Proteomics Core

蛋白质组学核心

• 批准号: 10608991
• 负责人: RICHARD R. DRAKE
• 金额: $ 17.81万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-04-15 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10608991
• 关键词: Adopted; Area; Binding; Binding Proteins; Cells; Chemicals; Clinical; Complex; Coupled; Digestive System Disorders; Disease; Disease Progression; Equipment; Evaluation; Extracellular Matrix; Extracellular Matrix Proteins; Faculty; Fibrosis; Gastrointestinal tract structure; Glycolipids; Goals; Histologic; Image; Imaging technology; In Situ; Infrastructure; Injury; Investigation; Label; Lipids; Liquid Chromatography; Liver; Liver diseases; Location; Maps; Mass Spectrum Analysis; Medical; Metabolic; Methodology; Methods; Molecular; Polysaccharides; Post Translational Modification Analysis; Post-Translational Protein Processing; Process; Protein Analysis; Proteins; Proteomics; Regulation; Research; Resources; Running; South Carolina; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; System; Techniques; Technology; Tissues; Universities; imaging approach; innovation; instrumentation; mass spectrometric imaging; member; new technology; novel; protein expression; small molecule; tandem mass spectrometry; translational proteomics

Proteomics Core - Abstract The DDRCC Proteomics Core proposes to provide DDRCC members with cutting edge proteomics to support the Centers thematic areas. It will provide equipment and technical support to run LC-MS/MS based proteomic methods for the analysis of post-translational modifications, protein interactions, and protein expression. Importantly, it will be one of a handful of facilities throughout the world that provides Mass Spec Imaging approaches to identify and characterize cellular proteins and extracellular matrix proteins in situ. The core has extended these beyond protein identification to facilitate in situ Mass Spec Imaging of metabolites, lipids, glycolipids, and glycans in normal and diseased tissues. The Core will adopt rigorous multi-partite evaluation processes to ensure that it performs optimally and provides DDRCC members access to new technologies as they are developed. Drs. Drake and Ball, who are the core's leaders, are experts in proteomic analyses. Dr. Drake has developed much of the technology that is used in Mass Spec Imaging and Dr. Ball has an extensive track record in the application of MALDI Mass Spec in the analyses of proteins and post-translational modifications. Based on our polling of the DDRCC members, we expect this core to by highly utilized.

蛋白质组学核心-摘要 DDRCC蛋白质组学核心建议为DDRCC成员提供尖端蛋白质组学，以支持 中心的主题领域。它将提供设备和技术支持，以运行基于LC-MS/MS的蛋白质组学 翻译后修饰、蛋白质相互作用和蛋白质表达的分析方法。 重要的是，它将成为世界上少数几个提供质谱成像的设施之一 方法，以确定和表征细胞蛋白和细胞外基质蛋白在原位。核心 将这些技术扩展到蛋白质鉴定之外，以促进代谢物，脂质， 糖脂和聚糖。核心将采用严格的多方评估 流程，以确保其最佳性能，并为DDRCC成员提供新技术， 它们是发达的。德雷克博士和鲍尔博士是核心小组的领导人，他们是蛋白质组学分析方面的专家。博士 德雷克开发了大部分用于质谱成像的技术，鲍尔博士对质谱成像技术有着广泛的了解。 在蛋白质和翻译后分析中应用MALDI质谱的记录 修改.根据我们对DDRCC成员的民意调查，我们预计这一核心将得到高度利用。