ROLE OF SOX9 IN CONTROLLING PANCREATIC PROGENITOR CELL PROPERTIES

SOX9 在控制胰腺祖细胞特性中的作用

• 批准号: 8169654
• 负责人: Maike Sander
• 金额: $ 1.19万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169654
• 关键词: Affect; Beta Cell; Cells; Characteristics; Communication; Computer Retrieval of Information on Scientific Projects Database; Embryo; Ensure; Funding; Gene Silencing; Genes; Grant; Institution; Mus; Pancreas; Property; Research; Research Personnel; Resources; Role; Source; Stem cells; Testing; United States National Institutes of Health; progenitor; stem; time use

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our lab has recently identified Sox9 as a factor that exclusively marks the stem/progenitor cell compartment in the embryonic pancreas. In this proposal, I will test how Sox9 alters the properties of a cell to ensure that the cell maintains progenitor cell characteristics. By comparing normal progenitors to Sox9-deficient progenitors, I have already determined which genes are activated by Sox9 in the cell. I found that many of these genes are important for allowing cells to make specific connections with other cells, thus ensuring communication between cells. Using time-specific gene inactivation in mice, I here propose to test how inactivation of Sox9 in multipotential pancreas progenitors affects the ability of progenitors to give rise to beta-cells. I will then test whether Sox9 controls beta-cell formation by allowing cells to make appropriate contacts with their neighbors.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们的实验室最近发现Sox 9是一种专门标记胚胎胰腺干/祖细胞区室的因子。在这个提案中，我将测试Sox 9如何改变细胞的特性，以确保细胞保持祖细胞的特征。 通过比较正常的祖细胞和Sox 9缺陷的祖细胞，我已经确定了细胞中哪些基因被Sox 9激活。 我发现这些基因中的许多基因对于允许细胞与其他细胞建立特定的连接非常重要，从而确保细胞之间的通信。 在小鼠中使用时间特异性基因失活，我在这里建议测试如何在多能胰腺祖细胞中Sox 9的失活影响祖细胞产生β细胞的能力。 然后，我将测试Sox 9是否通过允许细胞与它们的邻居进行适当的接触来控制β细胞的形成。