Beckman Optima Multiwavelength Analytical Ultracentrifuge

项目摘要/摘要 在宾夕法尼亚州立大学完善的X射线结晶学设施中，我们寻求 配备Beckman Coulter多波长Optima分析超速离心仪(AUC)的资金 同时具有紫外可见吸收和瑞利干涉光学性能。与强大的AUC合作 软件，超声波扫描和SedFIT，这将使准确的大小，形状，部分比体积， 构象变化、摩尔质量、非均质性、结合热力学和化学计量数据 宿主疾病相关的生物分子，细菌，病毒，运动，核小体和本质上的无序 蛋白质、病毒衣壳、核酸、脂质体及其复合体。没有其他功能正常的AUC设备 存在于我们宾夕法尼亚州立大学的24个校区中的任何一个。现代化的AUC功能，将使我们能够满足 称职地满足我们许多特殊的NIH资助的生物物理和结构表征需求 并支持他们进行综合结构生物学研究。我们已经确定了贝克曼AUC Optima作为唯一符合我们要求的商业产品，具有最先进的技术和高性价比。 它具有0.001厘米的径向分辨率，在吸光度模式下扫描速度高达8秒/通道，5 干扰模式下的秒/通道。扫描速度快，分辨率好，灵敏度高 NIH 22个尖端医疗保健项目及其应用的成功至关重要 可进行多波长消光检测，包括(A)蛋白质复合体和低聚物 Dokholyan，Hancock，Weinert，Boehr，Cotruvo，Zhang和Nixon Labs(B)蛋白质核酸复合体 Tan，Krasilnikov，Murakami，Grigoryev和Moustafa实验室(C)中的液-液分离体系 Bevilacqua Lab(D)Showalt和Zhang Labs(E)脂质体中固有的无序蛋白质 Booker、Boal、Cotruvo和Weinert实验室的厌氧金属酶复合体。这个 能够通过室内多角度光散射、小角度来补充溶液状态AUC实验 高分辨X射线结晶学和低温电子显微镜前的X射线散射和等温量热法 学习将是一种额外的奖励。通过这次握手，我们投资于真正的协同效应，实验结果 将指导结构解释和结合热力学。这些设备将是 由我们两个校区--大学公园和好时大学的18名结构生物学教师使用，以及 三所学院，医学院、埃伯利理学院和工程学院。使用一个 最先进的多波长Beckman Optima系统，我们的目标是巧妙地解决超速离心法 数据在光谱和水动力领域有着广泛的应用范围，适用于大量的 并加强宾夕法尼亚州立大学X射线结晶学核心设施的能力。

Project Summary/Abstract At the well-established X-ray crystallography facility at The Pennsylvania State University, we seek funds for a Beckman Coulter multiwavelength Optima analytical ultracentrifuge (AUC) instrument equipped with both UV-visible absorbance and Rayleigh interference optics. Partnered with the robust AUC software, UltraScan and SedFIT, this will enable accurate size, shape, partial specific volume, conformational change, molar mass, heterogeneity, binding thermodynamics and stoichiometry data on a host of disease related biomolecules, bacterial, viral, motor, nucleosomal and intrinsically disordered proteins, viral capsids, nucleic acids, liposomes and their complexes. No other functional AUC equipment exists on any of our twenty-four Penn State campuses. A modern AUC capability, will enable us to cater competently to the biophysical and structural characterization needs of our many exceptional NIH funded faculty and support them in an integrated structural biology research. We have identified the Beckman AUC Optima as the sole commercial product that meets our requirements, is state-of-the-art and cost-effective. It features 0.001 cm radial resolution, scanning speeds up to 8 sec/channel in absorbance mode, and 5 sec/channel in interference mode. The high scan-speed, good resolution and superior sensitivity is paramount for the success of the twenty-two cutting edge health care NIH projects and their applications amenable to multiwavelength extinction detection including (a) protein complexes and oligomers in the Dokholyan, Hancock, Weinert, Boehr, Cotruvo, Zhang and Nixon labs (b) protein nucleic acid complexes in the Tan, Krasilnikov, Murakami, Grigoryev and Moustafa labs (c) liquid-liquid phase separated systems in the Bevilacqua lab (d) intrinsically disordered proteins in the Showalter and Zhang labs (e) liposomes in the Tian lab and (f) anaerobic metalloenzyme complexes in the Booker, Boal, Cotruvo and Weinert labs. The ability to compliment the solution state AUC experiment with in-house multiangle light scattering, small angle X-ray scattering and isothermal calorimetry prior to high resolution X-ray crystallography and cryoEM studies would be a bonus. With this handshake, we are investing in true synergy where experimental results from the AUC would guide the structural interpretation and binding thermodynamics. The equipment will be used by eighteen structural biology faculty from two of our campuses, University Park and Hershey, and three colleges, College of Medicine, Eberly College of Science, and the College of Engineering. With a state-of-the-art multiwavelength Beckman Optima system, our goal is to ably resolve ultracentrifugation data in the spectral and hydrodynamic domains in a wide size range of applications, for a large number of users and enhance the capability at the Penn State X-ray crystallography core facility.