Dietary Supplements and Inflammation Phase-2: ACQUISITION OF A MULTIPARAMETER FLOW CYTOMETER

膳食补充剂和炎症第二阶段：购买多参数流式细胞仪

• 批准号: 10399319
• 负责人: Mitzi Nagarkatti
• 金额: $ 25万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10399319
• 关键词: Affect; Aging; Area; Autoimmune Diseases; Biological Markers; Biomedical Research; Cardiovascular Diseases; Cells; Centers of Research Excellence; Characteristics; Color; Complex; Computer software; Core Facility; Detection; Diagnosis; Disease; Disease Progression; Environment; Faculty; Financial compensation; Flow Cytometry; Fluorescence; Fluorochrome; Funding; Future; Housing; Image; Inflammation; Inflammatory; Institution; Lasers; Malignant Neoplasms; Neurodegenerative Disorders; Obesity; Performance; Phase; Phenotype; Physiological; Pilot Projects; Plants; Postdoctoral Fellow; Prevention; Prognosis; Reagent; Request for Proposals; Research; Research Personnel; Resolution; Running; Sampling; Scientist; South Carolina; Speed; System; Technology; Training; Tube; Universities; Update; Vendor; Visualization; antibody conjugate; base; cell analyzer; cell type; cost; data integration; detector; dietary supplements; equipment acquisition; experience; graduate student; instrument; instrumentation; medical schools; microscopic imaging; next generation; novel; operation; photomultiplier; professor; programs; receptor; receptor expression; recruit; undergraduate student; user-friendly

Abstract: This proposal requests funds of $250K with remaining funds from our institution to purchase a high-parameter, state-of-the-art flow cytometer, BD FACSymphony A5 Cell Analyzer. This flow cytometer offers novel and cutting-edge technology aimed at simultaneous detection of up to 30 parameters for the study of many inflammatory diseases that include autoimmune, cardiovascular and neurodegenerative diseases as well as obesity, aging and cancer that are important areas of research in the COBRE on Dietary Supplements and Inflammation (DSI) at the University of South Carolina (UofSC). The ability to distinguish the multiple cell types based on biomarkers, receptors and functional characteristics as well as the ability to integrate the panels into a single composite that enables the diagnosis and prognosis of disease following attempts towards prevention and treatment with plant products will be most readily performed with this high-resolution and high-throughput instrument. This instrument will be housed in the Flow Cytometry and Imaging Core of the COBRE at the University of South Carolina, School of Medicine. This core will serve the needs of the Target Faculty and Pilot Project faculty as well as their graduate and undergraduate students, postdoctoral fellows and technical staff. The existing flow cytometers such as the FC500 are antiquated and offer a maximum of 5-color capability, thereby limiting the cell subpopulations that can be identified and have no data integration capability. In addition, these flow cytometers are not supported by the vendor as they are obsolete with parts not available. Further, FACSymphony has 5 lasers, 30 parameters and 28 color detectors offering high-performance detection capability with commercial, low cost, readily-available fluorochrome conjugated antibodies, high- speed processors, optimized photomultiplier tubes for each detector as well as updated software for analysis and visualization. Importantly, the instrument can be upgraded to 10 lasers, 50 parameters with 48 colors in the future. This bench-top instrument allows for rapid analysis of low volume of sample, can detect upto 28 colors, overcomes issues associated with fluorescence compensation and is user-friendly. As detailed in the proposal, the biomedical research environment in the COBRE on DSI at the UofSC is on a trajectory for expansion with no multi-parameter flow cytometer. The absence of this capability has adversely affected the research programs of the investigators participating in this COBRE as well as the recruitment of promising scientists to the UofSC. Acquisition of the instrument and housing it in an established core facility will allow access to it by a large number of investigators and will significantly enhance the research quality and training. Dr. Narendra Singh, a Research Professor and the Flow Cytometry, Microscopy and Imaging Core Leader has >20 years experience in running flow cytometry will oversee the operation of this system.

摘要： 该提案要求25万美元的资金与我们机构的剩余资金用于购买高参数， 最先进的流式细胞仪，BD FACSymphony A5细胞分析仪。该流式细胞仪提供了新颖的和 尖端技术，旨在同时检测多达30个参数，用于研究许多 炎性疾病，包括自身免疫性疾病、心血管疾病和神经退行性疾病，以及 肥胖、衰老和癌症是COBRE膳食补充剂研究的重要领域， 南卡罗来纳州大学（UofSC）的炎症（DSI）。区分多种细胞类型的能力 基于生物标志物、受体和功能特征以及将面板整合到 一种单一的复合物，能够在尝试预防后诊断和预后疾病， 用这种高分辨率和高通量的方法将最容易进行植物产品的处理 仪器该仪器将位于COBRE的流式细胞术和成像核心中， 南卡罗来纳州大学医学院。这一核心将服务于目标教师和试点的需求 项目教师以及他们的研究生和本科生，博士后研究员和技术人员。 现有的流式细胞仪，如FC 500是过时的，并提供最大的5色能力， 从而限制了可被鉴定的且不具有数据整合能力的细胞亚群。在 此外，供应商不支持这些流式细胞仪，因为它们已经过时，没有零件。 此外，FACSymphony拥有5个激光器、30个参数和28个颜色检测器， 检测能力与商业，低成本，易于获得的荧光染料结合抗体，高， 高速处理器，每个检测器的优化光电倍增管以及更新的分析软件 和可视化。重要的是，该仪器可以升级到10个激光器，50个参数，48种颜色， 未来该台式仪器可快速分析少量样品，最多可检测28 颜色，克服了与荧光补偿相关的问题，并且是用户友好的。细目见 建议，生物医学研究环境中的COBRE在DSI在UofSC是一个轨道， 扩增时不使用多参数流式细胞仪。缺乏这一能力，对 参加这次COBRE的调查人员的研究计划以及招募有前途的 科学家们向UofSC。购置该仪器并将其安置在一个已建立的核心设施中， 这将使大量的调查人员能够使用该系统，并将大大提高研究质量和培训。 博士Narendra Singh，研究教授和流式细胞术，显微镜和成像核心领导人， 超过20年的流式细胞仪运行经验将监督该系统的运行。