Cellular Imaging Core

项目摘要：细胞成像核心 先进的细胞显微镜是生物学研究的有力工具，在 对疾病发病机制的研究，最终可能转化为新的治疗方法 风湿病。成像技术在过去的十年中迅速发展，带来了改进 在分辨率、灵敏度和速度方面，这为研究创造了根本的新机会 在活细胞和动物中，跨越许多数量级的实时过程。同时， 日益复杂的设备的成本是巨大的，而有效使用、维护、 而开发这种设备在大多数实验室中并不常见。因此，两国之间存在着很大的差距 这些强大工具的可用性以及调查人员有效获取和使用这些工具的能力。这个 细胞成像核心(CIC)最初是为了响应华盛顿大学的反馈而创建的 风湿病研究资源中心(WU-RDRRC)成员利用重要的 机构投资于新成立的华盛顿大学细胞成像中心(WUCCI)和 提供一种综合的方法来研究细胞和组织的结构和动态行为 风湿病相关模型系统。核心的总体目标是提供访问和 在使用先进的细胞显微镜工具方面提供技术支持，以加快步伐，扩大范围，以及 提高风湿病研究效率。核心服务满足以下独特要求 众多研究人员从事广泛的基础研究和翻译研究，吸引了新的研究人员 进入风湿病研究领域。重要的是，用户受益于 核心主任和技术人员在咨询试验设计和技术方面所花费的时间 数据的解释。在过去的资助周期中，该核心服务于28个WU-RDRRC调查员研究 实验室，在核心充值方面花费了520,699美元，约占所有WUCCI的12% 活动。WU-RDRRC的14个调查实验室通过WU-RDRRC获得了小额赠款- RDRRC影像核心奖学金计划，其中5人是初级教员。考虑到广泛的用法 在过去的供资周期中对核心进行了评估，并建立了新的成像技术(例如，冷冻EM和 点阵光板)、样品制备服务(例如组织清理)和分析方法(例如基于人工智能的 IHC和IF图像分析)，预计CIC服务请求将继续保持强劲，因为 对先进的显微成像和图像分析方法的迫切需求，从而加速了 WU-RDRRC成员在下一个资助周期的研究努力。

PROJECT SUMMARY: Cellular Imaging Core Advanced cellular microscopy is a powerful tool for biological research and has an important role to play in the study of disease pathogenesis, which may translate eventually to novel treatment approaches for rheumatic diseases. Imaging technology has evolved rapidly over the last decade leading to improvements in resolution, sensitivity and speed which have created fundamentally new opportunities for studying processes across many orders of magnitude and in real-time in living cells and animals. At the same time, the costs of increasingly sophisticated equipment are substantial and the expertise to efficiently use, maintain, and develop this equipment is not common in most labs. There is therefore a significant gap between the availability of these powerful tools and the ability of investigators to access and use them efficiently. The Cellular Imaging Core (CIC) was originally created in response to feedback from Washington University Rheumatic Diseases Research Resource-based Center (WU-RDRRC) members to leverage the significant institutional investment in the newly created Washington University Center for Cellular Imaging (WUCCI) and provide an integrated approach to investigate the structure and dynamic behavior of cells and tissues in rheumatic disease-related model systems. The overall objective of the core is to provide access to and technical support in using advanced cellular microscopy tools to accelerate the pace, expand the scope, and improve efficiency of rheumatic diseases research. The core services meet the unique requirements of numerous investigators over a wide range of basic and translational research, attracting new investigators into rheumatic diseases research areas. Importantly the users benefit from the in-depth technical expertise of the Core Director and technical staff in the time spent in consultation for experimental design and interpretation of data. During the past funding cycle, the core served 28 WU-RDRRC investigator research laboratories, who expensed $520,699 in core recharges, which represented approximately 12% of all WUCCI activity. Fourteen WU-RDRRC investigator laboratories received micro-grant funding through the WU- RDRRC Imaging Core Scholarship program, 5 of which were junior faculty members. Given the broad usage of core over the past funding cycle, and the establishment of new imaging technologies (e.g. cryoEM and lattice lightsheet), sample preparation services (e.g. tissue clearing), and analysis approaches (e.g. AI-based IHC and IF image analysis), it is anticipated that requests for CIC services will continue to be robust given the overwhelming need for advanced microscopic imaging and image analysis approaches, thus accelerating the research endeavors of WU-RDRRC members throughout the next funding cycle.