Establishment of Active Chromatin Domains

项目摘要：MIRA的总体目标是定义域如何协调 基因激活是以果蝇为模型系统来定义的。我们已经确定了一个 在这一过程中起关键作用的基本转录因子(钳位)和本科生 安妮·黄将进行定义基因组定位和功能的关键实验 夹子基因中的截断突变。安妮将使用显微镜对突变体进行定位 用可用试剂和程序和mRNA-SEQ确定它们的多线染色体 功能。安妮将有机会学习使用新的管道进行rna-seq分析。 我的实验室最近开发的名为X vs.Y.Annie的湿凳子并不多 Covid带来的机会，并一直在网上学习计算课程，为她做准备 暑期研究。安妮将为我们理解 协调基因激活的调节，这是当前奖项的中心目标。

Project Summary: The overall goals of the MIRA are to define how domains of coordinate gene activation are defined using Drosophila as a model system. We have identified an essential transcription factor which is key to this process (CLAMP) and undergraduate student Annie Huang will perform key experiments defining the genomic localization and function of truncation mutants in the clamp gene. Annie will use microscopy to localize the mutants on polytene chromosomes using available reagents and protocols and mRNA-seq to define their function. Annie will have the opportunity and learn RNA-seq analysis using a new pipeline which my lab recently developed called X vs. Y. Annie has not had many wet bench opportunities due to Covid and has been taking computational courses online to prepare for her summer research. Annie will make an important contribution to our understanding of the coordinate regulation of gene activation which is a central goal of the current award.