Acquisition of an automated crystal imager
购置自动晶体成像仪
基本信息
- 批准号:10410861
- 负责人:
- 金额:$ 17万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-04-15 至 2023-04-14
- 项目状态:已结题
- 来源:
- 关键词:Advisory CommitteesBiologicalBiophysicsCommunicable DiseasesComplexComputer softwareCrystallizationCrystallographyDNADataDiabetes MellitusDiseaseDoctor of PhilosophyEnvironmentEquipmentFacultyFundingImageImmuneInterventionLengthMaintenanceMalignant NeoplasmsManualsMolecularPharmaceutical PreparationsPoliciesProteinsResearch PersonnelResolutionRoentgen RaysScienceSeaSodium ChlorideSpeedStructureSystemTechniquesTimeTrainingUnited States National Institutes of HealthVirusWorkarmcostcrystallinityexperimental studygulf coasthigh throughput screeningimagerimaging systeminstrumentmacromoleculememberneuropsychiatric disorderprotein complexstructural biology
项目摘要
ABSTRACT
Funds are requested to acquire a JanSci UVEX-PS600 imaging system to visualize, interpret, evaluate and
record crystallization experiments generated during high throughput screening to identify crystallization
conditions for our biological macromolecules. UTMB has seven dedicated protein crystallography groups, as
well as a User Facility that serves researchers using protein crystallography as a secondary technique. Our
researchers leverage structural biology to investigate targets involved in neuropsychiatric disorders,
infectious diseases, immune defense, cancer, and diabetes-related disorders, as well as basic biological
mechanisms. The targets span a wide range of proteins, protein:protein complexes, protein:DNA complexes,
protein:drug complexes, and viruses. Many of our targets are very challenging: for example, large multi-domain
macromolecules, ternary protein complexes, glycosylated proteins and specific protein:drug complexes so that
we often need to screen thousands of crystallization conditions before promising hits are found which can be
leveraged to grow crystals suitable for high resolution diffraction experiments. To enable structure
determination, as well as speed up and decrease the cost of crystallizing our targets, we need an imaging
system that can efficiently identify successful crystallization conditions. The system must 1) drive the
science forward by identifying crystalline vs amorphous protein precipitate, and protein vs salt crystals; 2) image
and evaluate thousands of different crystallization experiments with high resolution and high speed; and 3)
handle a very large amount of crystallization data from many different projects in a multi-user environment.
Currently, the structural biology groups at UTMB are generating ~1300+ crystallization plates per year
(and rising) supported by $15M in NIH funding (9 R01s). We have no access to a working crystallization
plate imager at UTMB. Acquiring a UVEX-PS600 system and CrystalDetect/WebView software from Jan
Scientific will enable us to image crystallization experiments efficiently and at high resolution using both visible
and UV wavelengths to identity promising crystals, and provide enough imaging capacity to work with 628
crystallization plates at a time without manual intervention. The new instrument will be housed in the recently
renovated Sealy Center for Structural Biology and Molecular Biophysics (SCSB) at UTMB, and will be supported
by the SCSB. A Ph.D.-level X-ray Facility Manager, fully supported by the SCSB, will oversee the system. A
steering committee composed of a mix of users, as well as arm’s length experts, will provide oversight of the
policies relating to access, training, and maintenance, and it will be overseen by an established advisory
committee. The UVEX-PS600 system will serve our investigators carrying out NIH-funded projects at UTMB that
involve structural biology; access will also be provided to users at the SCSB and new faculty to facilitate their
competition for NIH support. In addition, members of the Gulf Coast Consortium will have access to this
equipment through the SCSB to perform pilot experiments on a wide range of structural biology projects.
