Console and Probe Upgrade for a 700 MHz NMR Spectrometer

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The Iowa State University Biomolecular NMR Facility is seeking funding to replace and upgrade the console, cryoprobe and accessories for its 700 MHz Nuclear Magnetic Resonance (NMR) spectrometer. This instrument serves a vibrant group of research faculty at Iowa State University (ISU) that pursue NIH funded research programs tackling questions related to biomolecular structures and complexes, natural products, protein disorder/aggregation, metabolomics, and small molecule drug discovery. The requested upgrade will dramatically increase access to high field NMR spectrometers at ISU allowing the long-term research objectives of our faculty to be realized. As the community of structural biologists at ISU has expanded rapidly over the past decade, the Biomolecular NMR Facility has experienced increased demand for NMR time. The specific request includes replacing all console electronics, all magnet accessories such as shims, the complete cryoprobe system and adding an autosampler. The original 54 mm bore 16.5 T Bruker UltraShield superconducting magnet will be maintained. It is typical for NMR magnets to be serviceable for several decades (much longer than consoles). The existing 15-year-old console has reached the end of its useful life as an instrument for state-of-the-art biomolecular NMR research. The need for repair downtime on the 700 MHz instrument has increased steadily in recent years while at the same time the manufacturer no longer guarantees the availability of replacement parts. Even when in good operational condition, the performance of the spectrometer is not up to the standards of modern methodologies, particularly the nuclear relaxation dispersion experiments required by ISU researchers. Thus, a successful upgrade of the 700 MHz NMR spectrometer at ISU will enhance ongoing research projects related to human health by providing greater access to high field instrumentation as well as additional capabilities currently not available on the 700 MHz instrument.