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Novel insights into nutrient-dependent regulation of beta cell proliferation

对β细胞增殖的营养依赖性调节的新见解

基本信息

批准号：
10410429
负责人：
Maike Sander
金额：
$ 43.07万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-01 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10410429
关键词：
5&apos -AMP-activated protein kinase Affect Apoptotic B Cell Proliferation B-Cell Development Beta Cell Cell Death Cell Proliferation Cell Survival Cell physiology Cells Cessation of life Couples Cues Cyclic AMP-Dependent Protein Kinases Deacetylase Development Diabetes Mellitus Energy Intake Exhibits Exposure to Failure Functional disorder Funding Genetic Models Genetic Transcription Glucose Goals Grant Homeostasis Human Hyperglycemia In Vitro Insulin Insulin Resistance Insulin-Dependent Diabetes Mellitus Laboratories Life Link MAP Kinase Gene Mediating Metabolism Molecular Mus Non-Insulin-Dependent Diabetes Mellitus Nutrient Nutritional Pancreas Pathway interactions Pharmacology Physiological Protein Kinase Proteins Proteomics Regulation Reporting Rodent Signal Pathway Signal Transduction Sirtuins Testing Therapeutic Translating Transplantation Work biological adaptation to stress cell regeneration combinatorial endoplasmic reticulum stress experimental study genetic approach genetic inhibitor glucose metabolism glycemic control in vivo inhibitor innovation insight interest islet mouse genetics new therapeutic target novel postnatal preservation prevent response small molecule stem cells synergism transcriptomics translational potential

项目摘要

A decline in functional β-cell mass and subsequent inability to maintain adequate glycemic control are hallmarks of both type 1 and type 2 diabetes. Innovative therapeutic approaches are aimed at preserving and restoring functional β-cell mass in diabetes; however, strategies to safely expand β-cell mass remain to be identified. The predominant mechanism for adapting β-cell mass to states of increased insulin demand is through modulation of β-cell replication. Therefore, there has been considerable interest in understanding the mechanisms that regulate β-cell replication with the goal of discovering new therapeutic targets to promote β-cell regeneration. Preliminary unpublished evidence from our laboratory suggests that the NAD+-dependent cytoplasmic deacetylase Sirtuin 2 (SIRT2) acts as a nutrient-dependent regulator of mitogenic signaling in rodent and human β-cells. Using mouse genetic and inhibitor approaches in human islets, we found that loss of SIRT2 activity stimulates β-cell proliferation and β-cell mass expansion under hyperglycemic conditions. We have also obtained evidence that mimicking nutrient state changes by manipulating NAD+ availability regulates β-cell proliferation in a manner consistent with SIRT2-dependent responses. Since intracellular NAD+ levels fluctuate with glucose availability, we hypothesize that SIRT2 couples β-cell proliferation to glucose metabolism. Furthermore, we have found that SIRT2 inhibits β-cell proliferation by dampening MAPK signaling and that SIRT2 inhibition in systemic hyperglycemia promotes β-cell proliferation, while protecting β-cells from activating pro-apoptotic signaling downstream of the endoplasmic reticulum (ER) stress response. In this proposal, we will explore how SIRT2 regulates mitogenic signaling as well as ER stress responses in β-cells. To accomplish this, we will pursue three Aims. In Aim 1 we will employ mouse genetic approaches and experiments in human islets to determine how glucose and nutrient state affect SIRT2-dependent regulation of β-cell proliferation. Here, we will investigate links between NAD metabolism, activity of the master regulator of cellular energy homeostasis AMPK, SIRT2 activity, and β-cell proliferation to gain mechanistic insight into the signaling cascades that couple nutrient availability to proliferation in β-cells. To understand how SIRT2 modulates intracellular signaling to affect glucose-induced proliferative and apoptotic responses in β-cells, in Aim 2, we will identify the downstream effectors of SIRT2 in the regulation of β-cell proliferation, employing proteomic as well as in vitro and in vivo approaches. Finally, in Aim 3, we will examine the effects of SIRT2 inhibition on human β-cell proliferation and function in vivo and explore whether combinatorial targeting of different mitogenic signaling pathways can augment pro-proliferative effects of SIRT2 inhibition. Together, experiments under this proposal will uncover how β-cells translate nutrient cues into mitogenic signals as well as pave the way for developing pharmacological strategies to safely increase β-cell mass in humans with diabetes.

