Nikon Confocal Microscope for Shared Biomedical Research
用于共享生物医学研究的尼康共焦显微镜
基本信息
- 批准号:10413403
- 负责人:
- 金额:$ 53.76万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-05-01 至 2023-04-30
- 项目状态:已结题
- 来源:
- 关键词:3-DimensionalApplications GrantsBasic ScienceBiological ModelsBiomedical ResearchCell physiologyCellsCellular biologyDiabetes MellitusEnsureFunctional ImagingFundingHeadHomeostasisImageImmersionInstitutionLasersLeadershipMetabolicMicroscopeMicroscopyMinorMonitorNeurodegenerative DisordersPathogenesisPhysiologyResearchResearch PersonnelResourcesS10 grantScanningSpecimenSystemTechnologyTimeTissuesTraining SupportTranslational ResearchUnited States National Institutes of HealthUniversitiesViralVisualizationWashingtonWorkbasecell motilitycellular imagingcircadiancost efficientdetectorexperienceimaging platformimaging systeminstrumentlensmedical schoolsmitochondrial dysfunctionprogramsresearch studysuccess
项目摘要
Project Summary/Abstract
This proposal represents a request from a group of 15 NIH-funded investigators with overlapping imaging needs
for funding to acquire a Nikon AX Laser Scanning Confocal Microscope (LSM) to facilitate the visualization and
quantification of the three-dimensional spatial organization of fixed cells and tissues as well as living specimens,
with the latter including the need to monitor time-dependent changes. The system will be housed in the
Washington University Center for Cellular Imaging (WUCCI), an institution-wide shared technology resource
based at the School of Medicine. The current laser scanning confocal microscopes in the WUCCI are already
heavily subscribed by different user groups with different needs (upright configuration for model system imaging
and Airyscan capabilities for sub-diffraction limited imaging) and additional workhorse confocal capacity is
needed to facilitate the work of the Major and Minor user projects described herein. The Nikon AX confocal
microscope platform is configured with (i) a Nikon Ti2 inverted motorized microscope frame, (ii) 405 nm, 488 nm,
561 nm, and 640 nm excitation lasers, (iii) high-sensitivity filter-based and spectral detectors, (iv) a variety of dry
and immersion-based objective lenses and (v) a stage-top incubation system to facilitate the physiological
imaging of living specimens. Fifteen investigators from nine different units at the School of Medicine will make
use of this imaging platform to enable a wide-range of basic and translational research studies aimed at
understanding the mechanisms of viral and bacterial pathogenesis, the dynamics of cell migration, mitochondrial
dysfunction, metabolic homeostasis, diabetes, circadian physiology and neurodegenerative disease. While the
instrument has been configured to meet the specific needs of the major user group, we thoroughly expect it to
impact many other research programs. The expertise and institutional support for this instrument are exceptional.
Dr. James Fitzpatrick, the Scientific Director of WUCCI, and Dr. David Piston, the Chair of Cell Biology &
Physiology and head of the WUCCI Advisory Board, are both world-renowned experts in cellular microscopy and
their combined leadership brings over 40 years of experience in providing cost-efficient training and support for
high quality quantitative cellular imaging to a wide range of NIH-funded users. In support of this S10 grant
application, the institution will also commit $100,000 ($20,000 per year for five years) to ensure the long-term
success of this instrument.