摘要
需要资金以购买Jansci Uvex-PS600成像系统,以进行可视化、解释、评估和
记录高通量筛选过程中生成的结晶实验,以确定结晶
我们的生物大分子的条件。UTMB有七个专门的蛋白质结晶学小组,
以及为使用蛋白质结晶学作为二次技术的研究人员提供服务的用户设施。我们的
研究人员利用结构生物学来调查涉及神经精神障碍的目标,
传染病、免疫防御、癌症和糖尿病相关疾病以及基本生物学
机制。靶标跨越广泛的蛋白质,蛋白质:蛋白质复合体,蛋白质:DNA复合体,
蛋白质:药物复合体和病毒。我们的许多目标都非常具有挑战性:例如,大型多域
大分子、三元蛋白质复合体、糖基化蛋白质和特定蛋白质:药物复合体
我们通常需要筛选数千种结晶条件,然后才能找到有希望的匹配条件,这可能是
用来生长适合高分辨率衍射实验的晶体。启用结构
决心,以及加快和降低我们的目标结晶的成本,我们需要一个成像
该系统可以有效地识别成功的结晶条件。系统必须1)驱动
通过识别结晶型和非晶型蛋白质沉淀以及蛋白质和盐晶体来推进科学;2)图像
并对数千种不同的结晶实验进行高分辨率和高速度的评估;以及3)
在多用户环境中处理来自许多不同项目的大量结晶数据。
目前,UTMB的结构生物学小组每年生产约1300多块结晶板
(而且还在上升)得到了NIH 1500万美元资金的支持(9个R01)。我们无法接触到有效的结晶
UTMB的平板成像仪。从1月购买Uvex-PS600系统和CrystalDetect/WebView软件
科学将使我们能够高效和高分辨率地成像结晶实验,使用可见光
和UV波长来识别有希望的晶体,并提供足够的成像能力来处理628
结晶板一次完成,无需人工干预。这台新仪器将被安置在最近的
UTMB结构生物学和分子生物物理(SCSB)翻新的Sealy中心,并将得到支持
被南加州州立大学的。一名博士级别的X射线设备经理将监督该系统,并得到SCSB的全面支持。一个
由多名用户和保持距离的专家组成的指导委员会将监督
与访问、培训和维护相关的政策,并将由一个既定的咨询机构进行监督
委员会审议阶段。Uvex-PS600系统将为我们在UTMB执行NIH资助的项目的调查人员提供服务
涉及结构生物学;还将向SCSB的用户和新教员提供访问权限,以方便他们的
NIH支持的竞争。此外,墨西哥湾沿岸联盟的成员将可以访问
通过SCSB的设备,在广泛的结构生物学项目上进行中试实验。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Gabrielle Rudenko其他文献
Gabrielle Rudenko的其他文献
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{{ truncateString('Gabrielle Rudenko', 18)}}的其他基金
Molecular mechanisms of the synaptic organizer alpha-neurexin
突触组织者α-neurexin的分子机制
- 批准号:
8813887 - 财政年份:2014
- 资助金额:
$ 17万 - 项目类别:
Molecular mechanisms of the synaptic organizer alpha-neurexin
突触组织者α-neurexin的分子机制
- 批准号:
8339687 - 财政年份:2006
- 资助金额:
$ 17万 - 项目类别:
Synaptic Organizers: Dynamic Regulation of Trans-synaptic Bridges
突触组织者:跨突触桥的动态调节
- 批准号:
9910228 - 财政年份:2006
- 资助金额:
$ 17万 - 项目类别:
Molecular mechanisms of the synaptic organizer alpha-neurexin
突触组织者α-neurexin的分子机制
- 批准号:
8700516 - 财政年份:2006
- 资助金额:
$ 17万 - 项目类别:
Synaptic Organizers: Dynamic Regulation of Trans-synaptic Bridges
突触组织者:跨突触桥的动态调节
- 批准号:
10397995 - 财政年份:2006
- 资助金额:
$ 17万 - 项目类别:
Molecular mechanisms of the synaptic organizer alpha-neurexin
突触组织者α-neurexin的分子机制
- 批准号:
8496875 - 财政年份:2006
- 资助金额:
$ 17万 - 项目类别:
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