Identification and Characterization of Endogenous Dental Pulp Stem Cells

标题 内源性牙髓干细胞的鉴定与鉴定 摘要 本研究的目的是鉴定和鉴定成人内源性牙髓干细胞。 老鼠的臼齿。尽管不断生长的老鼠门牙已经被用作模型系统 对于牙齿干细胞的特征，磨牙内的内源性DPSCs仍未被研究。与门牙不同， 老鼠的磨牙不会持续生长，与人类的牙齿相似。因此，这是至关重要的领域 干细胞生物学和牙髓再生疗法鉴定和表征内源性牙髓干细胞 从臼齿上分离出来的。为了进行这项研究，我们开发了一种小鼠模型，在该模型中，Axin2+牙科 磨牙中的牙髓细胞是基因标记的。我们的初步评估显示Axin2+DPSCs是 能够在体内自我更新和分化为成熟的成牙本质细胞一年。此外， 移植的牙髓细胞能够再生牙髓样组织。这一初步发现显示了不同之处 从其他成体干细胞和DPSCs再生的组织类型中，表明它们独特的细胞 内源性DPSCs存在可塑性。在此应用程序中，我们将测试Axin2表达的DPSCS是否能够满足 成体干细胞的标准。我们还将在单细胞水平上检查干细胞的属性。这个 将仔细评估移植的内源性DPSCs的再生能力和细胞可塑性。 Axin2+DPSCs的潜在异质性将通过单细胞RNA序列分析和 生物信息学分析。这项建议的完成具有极大的潜力来推进我们的知识 磨牙内源性DPSCs的基础，导致组织再生治疗的新策略。

Title Identification and characterization of endogenous dental pulp stem cells Abstract The goal of this proposal is to identify and characterize endogenous dental pulp stem cells (DPSCs) in adult mice molars. Although continuously growing mouse incisors have been used as a model system to characterize dental stem cells, endogenous DPSCs within the molars remain understudied. Unlike incisors, mouse molars are not continuously growing and resemble human teeth. Therefore, it is crucial for the field of stem cell biology as well as dental pulp regeneration therapy to identify and characterize endogenous DPSCs isolated from the molars. To conduct this research we have developed a mouse model in which Axin2+ dental pulp cells in molars are genetically labeled. Our preliminary assessment shows that Axin2+ DPSCs are capable of self-renewing and differentiating into mature odontoblasts for a year in vivo. In addition, the transplanted dental pulp cells are capable regenerate pulp-like tissue. This initial discovery shows a difference in the type of tissues regenerated from other adult stem cells and DPSCs, suggesting their unique cellular plasticity present in endogenous DPSCs. In this application, we will test if Axin2-expressing DPSCs can meet the criteria of adult stem cells. We will also examine the stem cells properties at a single cell level. The regenerative ability and cellular plasticity of the transplanted endogenous DPSCs will be carefully assessed. Potential heterogeneity of Axin2+ DPSCs will be identified through single-cell RNA-sequence analysis and bioinformatics analysis. The completion of this proposal has outstanding potential to advance our knowledge base of endogenous DPSCs in molars, leading to novel strategies for tissue regeneration therapies.