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Supplement: Development of an Integrated 3D Human Osteo-Mucosal Model

补充：集成 3D 人体骨粘膜模型的开发

基本信息

批准号：
10403365
负责人：
Lobat Tayebi
金额：
$ 7.55万
依托单位：
MARQUETTE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-07-15 至 2023-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10403365
关键词：
3-Dimensional 3D Print Address Adhesions Adhesives Air Alveolar Amines Animal Model Animal Testing Area Basement membrane Biocompatible Materials Biologic Characteristic Biological Biological Assay Bioreactors Bone Morphogenetic Proteins Bone Tissue Cartilage Cell-Matrix Junction Cells Chemicals Clinical Collagen Communication Complex Connective Tissue Cytokeratin Data Defect Dental Materials Dental Pulp Dentin Desmosomes Development Drug Delivery Systems Drug Evaluation Employment Encapsulated Engineering Epithelial Family suidae Fibrin Tissue Adhesive Fibroblasts Formulation Future Gelatin Glues Growth Healthcare Histology Human Immunohistochemistry Implant In Situ In Vitro Incubated Jaw Laboratories Ligaments Liquid substance Metabolic Methacrylates Methods Modeling Modification Monitor Monophenol Monooxygenase Mouth Diseases Mucous Membrane Natural regeneration Nutrient Oral Oral Surgical Procedures Oral health Oral mucous membrane structure Osteoblasts Osteocalcin Outcome Penetration Physiological Printing Procedures Production Property Quinones Reaction Sampling Side Structure Surface System Techniques Tendon structure Testing Thick Time Tissue Engineering Tissue Viability Tissues Transmission Electron Microscopy Transplantation base biomaterial compatibility bone cell growth clinically relevant covalent bond decorin design functional group improved in vitro Model in vitro testing in vivo in vivo Model interfacial keratinocyte novel novel strategies oral tissue polycaprolactone reconstruction scaffold screening soft tissue three-dimensional modeling time interval tricalcium phosphate

项目摘要

SUMMARY: The integration and stability of the hard/soft tissue interface is a major challenge in regeneration and engineering of constructs composed of widely different tissue types. The current approach is employment of biocompatible natural adhesives, such as fibrin glue. However, using such adhesives, the adhesion strength is not strong enough in physiological condition and will loosen in time. Moreover, since they act as an additional layer/material between two tissues, the healthy cellular communication between tissues will be disturbed with adverse influence on interfacial tissue development. In this project, we propose a new method for adhesion of soft/hard tissues, which addresses both of the above drawbacks. We will optimize and apply our technique in oral mucosa/bone adhesion to develop an engineered osteo-mucosal complex as a key example of a construct containing widely dissimilar tissues. Our proposed approach relies on in situ incorporation of adhesive functional groups into a cell-laden soft scaffold based on a biocompatible method, which become readily adherent to a surface treated hard scaffold. More specifically, the photocurable gelatin-based matrix encapsulating cells will be enzymatically treated to have quinone functional groups which can form covalent bonds with the amine groups on the polycaprolactone bone scaffold. The interfacial adhesion will be based on Michael reaction. We claim that this new technique has three major advantages over current methods: 1) It directly integrates two compartments of soft/hard tissues without the need for employing a third material. 2) The adhesion is very strong and will not be diminished over time. 3) The formulation will lead to in depth penetration of cells and enhanced cell growth inside the scaffolds. To examine our hypotheses, the adhesion strength will be fully evaluated in vitro quantitatively and qualitatively over time after development of the osteo-mucosal complex, and the biological characteristics will be inspected in detail. A similar construct treated by fibrin glue as the adhesive agent will be produced in parallel to be used as the control sample. Moreover, the adhesion strength of our osteo-mucosal construct will be compared with the natural oral mucosa/bone interfacial tissue in a freshly slaughtered pig’s jaw. A successful outcome from the proposed adhesion method in the osteo-mucosal complex will suggest that the technique can potentially be applied (after relevant modifications) for engineering of interfacial tissues in periodontal complex, bone/cartilage, bone/ligament, bone/tendon, and dentin/pulp complexes. The applications of the developed osteo-mucosa complex include A) clinical transplantation such as alveolar reconstruction and intraoral grafting, B) production of a clinically relevant in vitro test system and an alternative to animal test models for 1) studying the interaction of biomaterials and oral tissue, and 2) oral disease screening and evaluation of drug delivery systems.

摘要：硬/软组织界面的整合和稳定性是再生的主要挑战

项目成果

期刊论文数量（41）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Recent advances and challenges in graphene-based nanocomposite scaffolds for tissue engineering application.

DOI：
10.1002/jbm.a.37417
发表时间：
2022-06
期刊：
Journal of biomedical materials research. Part A
影响因子：
0
作者：
Z. Niknam;Faezeh Hosseinzadeh;F. Shams;Leyla Fath-Bayati;Ghader Nuoroozi;Leila Mohammadi Amirabad;
通讯作者：
Z. Niknam;Faezeh Hosseinzadeh;F. Shams;Leyla Fath-Bayati;Ghader Nuoroozi;Leila Mohammadi Amirabad;

3D-printed bi-layered polymer/hydrogel construct for interfacial tissue regeneration in a canine model.

DOI：
10.1016/j.dental.2022.06.020
发表时间：
2022-08
期刊：
DENTAL MATERIALS
影响因子：
5
作者：
Jamalpour, Mohammad Reza;Yadegari, Amir;Vahdatinia, Farshid;Amirabad, Leila Mohammadi;Jamshidi, Shokoofeh;Shojaei, Setareh;Shokri, Abbas;Moeinifard, Erfan;Omidi, Meisam;Tayebi, Lobat
通讯作者：
Tayebi, Lobat

Vascularization strategies in tissue engineering approaches for soft tissue repair.