课题基金 / 基金详情

Microflow time-resolved cytometry for FRET and fluorescent protein development

Microflow time-resolved cytometry for FRET and fluorescent protein development
用于 FRET 和荧光蛋白开发的微流时间分辨细胞术
批准号:
10388738
负责人:
Jessica Perea Houston
金额:
$20.0万
依托单位国家:
美国
项目类别:
财政年份:
2018
资助国家:
美国
项目状态:
已结题
起止时间:
2018-09-01 至 2023-07-31

项目摘要

项目成果

Jessica Perea Houston的其他基金

相关文献

中文摘要
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总结
英文摘要
Summary This application requests supplemental support for the purchase of a fluorescence lifetime imaging microscope (FLIM). A new microscope of this type will significantly advance the aims of the project: R01GM129859 Microflow time-resolved Flow Cytometry for FRET and Fluorescence Protein Development. FLIM systems have unique abilities to image cells whereby the image is a rendering of the fluorescence lifetimes measured across cells at a high resolution. The fluorescence lifetime is the average time a fluorophore spends in the excited state; it carries a significant amount of information about the fluorophore such as the microenvironment, near-neighbor proximity, and movement of intracellular molecules. This will support the parent R01 project because the objective for this work is to design and adapt fluorescence lifetime flow cytometry onto a microchip platform for cell screening and eventual sorting. The aims will develop a novel microflow cytometer that will, when completely vetted, incorporate many unique features such as acoustic focusing of cells through microfluidic channels, multi-frequency measurements that give rise to multiple- fluorescence lifetime values per cell, and electro-optical design for eventual cell sorting based on fluorescence lifetime measurements. Concurrently with the design and construction of the microflow cytometer two major applications of this tool are planned/underway. These are 1) the quantification of Förster resonance energy transfer (FRET) events inside of mammalian cells, and 2) the enrichment of near-infrared fluorescent proteins based on photo-kinetic data. Therefore a microscope (FLIM) with the unique ability to measure fluorescence decay kinetics is essential for 4 reasons: 1) it will enable us to independently cross-validate our cytometry measurements thereby providing resolution and precision that are orders of magnitude greater for reliable optimization; 2) it will significantly enhance the collaborative applications that we are exploring with our novel cytometer and current aims; 3) it will greatly expand the applicability of our cytometer for other fields thus broadening the biomedical impact while supporting future grant applications; and 4) it will become a tool for training and development of students and collaborating faculty at New Mexico State University, a Hispanic Serving Institution.
期刊论文(6)
专著(0)
科研奖励(0)
会议论文
Toward the Development of an On-Chip Acoustic Focusing Fluorescence Lifetime Flow Cytometer.
致力于开发片上声聚焦荧光寿命流式细胞仪。
DOI: 10.3389/fphy.2021.647985
发表时间: 2021
期刊: Frontiers in physics
影响因子: 3.1
作者: [SambranoJr,Jesus, Rodriguez,Felicia, Martin,John, Houston,JessicaP]
通讯作者: Houston,JessicaP
DOI: 10.1002/cyto.a.23606
发表时间: 2019-01
期刊: Cytometry. Part A : the journal of the International Society for Analytical Cytology
影响因子: --
作者: [Alturkistany F, Nichani K, Houston KD, Houston JP]
通讯作者: Houston JP
DOI: 10.1002/cyto.a.24207
发表时间: 2020-12
期刊: Cytometry. Part A : the journal of the International Society for Analytical Cytology
影响因子: --
作者: [Nichani K, Li J, Suzuki M, Houston JP]
通讯作者: Houston JP
DOI: 10.1002/cyto.a.24306
发表时间: 2021-03
期刊: Cytometry. Part A : the journal of the International Society for Analytical Cytology
影响因子: --
作者: [Bitton A, Zheng Y, Houston JP, Houston KD]
通讯作者: Houston KD
G-RISE at New Mexico State University
Microflow time-resolved cytometry for FRET and fluorescent protein development
Microflow time-resolved cytometry for FRET and fluorescent protein development
Heterogeneous excited state sorting and analysis cytometry