ESTABLISHING SUB-NUCLEAR DOMAINS OF COORDINATE GENE REGULATION
建立协调基因调控的亚核域
基本信息
- 批准号:8360092
- 负责人:
- 金额:$ 4.35万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-05-01 至 2012-04-30
- 项目状态:已结题
- 来源:
- 关键词:AffinityAutistic DisorderBehavioral ResearchBiochemistryBiological ProcessBiomedical ResearchCellsChromosomesComplexCrohn&aposs diseaseDataDiseaseDosage Compensation (Genetics)Down SyndromeDrosophila genusFemaleFoundationsFundingGene Expression RegulationGenesGerm CellsGrantImmune systemLengthMammalsNational Center for Research ResourcesNuclearPilot ProjectsPrincipal InvestigatorProcessRegulationRelative (related person)ResearchResearch InfrastructureResourcesSiteSourceStudy modelsSystemTimeTranscriptUnited States National Institutes of HealthX Chromosomechromatin immunoprecipitationcostinnovationmale
项目摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
Coordinate gene regulation is a fundamental biological process essential to all cells from the germ line to the immune system. Regulation is disrupted in disorders as diverse as autism, Crohn's disease, and Down's syndrome. X-chromosome dosage compensation is one of the best models for studying this process because thousands of genes are co-regulated. Drosophila, like mammals, increase the transcript levels from a large number of diverse genes along the single male X-chromosome precisely two-fold relative to each female X-chromosome and all other chromosomes. The Drosophila Male Specific Lethal (MSL) complex is central to dosage compensation. It has long been proposed that MSL complex first identifies high affinity sites on the male X-chromosome and then spreads along its length. However, there is no temporal data to support this hypothesis due to limitations in obtaining sufficient material for biochemistry. Therefore, we will develop an innovative cell induction system to generate sufficient material to perform chromatin immunoprecipitation followed by NextGen sequencing (ChIPseq) at many time points. We expect that this pilot project will generate the first system to define how sub-nuclear domains are formed in real-time across species and provide a strong foundation for an NIH R01.
这个子项目是许多利用资源的研究子项目之一
由NIH/NCRR资助的中心拨款提供。子项目的主要支持
子项目的主要研究者可能是由其他来源提供的,
包括其他NIH来源。 列出的子项目总成本可能
代表子项目使用的中心基础设施的估计数量,
而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。
协调基因调控是从生殖系到免疫系统的所有细胞所必需的基本生物学过程。在自闭症、克罗恩病和唐氏综合症等多种疾病中,调节被破坏。X染色体剂量补偿是研究这一过程的最佳模型之一,因为成千上万的基因被共同调节。与哺乳动物一样,果蝇的单个雄性X染色体上的大量不同基因的转录水平沿着增加,相对于每个雌性X染色体和所有其他染色体,增加了两倍。果蝇雄性特异性致死(MSL)复合物是剂量补偿的核心。长期以来,人们提出MSL复合物首先识别男性X染色体上的高亲和力位点,然后沿其长度沿着扩散。然而,由于在获得足够的生物化学材料方面的限制,没有时间数据支持这一假设。因此,我们将开发一种创新的细胞诱导系统,以产生足够的材料来进行染色质免疫沉淀,然后在许多时间点进行NextGen测序(ChIPseq)。我们希望这个试点项目将产生第一个系统来定义亚核结构域如何在物种间实时形成,并为NIH R 01提供坚实的基础。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Erica Nicole Larschan其他文献
Erica Nicole Larschan的其他文献
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{{ truncateString('Erica Nicole Larschan', 18)}}的其他基金
Identification and characterization of chromatin regulators of coordinated synaptic gene expression
协调突触基因表达的染色质调节因子的鉴定和表征
- 批准号:
10391155 - 财政年份:2021
- 资助金额:
$ 4.35万 - 项目类别:
Establishing coordinate gene regulation during Drosophila dosage compensation
在果蝇剂量补偿过程中建立协调基因调控
- 批准号:
8158948 - 财政年份:2011
- 资助金额:
$ 4.35万 - 项目类别:
Establishing coordinate gene regulation during Drosophila dosage compensation
在果蝇剂量补偿过程中建立协调基因调控
- 批准号:
8511730 - 财政年份:2011
- 资助金额:
$ 4.35万 - 项目类别:
Establishing coordinate gene regulation during Drosophila dosage compensation
在果蝇剂量补偿过程中建立协调基因调控
- 批准号:
8710260 - 财政年份:2011
- 资助金额:
$ 4.35万 - 项目类别:
Establishing coordinate gene regulation during Drosophila dosage compensation
在果蝇剂量补偿过程中建立协调基因调控
- 批准号:
8306798 - 财政年份:2011
- 资助金额:
$ 4.35万 - 项目类别:
Establishing coordinate gene regulation during Drosophila dosage compensation
在果蝇剂量补偿过程中建立协调基因调控
- 批准号:
8738099 - 财政年份:2011
- 资助金额:
$ 4.35万 - 项目类别:
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