Nevada Center of Biomedical Research Excellence in Molecular and Cellular Signal Transduction in the Cardiovascular System

我们的第一阶段生物研究卓越中心（COBRE）的总体目标是 建立内华达州COBRE在分子和细胞信号转导的 里诺的内华达州大学（UNR）和UNR医学院的心血管系统 医学（UNR Med）作为一个可持续的，主题性的，跨学科的基础科学研究 中心这一使命将通过开发和利用最先进的分子， 遗传、生物物理和生物医学成像技术。COBRE包括一个 行政核心（核心A）由项目主任斯科特厄利博士领导，两个研究 核心和5个项目负责人。冯玉梅厄利博士是转基因动物研究中心主任 基因分型和表型核心（核心B）和Normand Leblanc博士指导高空间 和时间分辨率成像核心（HSTRI;核心C），它提供了项目领导者 利用各种最先进的仪器， 用荧光指示剂标记的细胞和组织样品的分辨率成像。眼镜蛇 正在提交行政补充申请，以响应NOSI NOT-GM-21- 029，这将增强HSTRI核心的能力。本申请的具体目的是 购买新的Leica STELLARIS SP 5激光扫描共聚焦显微镜， 奥林巴斯FV 1000激光扫描共聚焦显微镜这个新系统的价格标签是 316286.70美元如果NIGMS提供250，000美元，其余66，286.70美元将通过以下途径提供： 机构支持。FV 1000系统是COBRE使用最多的显微镜 项目负责人和HSTRI核心服务的其他UNR调查员于年购买 2010.不幸的是，显微镜最近变得不可靠，120天中有51天是不可靠的。 仅在2021年就有42.5%的停机时间，因此已达到其使用寿命的极限。这些 重复的失败对中心的研究生产力产生了负面影响， 这将需要在未来找到一个更持久的解决办法。Leica STELLARIS SP 5系统 相对于Olympus FV 1000，大大提高了时间和空间分辨率， 这是我们中心的几位研究人员（所有5位COBRE项目负责人）的关键特征， 包括该中心更多成员的研究计划，以及其他UNR 生物医学研究者根据配置，显微镜具有20 nm的自适应聚焦控制 FV 1000缺乏的重新定位精度，可以执行FRET，FRAP和FLIP 实验，并可以很容易地升级到执行超分辨率显微镜。因此 Leica STELLARIS SP 5系统将取代并提升FV 1000的功能。

The overall objective of our Phase I Center of Biological Research Excellence (COBRE) is to establish the Nevada COBRE in Molecular and Cellular Signal Transduction in the Cardiovascular System at the University of Nevada, Reno (UNR) and the UNR School of Medicine (UNR Med) as a sustainable, thematic, interdisciplinary basic science research Center. This mission will be accomplished by developing and utilizing state-of-the-art molecular, genetic, biophysical, and biomedical imaging technologies. The COBRE comprises an Administration Core (Core A) headed by the Program Director, Dr. Scott Earley, two Research Cores, and 5 Project Leaders. Dr. Yumei Feng Earley is the Director of the Transgenic Animal Genotyping and Phenotyping Core (Core B) and Dr. Normand Leblanc directs the High Spatial and Temporal Resolution Imaging Core (HSTRI; Core C), which provides the Project Leaders with access to a wide array of state-of-the-art instruments enabling high spatial and temporal resolution imaging of cells and tissues samples labeled with fluorescent indicators. The COBRE is submitting an application for an Administrative Supplement in response to NOSI NOT-GM-21- 029 that will enhance the capabilities of the HSTRI Core. The Specific Aim of this application is to purchase a new Leica STELLARIS SP5 Laser Scanning Confocal Microscope to replace an old Olympus FV1000 Laser Scanning Confocal Microscope. The price tag for this new system is $316,286.70. If NIGMS provides $250,000, the remaining $66,286.70 will be provided through institutional support. The FV1000 system, which is the most utilized microscope by COBRE Project Leaders and other UNR investigators served by the HSTRI Core, was purchased in 2010. Unfortunately, the microscope has recently become unreliable with 51 out of 120 days of down time (42.5%) in 2021 alone and has thus reached the limit of its usable life. These repetitive failures have negatively impacted the research productivity of the Center, a situation that will require a more permanent solution in the future. The Leica STELLARIS SP5 system dramatically increases the temporal and spatial resolution relative to the Olympus FV1000, which are key features for several investigators in our Center (all 5 COBRE Project Leaders), including the research programs of more established members of the Center, and other UNR biomedical investigators. As configured, the microscope has Adaptive Focus Control with 20 nm repositioning accuracy, which the FV1000 lacks, and can perform FRET, FRAP, and FLIP experiments, and could be easily upgraded to perform super-resolution microscopy. Thus, the Leica STELLARIS SP5 system will replace and advance the capabilities of the FV1000.