项目总结/摘要
这项提案代表了一个由15名NIH资助的研究人员组成的小组的要求,他们有重叠的成像需求
获得资金购买尼康AX激光扫描共聚焦显微镜(LSM),以促进可视化,
固定细胞和组织以及活标本的三维空间组织的量化,
后者包括需要监测时间相关的变化。该系统将安装在
华盛顿大学细胞成像中心(WUCCI),一个机构范围内的共享技术资源
位于医学院。目前WUCCI中的激光扫描共聚焦显微镜已经
由具有不同需求的不同用户组大量订阅(用于模型系统成像的直立配置
和Airyscan功能,用于亚衍射极限成像)和额外的主力共焦能力,
为促进本文所述的主要和次要用户项目的工作所需。Nikon AX共焦相机
显微镜平台配置有(i)Nikon Ti 2倒置电动显微镜框架,(ii)405 nm,488 nm,
561 nm和640 nm激发激光器,(iii)高灵敏度基于滤光片的光谱检测器,(iv)各种干式
和基于浸没的物镜,以及(v)用于促进生理学的
活体标本的成像。来自医学院九个不同单位的十五名研究人员将进行
使用这个成像平台,使广泛的基础和转化研究,旨在
了解病毒和细菌的发病机制,细胞迁移的动力学,
功能障碍、代谢稳态、糖尿病、昼夜生理学和神经退行性疾病。而
仪器已配置为满足主要用户组的特定需求,我们完全期望它
影响许多其他研究项目。这一文书的专门知识和机构支持是非同寻常的。
博士WUCCI科学总监James菲茨帕特里克和细胞生物学和生物学系主任大卫活塞博士
生理学和WUCCI顾问委员会主席,都是世界知名的细胞显微镜专家,
他们的共同领导带来了40多年的经验,在提供具有成本效益的培训和支持,
高质量的定量细胞成像,以广泛的NIH资助的用户。为了支持S10补助金
申请,该机构还将承诺100,000美元(每年20,000美元,为期五年),以确保长期
这个工具的成功。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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David W Piston其他文献
Amiloride derivatives enhance insulin release in pancreatic islets from diabetic mice
- DOI:
10.1186/1472-6823-5-9 - 发表时间:
2005-12-08 - 期刊:
- 影响因子:3.300
- 作者:
Subhadra C Gunawardana;W Steven Head;David W Piston - 通讯作者:
David W Piston
David W Piston的其他文献
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{{ truncateString('David W Piston', 18)}}的其他基金
High Sensitivity sCMOS Camera System for Transmission Electron Microscope
用于透射电子显微镜的高灵敏度 sCMOS 相机系统
- 批准号:
10414332 - 财政年份:2022
- 资助金额:
$ 53.76万 - 项目类别:
Zeiss LSM 980 Airyscan 2 Microscope for Shared Mental Health Research
用于共享心理健康研究的蔡司 LSM 980 Airyscan 2 显微镜
- 批准号:
10282117 - 财政年份:2021
- 资助金额:
$ 53.76万 - 项目类别:
Regulation of Glucagon Secretion from Pancreatic Islets
胰岛胰高血糖素分泌的调节
- 批准号:
10675668 - 财政年份:2020
- 资助金额:
$ 53.76万 - 项目类别:
Regulation of Glucagon Secretion from Pancreatic Islets
胰岛胰高血糖素分泌的调节
- 批准号:
10468865 - 财政年份:2020
- 资助金额:
$ 53.76万 - 项目类别:
Regulation of Glucagon Secretion from Pancreatic Islets
胰岛胰高血糖素分泌的调节
- 批准号:
10264101 - 财政年份:2020
- 资助金额:
$ 53.76万 - 项目类别:
Multi-dimensional Dynamics of Pancreatic Islet Cells Measured by Image Mapping diSPIM
通过图像映射 diSPIM 测量胰岛细胞的多维动力学
- 批准号:
10197901 - 财政年份:2018
- 资助金额:
$ 53.76万 - 项目类别:
Pancreatic Islets Dynamics Regulating Glucagon Secretion
胰岛动态调节胰高血糖素分泌
- 批准号:
9068608 - 财政年份:2015
- 资助金额:
$ 53.76万 - 项目类别:
Pancreatic Islets Dynamics Regulating Glucagon Secretion
胰岛动态调节胰高血糖素分泌
- 批准号:
9116182 - 财政年份:2015
- 资助金额:
$ 53.76万 - 项目类别: