The Exercise and Physical Activity Collaborative Team (ExPACT): a Proposed MoTrPAC Clinical Center

物理活动联盟的分子传感器（MoTrPAC）旨在发现和 表征分子转换器（“分子图谱”）的范围，这些分子转换器是物理效应的基础。 活性（PA）。我们组建了一个跨学科团队，拥有必要的经验， 这对MoTrPAC的整体成功至关重要。我们的运动和体育活动协作团队 （ExPACT）-伯明翰运动医学中心（UAB）的亚拉巴马大学联盟， 佛罗里达奥兰多代谢和糖尿病转化研究所（TRI-MD）和鲍尔州 大学人类绩效实验室（BSU）-具有丰富的经验，执行高度控制的 有氧（AT）和阻力（RT）运动训练的健康和患病人群的临床试验。我们有 最大化人类生物标本（肌肉，脂肪，血液）以揭示分子生物学的漫长历史 与急性和慢性运动刺激的生理和临床结果相关的基础。此外，委员会认为， ExPACT的总体目标是为成功的MoTrPAC规划，执行， 创新添第一阶段（规划年度）目标1.我们将与MoTrPAC领导人和NIH有效合作 领导，以帮助指导和最终确定结构和标准操作程序（SOP）。目标2. 利用致力于ExPACT的机构资源和我们以往的经验，我们建议收集 试点生物样本，以促进MoTrPAC化学分析中心优化分析的能力。二期 （招聘和测试年2-5）目标1。我们将招募420名久坐但健康的男性和女性18岁以上 纳入AT（n=185）、RT（n = 185）或无运动对照（CON， n=50）。ExPACT地理将丰富MoTrPAC种族，民族，城市，郊区和农村的多样性。设计 结合了全面的表型分析，以评估健身和临床结果，以及人体组织 表型分析将分子转换器与运动的关键生物反应联系起来。目标2.前后 训练时，我们将在急性运动前后获得战略性定时生物标本。更好地了解 生物极端，我们将在训练有素的运动员中进行相同的急性反应研究（AT（n=20）） 或RT（n=20））。CON中的连续生物标本采集将允许估计生物学、季节性、昼夜节律和 方法的可变性。目标3。为了提高MoTrPAC的整体科学影响，我们将寻求更多的 资助进行创新的辅助研究，这将显着增加受试者和组织表型 和分子图谱的解释。第三阶段（第6年结束）目标1。我们将完成所有ExPACT 与运输和公共行政部协调部门合作，执行数据输入、质量控制和中央存款程序 中心，我们将积极促进完成生物标本分析，手稿写作， 传播成果。我们很荣幸能与MoTrPAC合作，为未来的知识贡献力量。 身体活动可以改善或保持健康的分子机制。

The Molecular Transducers of Physical Activity Consortium (MoTrPAC) is designed to discover and characterize the range of molecular transducers (the `molecular map') that underlie the effects of physical activity (PA) in humans. We formed an interdisciplinary team with the requisite experiences to contribute significantly to the overall success of MoTrPAC. Our Exercise and Physical Activity Collaborative Team (ExPACT) – an alliance of the University of Alabama at Birmingham Center for Exercise Medicine (UAB), Translational Research Institute for Metabolism and Diabetes in Orlando, Florida (TRI-MD), and Ball State University Human Performance Laboratory (BSU) – has extensive experience performing highly-controlled aerobic (AT) and resistance (RT) exercise training clinical trials in healthy and diseased populations. We have a lengthy history of maximizing human biospecimens (muscle, adipose, blood) to reveal molecular underpinnings linked to physiological and clinical outcomes of acute and chronic exercise stimuli. Moreover, the overarching goal of ExPACT is to contribute substantively to successful MoTrPAC planning, execution, and innovation. Phase I (Planning Year) Aim 1. We will effectively collaborate with MoTrPAC leaders and NIH leadership to help guide and finalize the structure and standard operating procedures (SOPs). Aim 2. Leveraging institutional resources committed to ExPACT and our previous experience, we propose to collect pilot biospecimens to facilitate the MoTrPAC Chemical Analysis Sites' ability to optimize analyses. Phase II (Recruitment and Testing Years 2-5) Aim 1. We will enroll 420 sedentary but healthy men and women 18+ y into a rigorously controlled, 12-wk, randomized trial of AT (n=185), RT (n=185), or no-exercise control (CON, n=50). ExPACT geography will enrich MoTrPAC racial, ethnic, urban, suburban, and rural diversity. The design incorporates comprehensive phenotyping to assess fitness and clinical outcomes, and human tissue phenotyping to link the molecular transducers with key biological responses to exercise. Aim 2. Pre- and post- training, we will obtain strategically timed biospecimens before and after acute exercise. To better understand the biological extremes, we will conduct the same acute response studies in highly-trained athletes (AT (n=20) or RT (n=20)). Serial biospecimen collection in CON will allow estimation of biological, seasonal, circadian and methodological variability. Aim 3. To enhance the overall scientific impact of MoTrPAC, we will seek additional funding to conduct innovative ancillary studies, which will markedly augment subject and tissue phenotyping and interpretation of the molecular maps. Phase III (Close-out Year 6) Aim 1. We will complete all ExPACT data entry, quality control, and central deposit procedures in collaboration with the MoTrPAC Coordinating Center, and we will actively contribute to the completion of biospecimen analyses, manuscript writing, and dissemination of results. We would be honored to collaborate with MoTrPAC to contribute future knowledge on the molecular mechanisms through which physical activity can improve or preserve health.