Nikon Confocal Microscope for Shared Biomedical Research

项目总结/摘要 这项提案代表了一个由15名NIH资助的研究人员组成的小组的要求，他们有重叠的成像需求 获得资金购买尼康AX激光扫描共聚焦显微镜（LSM），以促进可视化， 固定细胞和组织以及活标本的三维空间组织的量化， 后者包括需要监测时间相关的变化。该系统将安装在 华盛顿大学细胞成像中心（WUCCI），一个机构范围内的共享技术资源 位于医学院。目前WUCCI中的激光扫描共聚焦显微镜已经 由具有不同需求的不同用户组大量订阅（用于模型系统成像的直立配置 和Airyscan功能，用于亚衍射极限成像）和额外的主力共焦能力， 为促进本文所述的主要和次要用户项目的工作所需。Nikon AX共焦相机 显微镜平台配置有（i）Nikon Ti 2倒置电动显微镜框架，（ii）405 nm，488 nm， 561 nm和640 nm激发激光器，（iii）高灵敏度基于滤光片的光谱检测器，（iv）各种干式 和基于浸没的物镜，以及（v）用于促进生理学的 活体标本的成像。来自医学院9个不同单位的15名研究人员将 使用这个成像平台，使广泛的基础和转化研究，旨在 了解病毒和细菌的发病机制，细胞迁移的动力学， 功能障碍、代谢稳态、糖尿病、昼夜生理学和神经退行性疾病。而 仪器已配置为满足主要用户组的特定需求，我们完全期望它 影响许多其他研究项目。这一文书的专门知识和机构支持是非同寻常的。 博士WUCCI科学总监James菲茨帕特里克和细胞生物学和生物学系主任大卫活塞博士 生理学和WUCCI顾问委员会主席，都是世界知名的细胞显微镜专家， 他们的共同领导带来了40多年的经验，在提供具有成本效益的培训和支持， 高质量的定量细胞成像，以广泛的NIH资助的用户。为了支持S10补助金 申请，该机构还将承诺100，000美元（每年20，000美元，为期五年），以确保长期 这个工具的成功。

Project Summary/Abstract This proposal represents a request from a group of 15 NIH-funded investigators with overlapping imaging needs for funding to acquire a Nikon AX Laser Scanning Confocal Microscope (LSM) to facilitate the visualization and quantification of the three-dimensional spatial organization of fixed cells and tissues as well as living specimens, with the latter including the need to monitor time-dependent changes. The system will be housed in the Washington University Center for Cellular Imaging (WUCCI), an institution-wide shared technology resource based at the School of Medicine. The current laser scanning confocal microscopes in the WUCCI are already heavily subscribed by different user groups with different needs (upright configuration for model system imaging and Airyscan capabilities for sub-diffraction limited imaging) and additional workhorse confocal capacity is needed to facilitate the work of the Major and Minor user projects described herein. The Nikon AX confocal microscope platform is configured with (i) a Nikon Ti2 inverted motorized microscope frame, (ii) 405 nm, 488 nm, 561 nm, and 640 nm excitation lasers, (iii) high-sensitivity filter-based and spectral detectors, (iv) a variety of dry and immersion-based objective lenses and (v) a stage-top incubation system to facilitate the physiological imaging of living specimens. Fifteen investigators from nine different units at the School of Medicine will make use of this imaging platform to enable a wide-range of basic and translational research studies aimed at understanding the mechanisms of viral and bacterial pathogenesis, the dynamics of cell migration, mitochondrial dysfunction, metabolic homeostasis, diabetes, circadian physiology and neurodegenerative disease. While the instrument has been configured to meet the specific needs of the major user group, we thoroughly expect it to impact many other research programs. The expertise and institutional support for this instrument are exceptional. Dr. James Fitzpatrick, the Scientific Director of WUCCI, and Dr. David Piston, the Chair of Cell Biology & Physiology and head of the WUCCI Advisory Board, are both world-renowned experts in cellular microscopy and their combined leadership brings over 40 years of experience in providing cost-efficient training and support for high quality quantitative cellular imaging to a wide range of NIH-funded users. In support of this S10 grant application, the institution will also commit $100,000 ($20,000 per year for five years) to ensure the long-term success of this instrument.