Core 2

核心2

• 批准号: 10411670
• 负责人: Kavita Madhav Dhodapkar
• 金额: $ 42.64万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10411670
• 关键词: Anatomy; Archives; Bioinformatics; Biological; Biological Assay; Biopsy; Biostatistics Core; Blood; Cells; Cellular Indexing of Transcriptomes and Epitopes by Sequencing; Clinical; Clinical Trials; Clinical trial protocol document; Communication; Consent; Correlative Study; Coupled; Culture Media; Cytometry; Data; Database Management Systems; Databases; Diagnosis; Ensure; Ethics; Evaluation; Fluorescence; Freezing; Gene Expression; Genomics; Goals; Histologic; Histology; Histopathology; IACUC; Image; Immune; Immune response; Immune system; Immunohistochemistry; Immunologic Monitoring; Immunologic Tests; Immunologics; Immunologist; Immunotherapy; Infrastructure; Institutional Review Boards; Investigation; Liquid substance; Lung; Lung Adenocarcinoma; Maintenance; Malignant Neoplasms; Malignant neoplasm of lung; Molecular Immunology; Monitor; Mutation; Nursing Research; Pathologic; Pathologist; Pathology; Patients; Phenotype; Procedures; Process; Proteomics; Protocols documentation; Quality Control; Research; Research Personnel; Resistance; Resource Sharing; Resources; STK11 gene; Saliva; Sampling; Scientist; Secure; Services; Slide; Specific qualifier value; Specimen; Specimen Handling; Stains; Standardization; Structure; System; Technical Expertise; Tissue Banks; Tissue Sample; Tissues; Translations; Tumor Markers; Tumor-infiltrating immune cells; Update; Work; base; central database; clinical practice; data integration; data sharing; digital; experience; genomic data; human tissue; molecular pathology; mouse model; neoplastic; preclinical study; prospective; repository; single-cell RNA sequencing; tissue archive; tool; tumor

SUMMARY The goal of the Molecular Pathology and Immunology Core (Core 2) is to provide a centralized technical infrastructure, inclusive of materials and the technical expertise necessary to accomplish all tissue-based investigations including the collection of tissue samples, histopathological analysis, immune monitoring and maintenance of a centralized database. Core 2 will function as the main repository of patient specimens and will oversee specimen processing and histopathology. Collected tissue samples will be analyzed for P01 project specified tumor biomarkers and phenotyping of tumor immune infiltrates utilizing immunohistochemistry for tumor biomarkers and mass cytometry and single cell RNA sequencing for immune cell phenotyping. Two experienced lung pathologists and an expert immunologist will work closely with the Bioinformatics and Biostatistics Core 3 and the Administrative Core 1 to ensure coordinated procurement, archiving, and storage of human tissue samples (especially clinical trial specimens) and seamless integration of the data generated from tissue-based investigations, including from mouse models and clinical trials, into a secure, database for utilization and further analysis by Cores 1 and 3 and P01 projects. All tissue-based research will be performed with ethical research oversight from IRB and IACUC protocols. Continuous communication between the clinicians, scientists, research nurses, biostatisticians, bioinformaticians and pathologists together with established standardized operating procedures for all core activities will provide optimal tissue collection, processing, analysis, and data sharing for all projects.

总结 分子病理学和免疫学核心（核心2）的目标是提供一个集中的技术 基础设施，包括完成所有基于组织的 调查，包括收集组织样本、组织病理学分析、免疫监测和 维护中央数据库。核心2将作为患者标本的主要储存库， 将监督标本处理和组织病理学。将对采集的组织样本进行P01分析 项目指定的肿瘤生物标志物和利用免疫组织化学对肿瘤免疫浸润进行表型分析 用于肿瘤生物标志物和质谱细胞术以及用于免疫细胞表型分析的单细胞RNA测序。两 经验丰富的肺部病理学家和专家免疫学家将与生物信息学和 生物统计核心3和行政核心1，以确保协调采购、存档和存储 人体组织样本（尤其是临床试验样本）的无缝整合 从基于组织的研究，包括小鼠模型和临床试验，到一个安全的数据库， Cores 1和3以及P01项目的利用和进一步分析。所有基于组织的研究都将在 IRB和IACUC协议的伦理研究监督。持续沟通， 临床医生、科学家、研究护士、生物统计学家、生物信息学家和病理学家， 为所有核心活动建立的标准化作业程序将提供最佳的组织收集， 所有项目的处理、分析和数据共享。