The Exercise and Physical Activity Collaborative Team (ExPACT): a Proposed MoTrPAC Clinical Center

体力活动分子传感器联合会(MoTrPAC)旨在发现和 描述构成物理效应的分子换能器(分子图谱)的范围 人体活动(PA)。我们组成了一个跨学科的团队，拥有必要的经验来做出贡献 对MoTrPAC的整体成功意义重大。我们的运动和身体活动协作组 (ExPACT)-阿拉巴马大学伯明翰运动医学中心(UAB)的联盟， 佛罗里达州奥兰多(TRI-MD)和鲍尔州立大学代谢与糖尿病转化研究所 大学人类行为实验室(BSU)-具有执行高度受控的丰富经验 在健康和疾病人群中进行有氧(AT)和阻力(RT)运动训练的临床试验。我们有 最大限度地利用人类生物标本(肌肉、脂肪、血液)揭示分子的漫长历史 与急性和慢性运动刺激的生理和临床结果相关的基础。此外， ExPACT的首要目标是为MoTrPAC的成功规划、执行、 和创新。第一阶段(规划年)目标1。我们将与MoTrPAC领导人和NIH有效合作 领导帮助指导和最终确定结构和标准操作程序(SOP)。目标2. 利用致力于ExPACT的机构资源和我们以前的经验，我们建议收集 试点生物样品，以促进MoTrPAC化学分析站点优化分析的能力。第二阶段 (招募和测试2-5年)目标1.我们将招收420名久坐但身体健康的男性和女性18岁 进入一项严格控制的、为期12周的随机试验，分别为AT(n=185)、RT(n=185)或不锻炼对照(CON， N=50)。ExPACT地理将丰富MoTrPAC的种族、民族、城市、郊区和农村多样性。设计 结合了全面的表型分析，以评估健康和临床结果，以及人体组织 表型分型将分子转导与运动的关键生物反应联系起来。目标2.事前和事后- 通过训练，我们将在急性运动前后获得具有战略意义的适时生物标本。为了更好地理解 在生物极端情况下，我们将在训练有素的运动员(AT(n=20))中进行同样的急性反应研究 或RT(n=20)。CON的连续生物样品收集将允许估计生物、季节、昼夜和 方法论的可变性。目标3.为了加强MoTrPAC的整体科学影响，我们将寻求更多 资助进行创新的辅助研究，这将显著增强受试者和组织的表型鉴定 以及对分子图谱的解释。第三阶段(结业第六年)目标1.我们将完成所有ExPACT 与MoTrPAC协调中心合作，进行数据录入、质量控制和中央存管程序 我们将积极为完成生物样品分析、手稿写作和 传播成果。我们将非常荣幸地与MoTrPAC合作，贡献未来关于 体力活动可以改善或保持健康的分子机制。

The Molecular Transducers of Physical Activity Consortium (MoTrPAC) is designed to discover and characterize the range of molecular transducers (the `molecular map') that underlie the effects of physical activity (PA) in humans. We formed an interdisciplinary team with the requisite experiences to contribute significantly to the overall success of MoTrPAC. Our Exercise and Physical Activity Collaborative Team (ExPACT) – an alliance of the University of Alabama at Birmingham Center for Exercise Medicine (UAB), Translational Research Institute for Metabolism and Diabetes in Orlando, Florida (TRI-MD), and Ball State University Human Performance Laboratory (BSU) – has extensive experience performing highly-controlled aerobic (AT) and resistance (RT) exercise training clinical trials in healthy and diseased populations. We have a lengthy history of maximizing human biospecimens (muscle, adipose, blood) to reveal molecular underpinnings linked to physiological and clinical outcomes of acute and chronic exercise stimuli. Moreover, the overarching goal of ExPACT is to contribute substantively to successful MoTrPAC planning, execution, and innovation. Phase I (Planning Year) Aim 1. We will effectively collaborate with MoTrPAC leaders and NIH leadership to help guide and finalize the structure and standard operating procedures (SOPs). Aim 2. Leveraging institutional resources committed to ExPACT and our previous experience, we propose to collect pilot biospecimens to facilitate the MoTrPAC Chemical Analysis Sites' ability to optimize analyses. Phase II (Recruitment and Testing Years 2-5) Aim 1. We will enroll 420 sedentary but healthy men and women 18+ y into a rigorously controlled, 12-wk, randomized trial of AT (n=185), RT (n=185), or no-exercise control (CON, n=50). ExPACT geography will enrich MoTrPAC racial, ethnic, urban, suburban, and rural diversity. The design incorporates comprehensive phenotyping to assess fitness and clinical outcomes, and human tissue phenotyping to link the molecular transducers with key biological responses to exercise. Aim 2. Pre- and post- training, we will obtain strategically timed biospecimens before and after acute exercise. To better understand the biological extremes, we will conduct the same acute response studies in highly-trained athletes (AT (n=20) or RT (n=20)). Serial biospecimen collection in CON will allow estimation of biological, seasonal, circadian and methodological variability. Aim 3. To enhance the overall scientific impact of MoTrPAC, we will seek additional funding to conduct innovative ancillary studies, which will markedly augment subject and tissue phenotyping and interpretation of the molecular maps. Phase III (Close-out Year 6) Aim 1. We will complete all ExPACT data entry, quality control, and central deposit procedures in collaboration with the MoTrPAC Coordinating Center, and we will actively contribute to the completion of biospecimen analyses, manuscript writing, and dissemination of results. We would be honored to collaborate with MoTrPAC to contribute future knowledge on the molecular mechanisms through which physical activity can improve or preserve health.