Console and Probe Upgrade for a 700 MHz NMR Spectrometer

700 MHz NMR 波谱仪的控制台和探头升级

• 批准号: 10415275
• 负责人: AMY H ANDREOTTI
• 金额: $ 101.69万
• 依托单位: IOWA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-15 至 2023-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10415275
• 关键词: 15 year old; Biomolecular Nuclear Magnetic Resonance; Communities; Complex; Disease; Electronics; Faculty; Funding; Health; Human; Iowa; Life; Manufacturer Name; Methodology; Modernization; Natural Products; Nuclear; Nuclear Magnetic Resonance; Performance; Proteins; Relaxation; Research; Research Personnel; Research Project Grants; Services; Structural Biologist; Structure; System; Time; United States National Institutes of Health; Universities; drug discovery; experience; experimental study; faculty research; instrument; instrumentation; metabolomics; programs; repaired; small molecule

The Iowa State University Biomolecular NMR Facility is seeking funding to replace and upgrade the console, cryoprobe and accessories for its 700 MHz Nuclear Magnetic Resonance (NMR) spectrometer. This instrument serves a vibrant group of research faculty at Iowa State University (ISU) that pursue NIH funded research programs tackling questions related to biomolecular structures and complexes, natural products, protein disorder/aggregation, metabolomics, and small molecule drug discovery. The requested upgrade will dramatically increase access to high field NMR spectrometers at ISU allowing the long-term research objectives of our faculty to be realized. As the community of structural biologists at ISU has expanded rapidly over the past decade, the Biomolecular NMR Facility has experienced increased demand for NMR time. The specific request includes replacing all console electronics, all magnet accessories such as shims, the complete cryoprobe system and adding an autosampler. The original 54 mm bore 16.5 T Bruker UltraShield superconducting magnet will be maintained. It is typical for NMR magnets to be serviceable for several decades (much longer than consoles). The existing 15-year-old console has reached the end of its useful life as an instrument for state-of-the-art biomolecular NMR research. The need for repair downtime on the 700 MHz instrument has increased steadily in recent years while at the same time the manufacturer no longer guarantees the availability of replacement parts. Even when in good operational condition, the performance of the spectrometer is not up to the standards of modern methodologies, particularly the nuclear relaxation dispersion experiments required by ISU researchers. Thus, a successful upgrade of the 700 MHz NMR spectrometer at ISU will enhance ongoing research projects related to human health by providing greater access to high field instrumentation as well as additional capabilities currently not available on the 700 MHz instrument.

爱荷华州州立大学生物分子核磁共振设施正在寻求资金来更换和升级 用于700 MHz核磁共振（NMR）的控制台、冷冻探针和配件 光谱仪该仪器服务于爱荷华州一个充满活力的研究小组 大学（ISU）从事NIH资助的研究项目，解决与以下相关的问题 生物分子结构和复合物，天然产物，蛋白质紊乱/聚集， 代谢组学和小分子药物发现。所要求的升级将大大 增加国际空间大学高场核磁共振光谱仪的使用， 我们教师的目标有待实现。正如ISU的结构生物学家社区 在过去的十年里，生物分子核磁共振设施迅速发展， 对NMR时间的需求增加。具体要求包括更换所有控制台电子设备， 所有磁体附件，如冷冻刀、完整的冷冻探针系统和添加 自动进样器最初的54毫米口径16.5 T布鲁克UltraShield超导磁体将 保持。NMR磁体通常可使用几十年（更长 比consoles）。现有的15岁的控制台已经达到了其使用寿命的终点， 最先进的生物分子核磁共振研究仪器。维修停机时间的需要 近年来，700 MHz仪器稳步增长，同时， 制造商不再保证更换零件的可用性。即使在好的时候 操作条件下，光谱仪的性能不符合标准， 现代方法，特别是核弛豫色散实验所需的 ISU研究人员。因此，成功升级国际空间大学的700兆赫核磁共振波谱仪将有助于 加强正在进行的与人类健康有关的研究项目， 现场仪表以及目前在700 MHz上不可用的附加功能 仪器