The Exercise and Physical Activity Collaborative Team (ExPACT): a Proposed MoTrPAC Clinical Center

身体活动分子传感器联盟 (MoTrPAC) 旨在发现和 表征物理效应下分子传感器的范围（“分子图”） 人类活动（PA）。我们组建了一支跨学科团队，拥有必要的经验来做出贡献 对 MoTrPAC 的整体成功具有重大意义。我们的锻炼和体育活动协作团队 (ExPACT) – 阿拉巴马大学伯明翰运动医学中心 (UAB) 的联盟， 佛罗里达州奥兰多 (TRI-MD) 和鲍尔州立大学代谢和糖尿病转化研究所 大学人类表现实验室 (BSU) – 拥有丰富的高度控制执行经验 在健康和患病人群中进行有氧 (AT) 和抗阻 (RT) 运动训练临床试验。我们有 最大化人类生物样本（肌肉、脂肪、血液）以揭示分子生物学的悠久历史 与急性和慢性运动刺激的生理和临床结果相关的基础。而且， ExPACT 的总体目标是为 MoTrPAC 的成功规划、执行、 和创新。第一阶段（规划年）目标 1. 我们将与 MoTrPAC 领导者和 NIH 有效合作 领导层帮助指导和最终确定结构和标准操作程序 (SOP)。目标2。 利用致力于 ExPACT 的机构资源和我们之前的经验，我们建议收集 试点生物样本，以促进 MoTrPAC 化学分析中心优化分析的能力。第二阶段 （招募和测试第 2-5 年） 目标 1. 我们将招募 420 名久坐但健康的 18 岁男性和女性 进入一项严格控制的、为期 12 周的随机试验 AT (n=185)、RT (n=185) 或无运动对照 (CON, n=50）。 ExPACT 地理将丰富 MoTrPAC 种族、民族、城市、郊区和农村的多样性。设计 结合全面的表型分析来评估健康和临床结果以及人体组织 表型分析将分子传感器与运动的关键生物反应联系起来。目标 2. 事前和事后 训练时，我们将在急性运动前后战略性地定时获取生物样本。为了更好地理解 生物极端情况下，我们将在训练有素的运动员中进行相同的急性反应研究（AT（n = 20） 或 RT (n=20))。 CON 中的连续生物样本收集将允许估计生物、季节、昼夜节律和 方法论的可变性。目标 3. 为了增强 MoTrPAC 的整体科学影响，我们将寻求更多 资助进行创新的辅助研究，这将显着增强受试者和组织表型分析 和分子图的解释。第三阶段（第 6 年收尾）目标 1. 我们将完成所有 ExPACT 与 MoTrPAC 协调合作进行数据输入、质量控制和中央存款程序 中心，我们将积极协助完成生物样本分析、稿件撰写和 传播结果。我们很荣幸与 MoTrPAC 合作，贡献未来的知识 身体活动可以改善或保持健康的分子机制。

The Molecular Transducers of Physical Activity Consortium (MoTrPAC) is designed to discover and characterize the range of molecular transducers (the `molecular map') that underlie the effects of physical activity (PA) in humans. We formed an interdisciplinary team with the requisite experiences to contribute significantly to the overall success of MoTrPAC. Our Exercise and Physical Activity Collaborative Team (ExPACT) – an alliance of the University of Alabama at Birmingham Center for Exercise Medicine (UAB), Translational Research Institute for Metabolism and Diabetes in Orlando, Florida (TRI-MD), and Ball State University Human Performance Laboratory (BSU) – has extensive experience performing highly-controlled aerobic (AT) and resistance (RT) exercise training clinical trials in healthy and diseased populations. We have a lengthy history of maximizing human biospecimens (muscle, adipose, blood) to reveal molecular underpinnings linked to physiological and clinical outcomes of acute and chronic exercise stimuli. Moreover, the overarching goal of ExPACT is to contribute substantively to successful MoTrPAC planning, execution, and innovation. Phase I (Planning Year) Aim 1. We will effectively collaborate with MoTrPAC leaders and NIH leadership to help guide and finalize the structure and standard operating procedures (SOPs). Aim 2. Leveraging institutional resources committed to ExPACT and our previous experience, we propose to collect pilot biospecimens to facilitate the MoTrPAC Chemical Analysis Sites' ability to optimize analyses. Phase II (Recruitment and Testing Years 2-5) Aim 1. We will enroll 420 sedentary but healthy men and women 18+ y into a rigorously controlled, 12-wk, randomized trial of AT (n=185), RT (n=185), or no-exercise control (CON, n=50). ExPACT geography will enrich MoTrPAC racial, ethnic, urban, suburban, and rural diversity. The design incorporates comprehensive phenotyping to assess fitness and clinical outcomes, and human tissue phenotyping to link the molecular transducers with key biological responses to exercise. Aim 2. Pre- and post- training, we will obtain strategically timed biospecimens before and after acute exercise. To better understand the biological extremes, we will conduct the same acute response studies in highly-trained athletes (AT (n=20) or RT (n=20)). Serial biospecimen collection in CON will allow estimation of biological, seasonal, circadian and methodological variability. Aim 3. To enhance the overall scientific impact of MoTrPAC, we will seek additional funding to conduct innovative ancillary studies, which will markedly augment subject and tissue phenotyping and interpretation of the molecular maps. Phase III (Close-out Year 6) Aim 1. We will complete all ExPACT data entry, quality control, and central deposit procedures in collaboration with the MoTrPAC Coordinating Center, and we will actively contribute to the completion of biospecimen analyses, manuscript writing, and dissemination of results. We would be honored to collaborate with MoTrPAC to contribute future knowledge on the molecular mechanisms through which physical activity can improve or preserve health.