Microbiology and Metagenomics Core

微生物学和宏基因组学核心

• 批准号: 10404439
• 负责人: Richard L Gallo
• 金额: $ 39.22万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2027-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10404439
• 关键词: 16S ribosomal RNA sequencing; Acne; Adult; Anaerobic Bacteria; Antibiotic Resistance; Antimicrobial Resistance; Atopic Dermatitis; Awareness; Bacteria; Behavior; Bioinformatics; Biological; Cells; Childhood; Clinical; Clinical Research; Collaborations; Complex; Coupled; DNA; Data; Data Analyses; Data Set; Disease; Education; Elements; Ensure; Equipment and supply inventories; Event; Fermentation; Fibroblasts; Functional disorder; Generations; Genes; Genome; Genomics; Goals; Growth; Human; Immune response; Libraries; Metabolic; Metagenomics; Methodology; Microbe; Microbial Biofilms; Microbial Genetics; Microbiology; Modeling; Mus; Netherton syndrome; Organism; Patients; Phenotype; Procedures; Process; Production; Protocols documentation; Psoriasis; Publishing; Resistance profile; Resolution; Resources; Ribosomal RNA; Rosacea; Sampling; Shotguns; Site; Skin; Software Tools; Standardization; Swab; Taxonomy; Techniques; Technology; Testing; Toxin; Training; Training and Infrastructure; Translational Research; Validation; Work; cost effective; experience; gene function; genetic information; in vivo; insight; keratinocyte; large datasets; lipid metabolism; lipidome; lipidomics; meetings; member; metabolome; metabolomics; metagenome; microbial; microbial community; microbiome; microbiome research; multiple omics; next generation; next generation sequencing; pathogen; quorum sensing; screening; sequencing platform; skin microbiome; skin microbiota; synergism; transcriptome; transcriptomics

PROJECT SUMMARY/ABSTRACT Microbiology and metagenomics core The purpose of this core is to facilitate high-throughput characterization of the acne microbiome from clinical samples of acne patients generated from the Acne Center of Research Translation (CORT), and to advance analysis of the function of these microbes beyond basic genomic characterization. Next-generation sequencers have transformed microbiome research but have proven insufficient to understand the biologic relevance of this microbial genetic information in the skin. The acne microbiome has been partially characterized but without understanding of how products of these diverse organisms influence the pathophysiology of acne. The Core Director, Core Associate Director, and staff of this core have extensive experience in the generation and analysis of metagenomic data and have successfully developed a panel of functional screens to enable analysis of how target cells respond to the metabolic products of microbe. This requires expertise in the study of C. acnes, the predominant bacterial species in acne that is anaerobic and fastidious, thus requiring expertise for isolation and culture. C. acnes produces a range of fermentation metabolites and other products that can alter host immune responses and the growth of other organisms. This core will produce libraries of bacterial organisms from the skin, perform metagenomic analysis from skin swabs, and functionally screen libraries for their capacity to alter the immune response of fibroblasts, keratinocytes and immunocytes relevant to the skin of acne subjects, and analyze these observations to generate hypothesis for further testing. The core will also work closely with the transcriptomics core and lipidomic screens at UCLA to further integrate data for validation and discovery. This ability will transform the utility of the data to members of the CORT.

项目总结/摘要 微生物学和宏基因组学核心 该核心的目的是促进临床痤疮微生物组的高通量表征， 从痤疮研究翻译中心（CORT）产生的痤疮患者的样本，并推进 这些微生物的功能分析超出了基本的基因组特征。下一代测序仪 已经改变了微生物组研究，但已被证明不足以理解 皮肤中的微生物遗传信息。痤疮微生物组已部分表征， 而不了解这些不同生物体的产物如何影响痤疮的病理生理学。的 核心总监、核心副总监及本核心的工作人员都具有丰富的代 宏基因组数据分析，并成功开发了一组功能筛选， 分析靶细胞对微生物代谢产物的反应。这需要研究方面的专门知识 梭痤疮，痤疮中的主要细菌种类是厌氧和苛养的，因此需要专业知识 进行隔离和培养C.痤疮产生一系列发酵代谢物和其他产物， 改变宿主的免疫反应和其他生物的生长。这个核心将产生细菌库， 微生物，从皮肤拭子进行宏基因组分析，并功能性筛选文库， 它们改变与皮肤相关的成纤维细胞、角质形成细胞和免疫细胞的免疫应答的能力 并分析这些观察结果以产生用于进一步测试的假设。核心还将 与加州大学洛杉矶分校的转录组学核心和脂质组学筛选密切合作，进一步整合数据进行验证 和发现这种能力将把数据的效用转化为CORT的成员。