Imaging Cells during Behavior Core
行为核心期间的细胞成像
基本信息
- 批准号:10413186
- 负责人:
- 金额:$ 38.27万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-07-01 至 2025-05-31
- 项目状态:未结题
- 来源:
- 关键词:3-DimensionalAddressAgingAnimalsBehaviorBrainBrain imagingCerebral cortexChronicCognitionCommunitiesComplementComputer softwareDataDecision MakingDevelopmentDiseaseDorsalElementsEngineeringEquipmentFailureFiberFree WillHeadHuman ResourcesImageImage EnhancementImaging DeviceImaging TechniquesImaging technologyIndividualLaboratoriesMagnetic Resonance ImagingMagnetismMethodsMicroscopeMinnesotaModelingModernizationMonitorMovementMusNational Institute of Drug AbuseNervous System PhysiologyNeuronsNeurosciencesNeurosciences ResearchOpticsPatternPerceptionPhotometryPhotonsProcessProductivityRattusRelapseResearchResearch InfrastructureResearch PersonnelResidual stateResolutionResourcesServicesSpeedStructureSurfaceTechniquesTechnologyTimeTrainingUniversitiesVisualizationWorkaddictionanalytical toolcellular imagingcohesioncomputerized toolscostdesignexperimental studyimaging capabilitiesimaging modalityimprovedin vivoin vivo imaginglearning networkminiaturizemouse modelnervous system imagingneural circuitnovel therapeuticsoptical imagingprogramsrelating to nervous systemspatiotemporaltool
项目摘要
PROJECT SUMMARY: Imaging Cells during Behavior Core
Addiction is a chronic, relapsing disorder due to perturbations in neural circuits. To better understand the
underlying pathophysiological processes occurring in addiction and the actions of new therapies, there are
pressing needs to monitor neural activity and structure during addiction and relapse. Visualization of nervous
system function and structure in the intact, behaving animal is a powerful approach to meet these needs. Recent
advances in genetically encoded Ca2+ indicators and imaging instruments have greatly expanded the
opportunities for imaging the nervous system at work. Concurrently, there is a revolution in the use of high-speed
video cameras combined with sophisticated computational tools to monitor and quantify behavior.
Many labs at the University of Minnesota (UMN) lack the equipment and expertise to perform neural imaging
during behavior. Nor is it possible for most investigators to keep up with the rapid changes in optical tools, either
in the engineering or application domains. The Imaging Cells during Behavior Core (ICBC) is designed to allow
addiction researchers and the wider neuroscience community access to modern techniques to image brain
function and structure during behavior. Providing up-to-date imaging techniques, engineering and manufacturing
support, as well as computational tools, will not only reduce the time and costs needed to set up experiments
and process data, but also lower the entry barriers for both new and established investigators. The ICBC’s
engineering, experimental, and analytical expertise will allow labs to be at the forefront of imaging technology.
Aim 1 is to provide resources, expertise and training in three in vivo imaging technologies used in behaving
animals: 1) head-mounted miniaturized microscopes for cellular level imaging at the surface and in deeper brain
structures; 2) wide field-of-view (FOV) imaging of the cerebral cortex at both the mesoscopic and cellular levels;
and 3) fiber photometry to monitor the activity of genetically defined elements in neural circuits. UMN
investigators deemed these technologies critical to understanding how neural circuits change in addiction. The
ICBC will provide sophisticated hardware and software to monitor and analyze behavior in head-fixed and freely
moving animals and the engineering and analytical expertise to build and use these in vivo imaging technologies.
Aim 2 extends and adds new imaging capabilities. Many UMN addiction researchers use rats, but most neural
imaging techniques were developed for mice. Therefore, we will extend our imaging tools to the rat. In addition,
Aim 2 will combine fiber photometry with wide FOV cortical imaging, enhance wide FOV imaging to access
deeper cortical structures, and make wide FOV imaging compatible with magnetic resonance imaging. ICBC
personnel will work closely with investigators to employ these improvements to advance their research programs.
In summary, the ICBC will provide the research infrastructure, expertise and training to allow neuroscientists to
image neuronal activity and neural circuitry during behavior. Providing these tools will enhance the productivity,
quality, and impact of addiction-related and neuroscience research in general at the UMN.
