Imaging Cells during Behavior Core

项目总结：行为核心期间的细胞成像 成瘾是一种慢性复发性疾病，由于神经回路的扰动。更好地了解 成瘾的潜在病理生理过程和新疗法的作用， 迫切需要监测成瘾和复发期间的神经活动和结构。神经可视化 系统功能和结构的完整，行为的动物是一个强大的方法来满足这些需求。最近 基因编码的Ca 2+指示剂和成像仪器的进步极大地扩展了 在工作中对神经系统进行成像的机会。与此同时，在高速公路的使用方面也发生了一场革命。 视频摄像机结合复杂的计算工具来监控和量化行为。 明尼苏达大学的许多实验室缺乏进行神经成像的设备和专业知识 在行为上。大多数研究人员也不可能跟上光学工具的快速变化， 在工程或应用领域。行为核心期间的成像细胞（ICBC）旨在允许 成瘾研究人员和更广泛的神经科学界获得现代技术来成像大脑 行为过程中的功能和结构。提供最新的成像技术、工程和制造 支持以及计算工具不仅可以减少建立实验所需的时间和成本， 和处理数据，但也降低了新的和已建立的调查人员的进入门槛。工行的 工程、实验和分析专业知识将使实验室处于成像技术的最前沿。 目标1是提供三种在体成像技术的资源、专业知识和培训， 动物：1）头戴式微型显微镜，用于大脑表面和深层的细胞水平成像 2）在介观和细胞水平上对大脑皮层进行宽视场（FOV）成像; 3）纤维光度测定法，用于监测神经回路中遗传限定元素的活性。UMN 研究人员认为，这些技术对于了解成瘾中神经回路的变化至关重要。的 工商银行将提供先进的硬件和软件来监测和分析头部固定和自由的行为 移动动物和工程和分析专业知识，以建立和使用这些在体内成像技术。 Aim 2扩展并增加了新的成像功能。许多UMN成瘾研究人员使用大鼠，但大多数神经 为小鼠开发了成像技术。因此，我们将我们的成像工具扩展到大鼠。此外，本发明还提供了一种方法， 目标2将联合收割机纤维光度测定与宽FOV皮质成像结合，增强宽FOV成像，以进入 更深的皮质结构，并使宽FOV成像与磁共振成像兼容。工行 研究人员将与研究人员密切合作，利用这些改进来推进他们的研究计划。 总之，ICBC将提供研究基础设施，专业知识和培训，使神经科学家能够 对行为过程中神经元活动和神经回路进行成像。提供这些工具将提高生产力， 质量和成瘾相关的影响和神经科学研究一般在UMN。

PROJECT SUMMARY: Imaging Cells during Behavior Core Addiction is a chronic, relapsing disorder due to perturbations in neural circuits. To better understand the underlying pathophysiological processes occurring in addiction and the actions of new therapies, there are pressing needs to monitor neural activity and structure during addiction and relapse. Visualization of nervous system function and structure in the intact, behaving animal is a powerful approach to meet these needs. Recent advances in genetically encoded Ca2+ indicators and imaging instruments have greatly expanded the opportunities for imaging the nervous system at work. Concurrently, there is a revolution in the use of high-speed video cameras combined with sophisticated computational tools to monitor and quantify behavior. Many labs at the University of Minnesota (UMN) lack the equipment and expertise to perform neural imaging during behavior. Nor is it possible for most investigators to keep up with the rapid changes in optical tools, either in the engineering or application domains. The Imaging Cells during Behavior Core (ICBC) is designed to allow addiction researchers and the wider neuroscience community access to modern techniques to image brain function and structure during behavior. Providing up-to-date imaging techniques, engineering and manufacturing support, as well as computational tools, will not only reduce the time and costs needed to set up experiments and process data, but also lower the entry barriers for both new and established investigators. The ICBC’s engineering, experimental, and analytical expertise will allow labs to be at the forefront of imaging technology. Aim 1 is to provide resources, expertise and training in three in vivo imaging technologies used in behaving animals: 1) head-mounted miniaturized microscopes for cellular level imaging at the surface and in deeper brain structures; 2) wide field-of-view (FOV) imaging of the cerebral cortex at both the mesoscopic and cellular levels; and 3) fiber photometry to monitor the activity of genetically defined elements in neural circuits. UMN investigators deemed these technologies critical to understanding how neural circuits change in addiction. The ICBC will provide sophisticated hardware and software to monitor and analyze behavior in head-fixed and freely moving animals and the engineering and analytical expertise to build and use these in vivo imaging technologies. Aim 2 extends and adds new imaging capabilities. Many UMN addiction researchers use rats, but most neural imaging techniques were developed for mice. Therefore, we will extend our imaging tools to the rat. In addition, Aim 2 will combine fiber photometry with wide FOV cortical imaging, enhance wide FOV imaging to access deeper cortical structures, and make wide FOV imaging compatible with magnetic resonance imaging. ICBC personnel will work closely with investigators to employ these improvements to advance their research programs. In summary, the ICBC will provide the research infrastructure, expertise and training to allow neuroscientists to image neuronal activity and neural circuitry during behavior. Providing these tools will enhance the productivity, quality, and impact of addiction-related and neuroscience research in general at the UMN.