Nikon Confocal Microscope for Shared Biomedical Research
用于共享生物医学研究的尼康共焦显微镜
基本信息
- 批准号:10413403
- 负责人:
- 金额:$ 53.76万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-05-01 至 2023-04-30
- 项目状态:已结题
- 来源:
- 关键词:3-DimensionalApplications GrantsBasic ScienceBiological ModelsBiomedical ResearchCell physiologyCellsCellular biologyDiabetes MellitusEnsureFunctional ImagingFundingHeadHomeostasisImageImmersionInstitutionLasersLeadershipMetabolicMicroscopeMicroscopyMinorMonitorNeurodegenerative DisordersPathogenesisPhysiologyResearchResearch PersonnelResourcesS10 grantScanningSpecimenSystemTechnologyTimeTissuesTraining SupportTranslational ResearchUnited States National Institutes of HealthUniversitiesViralVisualizationWashingtonWorkbasecell motilitycellular imagingcircadiancost efficientdetectorexperienceimaging platformimaging systeminstrumentlensmedical schoolsmitochondrial dysfunctionprogramsresearch studysuccess
项目摘要
Project Summary/Abstract
This proposal represents a request from a group of 15 NIH-funded investigators with overlapping imaging needs
for funding to acquire a Nikon AX Laser Scanning Confocal Microscope (LSM) to facilitate the visualization and
quantification of the three-dimensional spatial organization of fixed cells and tissues as well as living specimens,
with the latter including the need to monitor time-dependent changes. The system will be housed in the
Washington University Center for Cellular Imaging (WUCCI), an institution-wide shared technology resource
based at the School of Medicine. The current laser scanning confocal microscopes in the WUCCI are already
heavily subscribed by different user groups with different needs (upright configuration for model system imaging
and Airyscan capabilities for sub-diffraction limited imaging) and additional workhorse confocal capacity is
needed to facilitate the work of the Major and Minor user projects described herein. The Nikon AX confocal
microscope platform is configured with (i) a Nikon Ti2 inverted motorized microscope frame, (ii) 405 nm, 488 nm,
561 nm, and 640 nm excitation lasers, (iii) high-sensitivity filter-based and spectral detectors, (iv) a variety of dry
and immersion-based objective lenses and (v) a stage-top incubation system to facilitate the physiological
imaging of living specimens. Fifteen investigators from nine different units at the School of Medicine will make
use of this imaging platform to enable a wide-range of basic and translational research studies aimed at
understanding the mechanisms of viral and bacterial pathogenesis, the dynamics of cell migration, mitochondrial
dysfunction, metabolic homeostasis, diabetes, circadian physiology and neurodegenerative disease. While the
instrument has been configured to meet the specific needs of the major user group, we thoroughly expect it to
impact many other research programs. The expertise and institutional support for this instrument are exceptional.
Dr. James Fitzpatrick, the Scientific Director of WUCCI, and Dr. David Piston, the Chair of Cell Biology &
Physiology and head of the WUCCI Advisory Board, are both world-renowned experts in cellular microscopy and
their combined leadership brings over 40 years of experience in providing cost-efficient training and support for
high quality quantitative cellular imaging to a wide range of NIH-funded users. In support of this S10 grant
application, the institution will also commit $100,000 ($20,000 per year for five years) to ensure the long-term
success of this instrument.
