Dietary Supplements and Inflammation Phase-2: ACQUISITION OF A MULTIPARAMETER FLOW CYTOMETER

摘要： 这项提议要求25万美元的资金和我们机构的剩余资金来购买一个高参数， 最先进的流式细胞仪、BD FAC、SYMPHONY A5细胞分析仪。这款流式细胞仪提供了新颖和 尖端技术旨在同时检测多达30个参数，用于多个研究 炎症性疾病，包括自身免疫、心血管和神经退行性疾病以及 肥胖、衰老和癌症是Cobre关于膳食补充剂和 南卡罗来纳大学(UofSC)的炎症(DSI)。区分多种细胞类型的能力 基于生物标志物、受体和功能特征，以及将面板整合到 一种单一的复合体，能够在尝试预防之后对疾病进行诊断和预后 用这种高分辨率和高通量的产品进行植物产品的处理将是最容易的 乐器。这台仪器将被安置在科布雷的流式细胞仪和成像核心中 南卡罗来纳大学医学院。该核心将服务于目标教员和飞行员的需求 项目教师以及他们的研究生和本科生、博士后研究员和技术人员。 现有的FC500等流式细胞仪是过时的，最多提供5种颜色的能力， 从而限制了可识别且不具有数据集成能力的细胞亚群。在……里面 此外，供应商不支持这些流式细胞仪，因为它们已过时，部件不可用。 此外，FACSymphony拥有5个激光器、30个参数和28个彩色探测器，可提供高性能 具有商用、低成本、易获得的荧光偶联抗体的检测能力，高 高速处理器，为每个探测器优化的光电倍增管，以及更新的分析软件 和视觉化。重要的是，该仪器可以升级到10个激光，50个参数，48种颜色 未来。这种台式仪器可以快速分析小体积的样品，最多可以检测到28个 颜色，克服了与荧光补偿相关的问题，并且用户友好。详情请参阅 建议，加州大学圣迭戈分校DSI科布雷的生物医学研究环境正在走向 无多参数流式细胞仪扩增。这一能力的缺乏对 参加本次科布雷的调查人员的研究计划以及招募有前途的 科学家们来到南加州大学。收购仪器并将其存放在已建立的核心设施中将使 使大批科研人员能够接触到它，并将大大提高研究质量和培训水平。 纳伦德拉·辛格博士，研究教授，流式细胞术、显微镜和成像技术的核心领导者 &gt；20年运行流式细胞仪的经验将监督该系统的运行。

Abstract: This proposal requests funds of $250K with remaining funds from our institution to purchase a high-parameter, state-of-the-art flow cytometer, BD FACSymphony A5 Cell Analyzer. This flow cytometer offers novel and cutting-edge technology aimed at simultaneous detection of up to 30 parameters for the study of many inflammatory diseases that include autoimmune, cardiovascular and neurodegenerative diseases as well as obesity, aging and cancer that are important areas of research in the COBRE on Dietary Supplements and Inflammation (DSI) at the University of South Carolina (UofSC). The ability to distinguish the multiple cell types based on biomarkers, receptors and functional characteristics as well as the ability to integrate the panels into a single composite that enables the diagnosis and prognosis of disease following attempts towards prevention and treatment with plant products will be most readily performed with this high-resolution and high-throughput instrument. This instrument will be housed in the Flow Cytometry and Imaging Core of the COBRE at the University of South Carolina, School of Medicine. This core will serve the needs of the Target Faculty and Pilot Project faculty as well as their graduate and undergraduate students, postdoctoral fellows and technical staff. The existing flow cytometers such as the FC500 are antiquated and offer a maximum of 5-color capability, thereby limiting the cell subpopulations that can be identified and have no data integration capability. In addition, these flow cytometers are not supported by the vendor as they are obsolete with parts not available. Further, FACSymphony has 5 lasers, 30 parameters and 28 color detectors offering high-performance detection capability with commercial, low cost, readily-available fluorochrome conjugated antibodies, high- speed processors, optimized photomultiplier tubes for each detector as well as updated software for analysis and visualization. Importantly, the instrument can be upgraded to 10 lasers, 50 parameters with 48 colors in the future. This bench-top instrument allows for rapid analysis of low volume of sample, can detect upto 28 colors, overcomes issues associated with fluorescence compensation and is user-friendly. As detailed in the proposal, the biomedical research environment in the COBRE on DSI at the UofSC is on a trajectory for expansion with no multi-parameter flow cytometer. The absence of this capability has adversely affected the research programs of the investigators participating in this COBRE as well as the recruitment of promising scientists to the UofSC. Acquisition of the instrument and housing it in an established core facility will allow access to it by a large number of investigators and will significantly enhance the research quality and training. Dr. Narendra Singh, a Research Professor and the Flow Cytometry, Microscopy and Imaging Core Leader has >20 years experience in running flow cytometry will oversee the operation of this system.