Tissue and neurobehavioral phenotyping core

项目总结 组织和神经行为表型核心，被指定为核心C，是一个集中化的设施，将 为研究人员提供一种成熟的、可复制的甲型流感肺炎模型。 定量工具，如肺力学的Flexivent测量，磁共振成像 评估肺修复和重塑，并将进行促炎症细胞因子的多种措施 根据项目1、2和3中概述的研究计划由核心C进行。核心C将使用高级流程 细胞计数技术能够量化、表型和分类特定的炎性和实质细胞群 取自小鼠的肺、脑和肌肉。核心的分类和分离能力将允许细胞类型- 通过质谱学对蛋白质平衡网络的具体评估(如核心B所概述的)。核心C将 培育小鼠以产生细胞类型或组织特异性的Cre重组酶系以诱导组织特异性 基因敲除老鼠。核心C将对项目负责人提出的所有小鼠品系进行基因分型。堆芯 C将维持一个统一的环境，使野生型和转基因小鼠老化。 重要的是，核心C将评估甲型流感引发的肺炎对 神经行为表型。简而言之，核心C将检查运动的协调和平衡通过 加速旋转棒试验。将测试热板上的反应潜伏期，以评估伤害性功能。 探索性行为和活动活动将在开阔的野外测试中和连续的 用转动的轮子进行监控。学习和记忆将在新奇物体识别和巴恩斯中得到检验 迷宫测试。总的来说，这些工具为项目调查员提供了独特的资源，这将是 如果没有这个PPG的支持，很难复制。

PROJECT SUMMARY The Tissue and Neurobehavioral Phenotyping Core, designated as Core C, is a centralized facility that will provide investigators with a well-established and reproducible model of influenza A–induced pneumonia. Quantitative tools such as Flexivent measurements of lung mechanics, magnetic resonance imaging to evaluate lung repair and remodeling, and multiplex measures of pro-inflammatory cytokines will be conducted by Core C according to the research plan outlined in Projects 1, 2, and 3. Core C will use advanced flow cytometry capabilities to quantify, phenotype, and sort specific inflammatory and parenchymal cell populations from the murine lung, brain, and muscle. The sorting and isolation capabilities of the Core will allow cell-type- specific assessment of proteostasis networks via mass spectroscopy (as outlined by Core B). Core C will breed mice to generate the cell-type- or tissue-specific Cre recombinase lines to induce tissue-specific knockout mice. Core C will perform the genotyping of all the murine strains proposed by Project Leaders. Core C will maintain a uniform environment in which wild-type and genetically engineered mice will be aged. Importantly, Core C will assess the impact of influenza A–induced pneumonia on age-related changes in neurobehavioral phenotypes. Briefly, Core C will examine motor coordination and balance by means of an accelerating rotarod test. Response latency on a hot plate will be tested to assess nociceptive functions. Exploratory behaviors and locomotor activity will be examined in an open field assay and by continuous monitoring with running wheels. Learning and memory will be examined in novel object recognition and Barnes maze tests. Collectively, these tools provide a unique resource for Project Investigators, which would be difficult to reproduce without the support of this PPG.