项目总结:行为核心期间的细胞成像
成瘾是一种慢性复发性疾病,由于神经回路的扰动。更好地了解
成瘾的潜在病理生理过程和新疗法的作用,
迫切需要监测成瘾和复发期间的神经活动和结构。神经可视化
系统功能和结构的完整,行为的动物是一个强大的方法来满足这些需求。最近
基因编码的Ca 2+指示剂和成像仪器的进步极大地扩展了
在工作中对神经系统进行成像的机会。与此同时,在高速公路的使用方面也发生了一场革命。
视频摄像机结合复杂的计算工具来监控和量化行为。
明尼苏达大学的许多实验室缺乏进行神经成像的设备和专业知识
在行为上。大多数研究人员也不可能跟上光学工具的快速变化,
在工程或应用领域。行为核心期间的成像细胞(ICBC)旨在允许
成瘾研究人员和更广泛的神经科学界获得现代技术来成像大脑
行为过程中的功能和结构。提供最新的成像技术、工程和制造
支持以及计算工具不仅可以减少建立实验所需的时间和成本,
和处理数据,但也降低了新的和已建立的调查人员的进入门槛。工行的
工程、实验和分析专业知识将使实验室处于成像技术的最前沿。
目标1是提供三种在体成像技术的资源、专业知识和培训,
动物:1)头戴式微型显微镜,用于大脑表面和深层的细胞水平成像
2)在介观和细胞水平上对大脑皮层进行宽视场(FOV)成像;
3)纤维光度测定法,用于监测神经回路中遗传限定元素的活性。UMN
研究人员认为,这些技术对于了解成瘾中神经回路的变化至关重要。的
工商银行将提供先进的硬件和软件来监测和分析头部固定和自由的行为
移动动物和工程和分析专业知识,以建立和使用这些在体内成像技术。
Aim 2扩展并增加了新的成像功能。许多UMN成瘾研究人员使用大鼠,但大多数神经
为小鼠开发了成像技术。因此,我们将我们的成像工具扩展到大鼠。此外,本发明还提供了一种方法,
目标2将联合收割机纤维光度测定与宽FOV皮质成像结合,增强宽FOV成像,以进入
更深的皮质结构,并使宽FOV成像与磁共振成像兼容。工行
研究人员将与研究人员密切合作,利用这些改进来推进他们的研究计划。
总之,ICBC将提供研究基础设施,专业知识和培训,使神经科学家能够
对行为过程中神经元活动和神经回路进行成像。提供这些工具将提高生产力,
质量和成瘾相关的影响和神经科学研究一般在UMN。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('TIMOTHY J EBNER', 18)}}的其他基金
Robot assisted brain-wide neural recordings and comprehensive behavioral monitoring in freely behaving mice
机器人辅助自由行为小鼠的全脑神经记录和全面行为监测
- 批准号:
10401192 - 财政年份:2022
- 资助金额:
$ 38.27万 - 项目类别:
Using a novel mTBI model to investigate phosphorylation dependent common mechanisms in tauopathies
使用新型 mTBI 模型研究 tau蛋白病的磷酸化依赖性常见机制
- 批准号:
10369078 - 财政年份:2022
- 资助金额:
$ 38.27万 - 项目类别:
Using a novel mTBI model to investigate phosphorylation dependent common mechanisms in tauopathies
使用新型 mTBI 模型研究 tau蛋白病的磷酸化依赖性常见机制
- 批准号:
10625988 - 财政年份:2022
- 资助金额:
$ 38.27万 - 项目类别:
Full human gene-replacement mouse models of ADRDs
ADRD 的完整人类基因替代小鼠模型
- 批准号:
10464809 - 财政年份:2019
- 资助金额:
$ 38.27万 - 项目类别:
Full human gene-replacement mouse models of ADRDs
ADRD 的完整人类基因替代小鼠模型
- 批准号:
9893130 - 财政年份:2019
- 资助金额:
$ 38.27万 - 项目类别:
University of Minnesota Summer Research in Neuroscience
明尼苏达大学神经科学暑期研究
- 批准号:
9021695 - 财政年份:2013
- 资助金额:
$ 38.27万 - 项目类别:
University of Minnesota Summer Research in Neuroscience
明尼苏达大学神经科学暑期研究
- 批准号:
8518798 - 财政年份:2013
- 资助金额:
$ 38.27万 - 项目类别:
University of Minnesota Summer Research in Neuroscience
明尼苏达大学神经科学暑期研究
- 批准号:
8624727 - 财政年份:2013
- 资助金额:
$ 38.27万 - 项目类别:
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