项目摘要/摘要
这项提案代表了由15名NIH资助的具有重叠成像需求的调查人员提出的请求
为购买尼康AX激光扫描共聚焦显微镜(LSM)提供资金,以促进可视化和
对固定细胞和组织以及活标本的三维空间组织进行量化,
后者包括监控依赖于时间的变化的需要。该系统将安装在
华盛顿大学细胞成像中心(WUCCI),一个机构范围内共享的技术资源
在医学院工作。目前WUCCI的激光扫描共聚焦显微镜已经
由具有不同需求的不同用户组大量订阅(模型系统映像的直立式配置
和用于次衍射限制成像的AiryScan能力)和额外的主要共焦能力是
为促进本文所述的主要用户项目和次要用户项目的工作所需。尼康AX共焦
显微镜平台配置有(I)尼康TI2倒置电动显微镜框架,(Ii)405 nm、488 nm,
561 nm和640 nm激发激光器,(Iii)高灵敏度滤光式和光谱探测器,(Iv)各种干式
和基于浸泡的物镜,以及(V)台顶孵化系统,以促进生理
对活体标本进行成像。来自医学院9个不同单位的15名调查人员将
利用这一成像平台实现广泛的基础和转化性研究
了解病毒和细菌的致病机制,细胞迁移的动力学,线粒体
功能障碍、代谢稳态、糖尿病、昼夜生理学和神经退行性疾病。而当
仪器已配置为满足主要用户群体的特定需求,我们完全希望它能
影响了许多其他研究项目。对这一文书的专门知识和机构支持是特殊的。
WUCCI科学主任James Fitzpatrick博士和细胞生物学与
生理学和WUCCI咨询委员会主席,都是世界知名的细胞显微镜专家和
他们的共同领导带来了在提供经济高效的培训和支持方面的40多年经验
为NIH资助的广泛用户提供高质量的定量细胞成像。以支持这项中十助学金
申请,该机构还将承诺10万美元(五年内每年20,000美元),以确保长期
这件乐器的成功。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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David W Piston其他文献
Amiloride derivatives enhance insulin release in pancreatic islets from diabetic mice
- DOI:
10.1186/1472-6823-5-9 - 发表时间:
2005-12-08 - 期刊:
- 影响因子:3.300
- 作者:
Subhadra C Gunawardana;W Steven Head;David W Piston - 通讯作者:
David W Piston
David W Piston的其他文献
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{{ truncateString('David W Piston', 18)}}的其他基金
High Sensitivity sCMOS Camera System for Transmission Electron Microscope
用于透射电子显微镜的高灵敏度 sCMOS 相机系统
- 批准号:
10414332 - 财政年份:2022
- 资助金额:
$ 53.76万 - 项目类别:
Zeiss LSM 980 Airyscan 2 Microscope for Shared Mental Health Research
用于共享心理健康研究的蔡司 LSM 980 Airyscan 2 显微镜
- 批准号:
10282117 - 财政年份:2021
- 资助金额:
$ 53.76万 - 项目类别:
Regulation of Glucagon Secretion from Pancreatic Islets
胰岛胰高血糖素分泌的调节
- 批准号:
10675668 - 财政年份:2020
- 资助金额:
$ 53.76万 - 项目类别:
Regulation of Glucagon Secretion from Pancreatic Islets
胰岛胰高血糖素分泌的调节
- 批准号:
10468865 - 财政年份:2020
- 资助金额:
$ 53.76万 - 项目类别:
Regulation of Glucagon Secretion from Pancreatic Islets
胰岛胰高血糖素分泌的调节
- 批准号:
10264101 - 财政年份:2020
- 资助金额:
$ 53.76万 - 项目类别:
Multi-dimensional Dynamics of Pancreatic Islet Cells Measured by Image Mapping diSPIM
通过图像映射 diSPIM 测量胰岛细胞的多维动力学
- 批准号:
10197901 - 财政年份:2018
- 资助金额:
$ 53.76万 - 项目类别:
Pancreatic Islets Dynamics Regulating Glucagon Secretion
胰岛动态调节胰高血糖素分泌
- 批准号:
9068608 - 财政年份:2015
- 资助金额:
$ 53.76万 - 项目类别:
Pancreatic Islets Dynamics Regulating Glucagon Secretion
胰岛动态调节胰高血糖素分泌
- 批准号:
9116182 - 财政年份:2015
- 资助金额:
$ 53.76万 - 项目类别: