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Husbandry and Management Core

畜牧与管理核心

基本信息

批准号：
10405431
负责人：
CHARLOTTE HOTCHKISS
金额：
$ 139.51万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-09-30 至 2024-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10405431
关键词：
AIDS/HIV problem Animal Model Animals Antibodies Behavioral Breeding Caring Characteristics Coccidioides immitis Cytomegalovirus Development Disease Ensure Environment Epithelial Exclusion Exhibits Exposure to Female Genes Genetic Variation HIV-1 Health Health behavior Immune response Immune system Infant Infection Infrastructure Lentivirus Lentivirus Infections Macaca Macaca mulatta Macaca nemestrina Maintenance Menstrual cycle Modernization Occupational Health Organism Pathogenicity Predisposition Primate Lentiviruses Primates Production Productivity Research Research Personnel Resources Role SIV Specific qualifier value Stress Trauma Trypanosoma cruzi U-Series Cooperative Agreements United States National Institutes of Health Vagina Viral Vector Virus Washington Women&apos s Group bioinformatics tool cost effective experience experimental study germ free condition gut microbiome interest keratinization male men&apos s group microbicide microorganism natural hypothermia nonhuman primate pathogen pre-exposure prophylaxis prevent screening vaginal microbiome vector vector-borne pathogen

项目摘要

Project Summary: Maintenance of a non-human primate SPF breeding colony requires several factors to ensure the animals' physical and behavior health, breeding production, genetic diversity, and exclusion of specified pathogens. The pigtailed macaque, M. nemestrina, exhibits additional physical and behavioral characteristics that pose additional challenges in colony maintenance. M. nemestrina exhibit greater susceptibility to the pathogenic effects of many microorganisms. Their susceptibility to lentiviruses makes them valuable animal models for HIV/AIDS research.1-11 However, nonhuman primates in standard outdoor breeding colonies are exposed to environmental and vector-borne pathogens, such as Trypanosoma cruzi and Coccidioides immitis, and M. nemestrina demonstrate enhanced susceptibility to disease with such exposure. Care must also be taken to prevent M. nemestrina infants from hypothermia. In contrast to rhesus macaques (M. mulatta) which exhibit seasonal breeding, infant M. nemestrina are born year-round and must be maintained in a heated environment. Breeding management is also different between M. mulatta and M. nemestrina. M. mulatta can be bred in large multi-male, multi-female groups in large corrals with minimal infrastructure. In contrast, M. nemestrina form single male multi-female fission/fusion groups in the wild12,13 and attempts to form multi-male groups result in significant trauma. Even with stable, single-male breeding groups, productivity is lower for M. nemestrina than for M. mulatta.14 The Washington National Primate Research Center (WaNPRC) has over 50 years of breeding experience with this species,15 and has the expertise and resources to meet these challenges. With support from this U42 cooperative agreement, we will ensure the health of the SPF M. nemestrina colony, including maintaining the animals free of B virus, SIV, SRV, and STLV, along with control of environmental organisms, such as T. cruzi and C. immitis. Using appropriate bioinformatics tools, we will increase production of SPF M. nemestrina to meet the needs of HIV/AIDS investigators in a cost-effective manner. We will provide SPF M. nemestrina to investigators in accordance with NIH-directed priorities. We will expand pre-assignment screening of animals for T. cruzi, flaviruses, CMV, and AAV.

项目总结: