MHC Genetic Typing Core

项目摘要/摘要： 猕猴是研究和治疗艾滋病及其相关疾病的重要动物模型 疾病。然而，根结线虫的基因组特征仍然有限。因此，我们的一个目标是 拟议的工作是扩大基因组分析，使用于通知基因的工具得以更新 S艾滋病研究中对新月形吸虫的管理和分配。为了实现这一目标，我们使用了 单核苷酸多态(SNPs)基因分型(GBS)与流行的次要基因 WaNPRC繁育群体中受试者之间的等位基因。这些SNP将用于支持 96个用于亲子鉴定的SNP面板。然后将对96个SNP阵列进行基因分型 96个新城疫霉菌DNA，包括6个重复样本以测试基因重复性，以及90个DNA 来自已知亲子关系的动物的样本，以测试准确的亲子鉴定。一旦验证是 完成后，SNP阵列将用于对繁殖群体进行重新基因分型，从而实现从 STR平台将SNP分型用于亲子鉴定。此外，该项目还将扩大MHC I级 WaNPRC繁育群体的等位基因单倍型分析，以确保所有SPF繁育群体的动物 具有基因特征的。

Project Summary/Abstract: Macaca nemestrina serves as a critical animal model for the study and treatment of AIDS and AIDS-related diseases. However the genomic characterization of M. nemestrina remains limited. Thus one goal of the proposed work is to expand genome analysis, enabling the update of tools used to inform the genetic management and assignment of M. nemestrina for S/HIV studies. Towards this goal, we have used genotyping-by-sequencing (GBS) to identify single nucleotide polymorphisms (SNPs) with prevalent minor alleles among subjects in the WaNPRC breeding colony. These SNPs will be used to support the design of a 96 SNP panel for use in parentage validation. The 96 SNP array will then be evaluated by genotyping a set of 96 M. nemestrina DNAs, including 6 replicate samples to test for genotype reproducibility, and 90 DNA samples from animals with known parentage to test for accurate parentage discrimination. Once validation is complete, the SNP array will be used to re-genotype the breeding colony, enabling a smooth transition from the STR platform to SNP typing for parentage assessment. In addition, this project will expand MHC Class I allele haplotype analysis of the WaNPRC breeding colony to ensure that all SPF breeding colony animals are genetically characterized.