Core 2

摘要 分子病理学和免疫学核心(核心2)的目标是提供一个集中的技术 基础设施，包括必要的材料和技术专长，以完成所有基于组织的 调查包括收集组织样本、组织病理学分析、免疫监测和 维护一个中央数据库。核心2将作为患者样本的主要储存库 将监督标本处理和组织病理学。收集的组织样本将被分析为P01 项目特异性肿瘤生物标志物和免疫组织化学肿瘤免疫浸润物的表型 用于肿瘤生物标志物和质量细胞术，以及用于免疫细胞表型的单细胞RNA测序。二 经验丰富的肺部病理学家和免疫学家专家将与生物信息学和 生物统计核心3和管理核心1，以确保协调采购、归档和存储 人体组织样本(特别是临床试验样本)和产生的数据的无缝集成 从基于组织的研究，包括从小鼠模型和临床试验，到一个安全的数据库，用于 按核心1和3以及P01项目的利用情况和进一步分析。所有以组织为基础的研究都将进行 受IRB和IACUC协议的伦理研究监督。两国之间的持续沟通 临床医生、科学家、研究护士、生物统计学家、生物信息学家和病理学家以及 为所有核心活动建立的标准化操作程序将提供最佳的组织收集， 所有项目的处理、分析和数据共享。

SUMMARY The goal of the Molecular Pathology and Immunology Core (Core 2) is to provide a centralized technical infrastructure, inclusive of materials and the technical expertise necessary to accomplish all tissue-based investigations including the collection of tissue samples, histopathological analysis, immune monitoring and maintenance of a centralized database. Core 2 will function as the main repository of patient specimens and will oversee specimen processing and histopathology. Collected tissue samples will be analyzed for P01 project specified tumor biomarkers and phenotyping of tumor immune infiltrates utilizing immunohistochemistry for tumor biomarkers and mass cytometry and single cell RNA sequencing for immune cell phenotyping. Two experienced lung pathologists and an expert immunologist will work closely with the Bioinformatics and Biostatistics Core 3 and the Administrative Core 1 to ensure coordinated procurement, archiving, and storage of human tissue samples (especially clinical trial specimens) and seamless integration of the data generated from tissue-based investigations, including from mouse models and clinical trials, into a secure, database for utilization and further analysis by Cores 1 and 3 and P01 projects. All tissue-based research will be performed with ethical research oversight from IRB and IACUC protocols. Continuous communication between the clinicians, scientists, research nurses, biostatisticians, bioinformaticians and pathologists together with established standardized operating procedures for all core activities will provide optimal tissue collection, processing, analysis, and data sharing for all projects.