Nikon Confocal Microscope for Shared Biomedical Research

用于共享生物医学研究的尼康共焦显微镜

• 批准号: 10413403
• 负责人: David W Piston
• 金额: $ 53.76万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-01 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10413403
• 关键词: 3-Dimensional; Applications Grants; Basic Science; Biological Models; Biomedical Research; Cell physiology; Cells; Cellular biology; Diabetes Mellitus; Ensure; Functional Imaging; Funding; Head; Homeostasis; Image; Immersion; Institution; Lasers; Leadership; Metabolic; Microscope; Microscopy; Minor; Monitor; Neurodegenerative Disorders; Pathogenesis; Physiology; Research; Research Personnel; Resources; S10 grant; Scanning; Specimen; System; Technology; Time; Tissues; Training Support; Translational Research; United States National Institutes of Health; Universities; Viral; Visualization; Washington; Work; base; cell motility; cellular imaging; circadian; cost efficient; detector; experience; imaging platform; imaging system; instrument; lens; medical schools; mitochondrial dysfunction; programs; research study; success

Project Summary/Abstract This proposal represents a request from a group of 15 NIH-funded investigators with overlapping imaging needs for funding to acquire a Nikon AX Laser Scanning Confocal Microscope (LSM) to facilitate the visualization and quantification of the three-dimensional spatial organization of fixed cells and tissues as well as living specimens, with the latter including the need to monitor time-dependent changes. The system will be housed in the Washington University Center for Cellular Imaging (WUCCI), an institution-wide shared technology resource based at the School of Medicine. The current laser scanning confocal microscopes in the WUCCI are already heavily subscribed by different user groups with different needs (upright configuration for model system imaging and Airyscan capabilities for sub-diffraction limited imaging) and additional workhorse confocal capacity is needed to facilitate the work of the Major and Minor user projects described herein. The Nikon AX confocal microscope platform is configured with (i) a Nikon Ti2 inverted motorized microscope frame, (ii) 405 nm, 488 nm, 561 nm, and 640 nm excitation lasers, (iii) high-sensitivity filter-based and spectral detectors, (iv) a variety of dry and immersion-based objective lenses and (v) a stage-top incubation system to facilitate the physiological imaging of living specimens. Fifteen investigators from nine different units at the School of Medicine will make use of this imaging platform to enable a wide-range of basic and translational research studies aimed at understanding the mechanisms of viral and bacterial pathogenesis, the dynamics of cell migration, mitochondrial dysfunction, metabolic homeostasis, diabetes, circadian physiology and neurodegenerative disease. While the instrument has been configured to meet the specific needs of the major user group, we thoroughly expect it to impact many other research programs. The expertise and institutional support for this instrument are exceptional. Dr. James Fitzpatrick, the Scientific Director of WUCCI, and Dr. David Piston, the Chair of Cell Biology & Physiology and head of the WUCCI Advisory Board, are both world-renowned experts in cellular microscopy and their combined leadership brings over 40 years of experience in providing cost-efficient training and support for high quality quantitative cellular imaging to a wide range of NIH-funded users. In support of this S10 grant application, the institution will also commit $100,000 ($20,000 per year for five years) to ensure the long-term success of this instrument.

项目摘要/摘要 这项提案代表了由15名NIH资助的具有重叠成像需求的调查人员提出的请求 为购买尼康AX激光扫描共聚焦显微镜(LSM)提供资金，以促进可视化和 对固定细胞和组织以及活标本的三维空间组织进行量化， 后者包括监控依赖于时间的变化的需要。该系统将安装在 华盛顿大学细胞成像中心(WUCCI)，一个机构范围内共享的技术资源 在医学院工作。目前WUCCI的激光扫描共聚焦显微镜已经 由具有不同需求的不同用户组大量订阅(模型系统映像的直立式配置 和用于次衍射限制成像的AiryScan能力)和额外的主要共焦能力是 为促进本文所述的主要用户项目和次要用户项目的工作所需。尼康AX共焦 显微镜平台配置有(I)尼康TI2倒置电动显微镜框架，(Ii)405 nm、488 nm， 561 nm和640 nm激发激光器，(Iii)高灵敏度滤光式和光谱探测器，(Iv)各种干式 和基于浸泡的物镜，以及(V)台顶孵化系统，以促进生理 对活体标本进行成像。来自医学院9个不同单位的15名调查人员将 利用这一成像平台实现广泛的基础和转化性研究 了解病毒和细菌的致病机制，细胞迁移的动力学，线粒体 功能障碍、代谢稳态、糖尿病、昼夜生理学和神经退行性疾病。而当 仪器已配置为满足主要用户群体的特定需求，我们完全希望它能 影响了许多其他研究项目。对这一文书的专门知识和机构支持是特殊的。 WUCCI科学主任James Fitzpatrick博士和细胞生物学与 生理学和WUCCI咨询委员会主席，都是世界知名的细胞显微镜专家和 他们的共同领导带来了在提供经济高效的培训和支持方面的40多年经验 为NIH资助的广泛用户提供高质量的定量细胞成像。以支持这项中十助学金 申请，该机构还将承诺10万美元(五年内每年20,000美元)，以确保长期 这件乐器的成功。