Effects of standard fMRI calibrations on the diverse microvascular blood flow and oxygenation responses in cortical layers

标准功能磁共振成像校准对皮质层不同微血管血流和氧合反应的影响

• 批准号: 10394531
• 负责人: Ikbal Sencan-Egilmez
• 金额: $ 8.13万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-19 至 2022-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10394531
• 关键词: Affect; Age; Aging; Anesthesia procedures; Animal Model; Area; BRAIN initiative; Biophysics; Blood Vessels; Blood capillaries; Blood flow; Brain; Brain Pathology; Breathing; Caffeine; Caliber; Calibration; Carbon Dioxide; Catheters; Cerebrovascular Circulation; Cerebrum; Chronic; Clinical; Complex; Computer Models; Coupled; Data; Development; Environment; Faculty; Functional Magnetic Resonance Imaging; Gases; General Hospitals; Goals; Health; Human; Hypercapnia; Hyperoxia; Image; Imaging Device; Injections; Intake; Intraventricular; Ipsilateral; Link; Maps; Massachusetts; Measurement; Mentors; Metabolism; Methodology; Methods; Modeling; Morphology; Mus; Nature; Neurons; Neurosciences; Noise; Optics; Oxygen; Partial Pressure; Pathologic; Pharmacologic Substance; Positioning Attribute; Procedures; Radiology Specialty; Research; Research Design; Research Methodology; Resolution; Rest; Shoulder; Signal Transduction; Spin Labels; Technology; Time; Tissues; Training; Vibrissae; Visual; Work; aging brain; arteriole; awake; base; bioimaging; biophysical model; blood oxygen level dependent; career development; experimental study; healthy aging; hemodynamics; imaging modality; improved; insight; interdisciplinary collaboration; medical schools; metabolic rate; microscopic imaging; middle age; neuroimaging; neurovascular coupling; next generation; novel; response; tenure track; tool; training opportunity; two-photon; venule

Project Summary/Abstract: This proposed project focus on quantifying the spatial and temporal diversity in cortical oxygen metabolism and neurovascular coupling, and informing next generation of biophysical models of the Blood Oxygen Level Dependent (BOLD) functional Magnetic Resonance Imaging (fMRI) measurements by integrating new technological and conceptual approaches. Broad and long term objectives of this application are (1) to bridge understanding of brain in action at multiple scales in spatial (subcapillary to whole- brain) and temporal (µs to months) domains, (2) to contribute discovering diversity of hemodynamic responses across cortical tissue layers, functional areas, and microvascular branches, and (3) to support the modelling efforts of BOLD signal by to quantifying hemodynamic responses in cortex of healthy-aging awake mice. Realizing these objectives would contribute to the joined efforts towards improving human health through accomplishing BRAIN Initiative goals of understanding the brain in action, discovering diversity, mapping/linking activity across spatial and temporal scales, and understanding the biophysical basis of fMRI signal. The specific aims of the proposed project can be summarized as: (1) to develop faster, deeper, longitudinal measurements of the cerebral blood flow (CBF), partial pressure of oxygen (pO2), and cerebral metabolic rate of oxygen (CMRO2), (2) to quantify the diversity of CMRO2 and CBF responses to functional activation across different cortical functional areas, cortical layers, and microvasculature types in healthy-aging mouse, and (3) to quantify the effects of standard fMRI calibrations (e.g. hypercapnia, hyperoxia, and caffeine) on the CMRO2 and CBF at rest and during functional activation. The research design and methods that will be used in this project will include (1) developing faster and deeper chronic imaging of absolute oxygen concentration to assess relation between cortical pO2, CMRO2, and blood flow changes during brain activation across the cortical areas and layers, and (2) realistic measurements of the oxygenation, blood flow, and metabolism responses to functional activation at the microvascular scales in healthy-aging mice and in response to calibration procedures that are commonly used in BOLD fMRI imaging. The research environment of Dr. Sencan (the Athinoula A. Martinos Center for Biomedical Imaging at Massachusetts General Hospital and Radiology Department in Harvard Medical School) is equipped with state-of-the-art technologies required to execute the proposed project and is rich with opportunities for training in neuroscience, career development, and interdisciplinary collaborations. She has a team of mentors, collaborators and advisors with diverse and strong expertise, who are committed to support Dr. Sencan’s research, and her career development. All these factors will facilitate the successful execution of the proposed project, the completion of Dr. Sencan’s training in neuroimaging, and her transition to an independent tenure-track faculty position.

项目摘要/摘要：该提议的项目着重于量化空间和临时多样性 皮质氧代谢和神经血管耦合，并告知下一代生物物理模型 血氧水平依赖性（粗体）功能磁共振成像（fMRI）测量值 通过整合新的技术和概念方法。这是广泛而长期的目标 应用是（1）在空间中多个尺度上的大脑理解（整个毛细血管的多个尺度） 大脑）和临时（µs至数月）域，（2）有助于发现血液动力学反应的多样性 跨皮质组织层，功能区域和微血管分支，（3）支持建模 大胆信号通过量化健康清醒小鼠皮质中血液动力学反应的努力。 意识到这些目标将有助于通过 实现大脑主动性的目标，以了解行动中的大脑，发现多样性， 跨空间和临时尺度的映射/连接活动，并了解fMRI的生物物理基础 信号。拟议项目的具体目的可以总结为：（1）更快，更深，更深 大脑血流（CBF），氧气（PO2）和大脑的纵向测量 氧的代谢速率（CMRO2），（2）量化CMRO2和CBF对功能的多样性 在健康衰老中，不同皮质功能区域，皮质层和微脉管系统的激活 小鼠和（3）量化标准fMRI校准的影响（例如，高碳酸盐，高氧和咖啡含量） 在静止和功能激活期间，在CMRO2和CBF上。研究设计和方法 在该项目中使用的将包括（1）绝对氧的更快，更深的慢性成像 浓缩以评估皮质PO2，CMRO2和大脑激活过程中血流变化之间的关系 在皮质区域和层之间，以及（2）氧合，血流和 在健康小鼠和中的微血管尺度上对功能激活的代谢反应 对大胆fMRI成像中通常使用的校准程序的响应。研究环境 Sencan博士（马萨诸塞州总医院的Athinoula A. Martinos生物医学成像中心 哈佛医学院的放射科）配备了最先进的技术 执行拟议的项目，并有丰富的神经科学，职业发展培训的机会， 和跨学科合作。她有一支由多样化的导师，合作者和顾问团队 强大的专业知识，他们致力于支持Sencan博士的研究以及她的职业发展。所有这些 因素将促进拟议项目的成功执行，Sencan博士在 神经影像，她向独立的终身教师职位过渡。