Establishment of Active Chromatin Domains

项目摘要：MIRA的总体目标是定义如何协调 基因激活的定义使用果蝇作为模型系统。我们确定了一个 关键转录因子（CLAMP）和本科生 Annie Huang将进行关键实验，确定基因组定位和功能， 在钳基因的截断突变体。安妮会用显微镜来定位 多线染色体使用可用的试剂和方案和mRNA-seq来定义它们的 功能Annie将有机会使用新的管道学习RNA-seq分析 我的实验室最近开发的X对Y安妮没有多少湿板凳 她一直在网上学习计算课程，为自己的工作做准备。 暑期研究安妮将为我们理解 协调基因激活的调节，这是当前奖项的中心目标。

Project Summary: The overall goals of the MIRA are to define how domains of coordinate gene activation are defined using Drosophila as a model system. We have identified an essential transcription factor which is key to this process (CLAMP) and undergraduate student Annie Huang will perform key experiments defining the genomic localization and function of truncation mutants in the clamp gene. Annie will use microscopy to localize the mutants on polytene chromosomes using available reagents and protocols and mRNA-seq to define their function. Annie will have the opportunity and learn RNA-seq analysis using a new pipeline which my lab recently developed called X vs. Y. Annie has not had many wet bench opportunities due to Covid and has been taking computational courses online to prepare for her summer research. Annie will make an important contribution to our understanding of the coordinate regulation of gene activation which is a central goal of the current award.