Console and Probe Upgrade for a 700 MHz NMR Spectrometer

700 MHz NMR 波谱仪的控制台和探头升级

• 批准号: 10415275
• 负责人: AMY H ANDREOTTI
• 金额: $ 101.69万
• 依托单位: IOWA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-15 至 2023-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10415275
• 关键词: 15 year old; Biomolecular Nuclear Magnetic Resonance; Communities; Complex; Disease; Electronics; Faculty; Funding; Health; Human; Iowa; Life; Manufacturer Name; Methodology; Modernization; Natural Products; Nuclear; Nuclear Magnetic Resonance; Performance; Proteins; Relaxation; Research; Research Personnel; Research Project Grants; Services; Structural Biologist; Structure; System; Time; United States National Institutes of Health; Universities; drug discovery; experience; experimental study; faculty research; instrument; instrumentation; metabolomics; programs; repaired; small molecule

The Iowa State University Biomolecular NMR Facility is seeking funding to replace and upgrade the console, cryoprobe and accessories for its 700 MHz Nuclear Magnetic Resonance (NMR) spectrometer. This instrument serves a vibrant group of research faculty at Iowa State University (ISU) that pursue NIH funded research programs tackling questions related to biomolecular structures and complexes, natural products, protein disorder/aggregation, metabolomics, and small molecule drug discovery. The requested upgrade will dramatically increase access to high field NMR spectrometers at ISU allowing the long-term research objectives of our faculty to be realized. As the community of structural biologists at ISU has expanded rapidly over the past decade, the Biomolecular NMR Facility has experienced increased demand for NMR time. The specific request includes replacing all console electronics, all magnet accessories such as shims, the complete cryoprobe system and adding an autosampler. The original 54 mm bore 16.5 T Bruker UltraShield superconducting magnet will be maintained. It is typical for NMR magnets to be serviceable for several decades (much longer than consoles). The existing 15-year-old console has reached the end of its useful life as an instrument for state-of-the-art biomolecular NMR research. The need for repair downtime on the 700 MHz instrument has increased steadily in recent years while at the same time the manufacturer no longer guarantees the availability of replacement parts. Even when in good operational condition, the performance of the spectrometer is not up to the standards of modern methodologies, particularly the nuclear relaxation dispersion experiments required by ISU researchers. Thus, a successful upgrade of the 700 MHz NMR spectrometer at ISU will enhance ongoing research projects related to human health by providing greater access to high field instrumentation as well as additional capabilities currently not available on the 700 MHz instrument.

爱荷华州立大学生物分子核磁共振设施正在寻求资金来更换和升级 700 MHz核磁共振的控制台、低温探头和附件 分光计。这台仪器为爱荷华州立大学充满活力的研究人员服务 继续NIH资助的研究项目的大学(ISU)解决与 生物分子结构和复合体，天然产物，蛋白质无序/聚集， 代谢组学和小分子药物发现。请求的升级将极大地 增加对ISU高场核磁共振波谱仪的使用，从而允许长期研究 我们教职员工的目标有待实现。正如ISU的结构生物学家社区所做的那样 在过去的十年里，生物分子核磁共振设备迅速扩张，经历了 对核磁共振时间的需求增加。具体要求包括更换所有控制台电子设备， 所有磁铁配件，如垫片，完整的冷冻探头系统，并增加了一个 自动取样器。最初的54毫米口径16.5T布鲁克超屏蔽超导磁体将 会得到维护。核磁共振磁体通常可以使用几十年(更长时间 而非游戏机)。现有的已有15年历史的游戏机作为一种 先进的生物分子核磁共振研究仪器。需要维修的停机时间 700 MHz仪器近年来稳步增长，同时 制造商不再保证更换部件的可用性。即使是在好的时候 运行条件下，光谱仪的性能不符合标准 现代方法，特别是所需的核弛豫色散实验 ISU研究人员。因此，执行支助股700兆赫核磁共振波谱仪的成功升级将 加强正在进行的与人类健康相关的研究项目，提供更多的机会 现场测试以及700 MHz上目前无法提供的其他功能 乐器。