims will develop a novel
microflow cytometer that will, when completely vetted, incorporate many unique features such as acoustic
focusing of cells through microfluidic channels, multi-frequency measurements that give rise to multiple-
fluorescence lifetime values per cell, and electro-optical design for eventual cell sorting based on fluorescence
lifetime measurements. Concurrently with the design and construction of the microflow cytometer two major
applications of this tool are planned/underway. These are 1) the quantification of Förster resonance energy
transfer (FRET) events inside of mammalian cells, and 2) the enrichment of near-infrared fluorescent proteins
based on photo-kinetic data. Therefore a microscope (FLIM) with the unique ability to measure fluorescence
decay kinetics is essential for 4 reasons: 1) it will enable us to independently cross-validate our cytometry
measurements thereby providing resolution and precision that are orders of magnitude greater for reliable
optimization; 2) it will significantly enhance the collaborative applications that we are exploring with our novel
cytometer and current aims; 3) it will greatly expand the applicability of our cytometer for other fields thus
broadening the biomedical impact while supporting future grant applications; and 4) it will become a tool for
training and development of students and collaborating faculty at New Mexico State University, a Hispanic
Serving Institution.
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Toward the Development of an On-Chip Acoustic Focusing Fluorescence Lifetime Flow Cytometer.
致力于开发片上声聚焦荧光寿命流式细胞仪。
DOI:
10.3389/fphy.2021.647985
发表时间:
2021
期刊:
Frontiers in physics
影响因子:
3.1
作者:
[SambranoJr,Jesus, Rodriguez,Felicia, Martin,John, Houston,JessicaP]
通讯作者:
Houston,JessicaP
DOI:
10.1002/cyto.a.23606
发表时间:
2019-01
期刊:
Cytometry. Part A : the journal of the International Society for Analytical Cytology
影响因子:
--
作者:
[Alturkistany F, Nichani K, Houston KD, Houston JP]
通讯作者:
Houston JP
DOI:
10.1002/cyto.a.24207
发表时间:
2020-12
期刊:
Cytometry. Part A : the journal of the International Society for Analytical Cytology
影响因子:
--
作者:
[Nichani K, Li J, Suzuki M, Houston JP]
通讯作者:
Houston JP
DOI:
10.1002/cyto.a.24306
发表时间:
2021-03
期刊:
Cytometry. Part A : the journal of the International Society for Analytical Cytology
影响因子:
--
作者:
[Bitton A, Zheng Y, Houston JP, Houston KD]
通讯作者:
Houston KD
G-RISE at New Mexico State University
-
批准号:10558350
-
项目类别:
-
资助金额:$45.79万
-
财政年份:2023
-
负责人:Jessica Perea Houston
-
依托单位:
Microflow time-resolved cytometry for FRET and fluorescent protein development
-
批准号:10223368
-
项目类别:
-
资助金额:$28.94万
-
财政年份:2018
-
负责人:Jessica Perea Houston
-
依托单位:
Microflow time-resolved cytometry for FRET and fluorescent protein development
-
批准号:10016369
-
项目类别:
-
资助金额:$28.76万
-
财政年份:2018
-
负责人:Jessica Perea Houston
-
依托单位:
Heterogeneous excited state sorting and analysis cytometry
-
批准号:7940242
-
项目类别:
-
资助金额:$31.38万
-
财政年份:2010
-
负责人:Jessica Perea Houston
-
依托单位:
NMSU RISE to the Postdoctorate Program YRS 18-22
-
批准号:10227047
-
项目类别:
-
资助金额:$36.49万
-
财政年份:2000
-
负责人:Jessica Perea Houston
-
依托单位:
NMSU RISE to the Postdoctorate Program YRS 18-22
-
批准号:10462572
-
项目类别:
-
资助金额:$64.57万
-
财政年份:2000
-
负责人:Jessica Perea Houston
-
依托单位:
Full Project 4: Time-resolved flow cytometric study of cell signaling
-
批准号:8741944
-
项目类别:
-
资助金额:$12.59万
-
财政年份:--
-
负责人:Jessica Perea Houston
-
依托单位:
Full Project 4: Time-resolved flow cytometric study of cell signaling
-
批准号:8926864
-
项目类别:
-
资助金额:$12.08万
-
财政年份:--
-
负责人:Jessica Perea Houston
-
依托单位:
Full Project 4: Time-resolved flow cytometric study of cell signaling
-
批准号:8641899
-
项目类别:
-
资助金额:$12.11万
-
财政年份:--
-
负责人:Jessica Perea Houston
-
依托单位: