Proteomics Core

蛋白质组学核心

基本信息

批准号：
10395946
负责人：
RICHARD R. DRAKE
金额：
$ 17.81万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-04-15 至 2025-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10395946
关键词：
Adopted Area Binding Binding Proteins Cells Chemicals Clinical Complex Coupled Digestive System Disorders Disease Ensure Equipment Evaluation Extracellular Matrix Extracellular Matrix Proteins Faculty Fibrosis Gastrointestinal tract structure Glycolipids Goals Histologic Image Imaging technology In Situ Infrastructure Injury Investigation Label Lipids Liquid Chromatography Liver Liver diseases Location Mass Spectrum Analysis Medical Metabolic Methodology Methods Molecular Polysaccharides Post Translational Modification Analysis Post-Translational Protein Processing Process Protein Analysis Proteins Proteomics Regulation Research Resources Running South Carolina Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization System Techniques Technology Tissues Universities base imaging approach innovation instrumentation mass spectrometric imaging member new technology novel protein expression small molecule tandem mass spectrometry translational proteomics

项目摘要

Proteomics Core - Abstract The DDRCC Proteomics Core proposes to provide DDRCC members with cutting edge proteomics to support the Centers thematic areas. It will provide equipment and technical support to run LC-MS/MS based proteomic methods for the analysis of post-translational modifications, protein interactions, and protein expression. Importantly, it will be one of a handful of facilities throughout the world that provides Mass Spec Imaging approaches to identify and characterize cellular proteins and extracellular matrix proteins in situ. The core has extended these beyond protein identification to facilitate in situ Mass Spec Imaging of metabolites, lipids, glycolipids, and glycans in normal and diseased tissues. The Core will adopt rigorous multi-partite evaluation processes to ensure that it performs optimally and provides DDRCC members access to new technologies as they are developed. Drs. Drake and Ball, who are the core's leaders, are experts in proteomic analyses. Dr. Drake has developed much of the technology that is used in Mass Spec Imaging and Dr. Ball has an extensive track record in the application of MALDI Mass Spec in the analyses of proteins and post-translational modifications. Based on our polling of the DDRCC members, we expect this core to by highly utilized.

蛋白质组学核心 - 摘要 DDRCC蛋白质组学核心建议为DDRCC成员提供最先进的蛋白质组学以支持中心主题区域。它将提供设备和技术支持以运行基于LC-MS/MS的蛋白质组学分析翻译后修饰，蛋白质相互作用和蛋白质表达的方法。重要的是，它将是全世界的少数设施之一，可提供大众规格成像鉴定和表征细胞蛋白和本地细胞外基质蛋白的方法。核心有扩展了这些超出蛋白质鉴定的超出蛋白质，以促进代谢物，脂质，脂质的原位质量成像糖脂和正常组织和患病组织中的聚糖。核心将采用严格的多目标评估流程以确保其发挥作用，并为DDRCC成员提供对新技术的访问它们是开发的。博士。 Drake和Ball是核心的领导者，是蛋白质组学分析的专家。博士德雷克（Drake 在蛋白质和翻译后分析中，MALDI质量规格应用中的记录记录修改。根据我们对DDRCC成员的民意调查，我们希望这一核心受到高度利用。