功能性β细胞质量的下降和随后无法维持足够的血糖控制是1型和2型糖尿病的标志。创新的治疗方法旨在保存和恢复糖尿病患者的功能性β细胞群；然而，安全扩大β细胞团的策略仍有待确定。使β细胞质量适应胰岛素需求增加状态的主要机制是通过调节β细胞复制。因此，人们对了解调节β细胞复制的机制非常感兴趣，目的是发现新的治疗靶点来促进β细胞再生。我们实验室的初步未发表的证据表明，NAD+依赖性细胞质去乙酰化酶Sirtuin 2 （SIRT2）在啮齿动物和人类β细胞中作为有丝分裂信号的营养依赖性调节因子。在人类胰岛中使用小鼠遗传和抑制剂方法，我们发现SIRT2活性的丧失刺激了高血糖条件下β细胞增殖和β细胞团扩增。我们还获得了通过操纵NAD+可用性来模拟营养状态变化的证据，以与sirt2依赖性反应一致的方式调节β-细胞增殖。由于细胞内NAD+水平随葡萄糖可用性而波动，我们假设SIRT2将β细胞增殖与葡萄糖代谢结合在一起。此外，我们发现SIRT2通过抑制MAPK信号传导抑制β细胞增殖，SIRT2抑制全身性高血糖促进β细胞增殖，同时保护β细胞免于激活内质网（ER）应激反应下游的促凋亡信号传导。在本研究中，我们将探讨SIRT2如何调节β细胞中的有丝分裂信号传导和内质网应激反应。为此，我们将努力实现三个目标。在Aim 1中，我们将采用小鼠遗传方法和人类胰岛实验来确定葡萄糖和营养状态如何影响sirt2依赖的β细胞增殖调节。在这里，我们将研究NAD代谢、细胞能量稳态的主要调节因子AMPK的活性、SIRT2活性和β细胞增殖之间的联系，以获得β细胞中营养可用性与增殖耦合的信号级联的机制。为了了解SIRT2如何调节细胞内信号传导以影响葡萄糖诱导的β细胞增殖和凋亡反应，在Aim 2中，我们将采用蛋白质组学以及体外和体内方法，确定SIRT2在调节β细胞增殖中的下游效应物。最后，在Aim 3中，我们将研究SIRT2抑制对体内人β细胞增殖和功能的影响，并探索不同有丝分裂信号通路的组合靶向是否可以增强SIRT2抑制的促增殖作用。总之，在这个提议下的实验将揭示β细胞如何将营养线索转化为有丝分裂信号，并为开发安全增加糖尿病患者β细胞质量的药理学策略铺平道路。

项目成果

期刊论文数量（28）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Transcriptional mechanisms of pancreatic β-cell maturation and functional adaptation.

胰腺β细胞成熟和功能适应的转录机制。

DOI：
10.1016/j.tem.2021.04.011
发表时间：
2021-07
期刊：
Trends in endocrinology and metabolism: TEM
影响因子：
0
作者：
Wortham M;Sander M
通讯作者：
Sander M

Pancreatic Exocrine Tissue Architecture and Integrity are Maintained by E-cadherin During Postnatal Development.

在产后发育过程中，胰腺外分泌组织的结构和完整性由 E-钙粘蛋白维持。

DOI：
10.1038/s41598-018-31603-2
发表时间：
2018
期刊：
Scientific reports
影响因子：
4.6
作者：
Serrill,JeffreyD;Sander,Maike;Shih,HungPing
通讯作者：
Shih,HungPing

Embryonic ductal plate cells give rise to cholangiocytes, periportal hepatocytes, and adult liver progenitor cells.

DOI：
10.1053/j.gastro.2011.06.049
发表时间：
2011-10
期刊：
Gastroenterology
影响因子：
29.4
作者：
Carpentier R;Suñer RE;van Hul N;Kopp JL;Beaudry JB;Cordi S;Antoniou A;Raynaud P;Lepreux S;Jacquemin P;Leclercq IA;Sander M;Lemaigre FP
通讯作者：
Lemaigre FP

Loss of Pten and Activation of Kras Synergistically Induce Formation of Intraductal Papillary Mucinous Neoplasia From Pancreatic Ductal Cells in Mice.

DOI：
10.1053/j.gastro.2017.12.007
发表时间：
2018-04
期刊：
Gastroenterology
影响因子：
29.4
作者：
Kopp JL;Dubois CL;Schaeffer DF;Samani A;Taghizadeh F;Cowan RW;Rhim AD;Stiles BL;Valasek M;Sander M
通讯作者：
Sander M

Pancreas development ex vivo: culturing embryonic pancreas explants on permeable culture inserts, with fibronectin-coated glass microwells, or embedded in three-dimensional Matrigel™.