Microbiology and Metagenomics Core
微生物学和宏基因组学核心
基本信息
- 批准号:10404439
- 负责人:
- 金额:$ 39.22万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-09-01 至 2027-08-31
- 项目状态:未结题
- 来源:
- 关键词:16S ribosomal RNA sequencingAcneAdultAnaerobic BacteriaAntibiotic ResistanceAntimicrobial ResistanceAtopic DermatitisAwarenessBacteriaBehaviorBioinformaticsBiologicalCellsChildhoodClinicalClinical ResearchCollaborationsComplexCoupledDNADataData AnalysesData SetDiseaseEducationElementsEnsureEquipment and supply inventoriesEventFermentationFibroblastsFunctional disorderGenerationsGenesGenomeGenomicsGoalsGrowthHumanImmune responseLibrariesMetabolicMetagenomicsMethodologyMicrobeMicrobial BiofilmsMicrobial GeneticsMicrobiologyModelingMusNetherton syndromeOrganismPatientsPhenotypeProceduresProcessProductionProtocols documentationPsoriasisPublishingResistance profileResolutionResourcesRibosomal RNARosaceaSamplingShotgunsSiteSkinSoftware ToolsStandardizationSwabTaxonomyTechniquesTechnologyTestingToxinTrainingTraining and InfrastructureTranslational ResearchValidationWorkcost effectiveexperiencegene functiongenetic informationin vivoinsightkeratinocytelarge datasetslipid metabolismlipidomelipidomicsmeetingsmembermetabolomemetabolomicsmetagenomemicrobialmicrobial communitymicrobiomemicrobiome researchmultiple omicsnext generationnext generation sequencingpathogenquorum sensingscreeningsequencing platformskin microbiomeskin microbiotasynergismtranscriptometranscriptomics
项目摘要
PROJECT SUMMARY/ABSTRACT
Microbiology and metagenomics core
The purpose of this core is to facilitate high-throughput characterization of the acne microbiome from clinical
samples of acne patients generated from the Acne Center of Research Translation (CORT), and to advance
analysis of the function of these microbes beyond basic genomic characterization. Next-generation sequencers
have transformed microbiome research but have proven insufficient to understand the biologic relevance of
this microbial genetic information in the skin. The acne microbiome has been partially characterized but
without understanding of how products of these diverse organisms influence the pathophysiology of acne. The
Core Director, Core Associate Director, and staff of this core have extensive experience in the generation and
analysis of metagenomic data and have successfully developed a panel of functional screens to enable
analysis of how target cells respond to the metabolic products of microbe. This requires expertise in the study
of C. acnes, the predominant bacterial species in acne that is anaerobic and fastidious, thus requiring expertise
for isolation and culture. C. acnes produces a range of fermentation metabolites and other products that can
alter host immune responses and the growth of other organisms. This core will produce libraries of bacterial
organisms from the skin, perform metagenomic analysis from skin swabs, and functionally screen libraries for
their capacity to alter the immune response of fibroblasts, keratinocytes and immunocytes relevant to the skin
of acne subjects, and analyze these observations to generate hypothesis for further testing. The core will also
work closely with the transcriptomics core and lipidomic screens at UCLA to further integrate data for validation
and discovery. This ability will transform the utility of the data to members of the CORT.
项目总结/摘要
微生物学和宏基因组学核心
该核心的目的是促进临床痤疮微生物组的高通量表征,
从痤疮研究翻译中心(CORT)产生的痤疮患者的样本,并推进
这些微生物的功能分析超出了基本的基因组特征。下一代测序仪
已经改变了微生物组研究,但已被证明不足以理解
皮肤中的微生物遗传信息。痤疮微生物组已部分表征,
而不了解这些不同生物体的产物如何影响痤疮的病理生理学。的
核心总监、核心副总监及本核心的工作人员都具有丰富的代
宏基因组数据分析,并成功开发了一组功能筛选,
分析靶细胞对微生物代谢产物的反应。这需要研究方面的专门知识
梭痤疮,痤疮中的主要细菌种类是厌氧和苛养的,因此需要专业知识
进行隔离和培养C.痤疮产生一系列发酵代谢物和其他产物,
改变宿主的免疫反应和其他生物的生长。这个核心将产生细菌库,
微生物,从皮肤拭子进行宏基因组分析,并功能性筛选文库,
它们改变与皮肤相关的成纤维细胞、角质形成细胞和免疫细胞的免疫应答的能力
并分析这些观察结果以产生用于进一步测试的假设。核心还将
与加州大学洛杉矶分校的转录组学核心和脂质组学筛选密切合作,进一步整合数据进行验证
和发现这种能力将把数据的效用转化为CORT的成员。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Richard L Gallo其他文献
MEMBRANE POTENTIAL RESPONSES OF TYPE II CELLS DURING SURFACTANT SECRETION
表面活性物质分泌过程中Ⅱ型细胞的膜电位反应
- DOI:
10.1203/00006450-198404001-01787 - 发表时间:
1984-04-01 - 期刊:
- 影响因子:3.100
- 作者:
Jacob N Finkelstein;Richard L Gallo;Robert H Notter;Donald L Shapiro - 通讯作者:
Donald L Shapiro
Microencapsulated Benzoyl Peroxide for Rosacea in Context: A Review of the Current Treatment Landscape
微囊过氧化苯甲酰治疗红斑痤疮的背景:当前治疗前景的回顾
- DOI:
- 发表时间:
2024 - 期刊:
- 影响因子:11.5
- 作者:
Seemal R. Desai;Hilary E Baldwin;James Q Del Rosso;Richard L Gallo;Neal Bhatia;Julie C. Harper;J. York;L. Gold - 通讯作者:
L. Gold
Richard L Gallo的其他文献
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{{ truncateString('Richard L Gallo', 18)}}的其他基金
Acne: a disease of lipid metabolism, microbiome and the immune response
痤疮:一种脂质代谢、微生物组和免疫反应疾病
- 批准号:
10404436 - 财政年份:2022
- 资助金额:
$ 39.22万 - 项目类别:
Quorum sensing, diversity and skin inflammation
群体感应、多样性和皮肤炎症
- 批准号:
10411990 - 财政年份:2020
- 资助金额:
$ 39.22万 - 项目类别:
Quorum sensing, diversity and skin inflammation
群体感应、多样性和皮肤炎症
- 批准号:
10189516 - 财政年份:2020
- 资助金额:
$ 39.22万 - 项目类别:
Quorum sensing, diversity and skin inflammation
群体感应、多样性和皮肤炎症
- 批准号:
10421700 - 财政年份:2020
- 资助金额:
$ 39.22万 - 项目类别:
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