Tissue and neurobehavioral phenotyping core

组织和神经行为表型核心

• 批准号: 10620765
• 负责人: KAREN M RIDGE
• 金额: $ 29.41万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10620765
• 关键词: Acceleration; Aging; Alveolar; Animal Model; Attention; Biological Assay; Blinded; Brain; Breeding; Cells; Disease; Enterobacteria phage P1 Cre recombinase; Environment; Equilibrium; Exploratory Behavior; Flow Cytometry; Funding Opportunities; Generations; Genes; Genetically Engineered Mouse; Genotype; Human Resources; Immune; Inflammatory; Influenza A virus; Injury; Instruction; Intervention; Knock-out; Knockout Mice; Learning; Liquid substance; LoxP-flanked allele; Lung; Magnetic Resonance Imaging; Mass Spectrum Analysis; Measurement; Measures; Mechanics; Memory; Modeling; Monitor; Motor; Motor Activity; Mus; Muscle; Muscle function; Nociception; Organ; Outcome Measure; Pathway interactions; Phenotype; Pneumonia; Population; Program Research Project Grants; Protocols documentation; Pulmonary Inflammation; Reaction; Reproducibility; Research; Research Personnel; Resources; Response Latencies; Rotarod Performance Test; Running; Skeletal Muscle; Sorting; Standardization; Techniques; Testing; Tissues; Travel; Virus Diseases; age related; aged; behavior test; cell type; cost; cytokine; experience; falls; indexing; influenza A pneumonia; influenza infection; interest; knockout animal; lung injury; lung repair; mouse model; neurobehavioral; novel; object recognition; programs; proteostasis; recruit; synergism; tool; transcriptomics

PROJECT SUMMARY The Tissue and Neurobehavioral Phenotyping Core, designated as Core C, is a centralized facility that will provide investigators with a well-established and reproducible model of influenza A–induced pneumonia. Quantitative tools such as Flexivent measurements of lung mechanics, magnetic resonance imaging to evaluate lung repair and remodeling, and multiplex measures of pro-inflammatory cytokines will be conducted by Core C according to the research plan outlined in Projects 1, 2, and 3. Core C will use advanced flow cytometry capabilities to quantify, phenotype, and sort specific inflammatory and parenchymal cell populations from the murine lung, brain, and muscle. The sorting and isolation capabilities of the Core will allow cell-type- specific assessment of proteostasis networks via mass spectroscopy (as outlined by Core B). Core C will breed mice to generate the cell-type- or tissue-specific Cre recombinase lines to induce tissue-specific knockout mice. Core C will perform the genotyping of all the murine strains proposed by Project Leaders. Core C will maintain a uniform environment in which wild-type and genetically engineered mice will be aged. Importantly, Core C will assess the impact of influenza A–induced pneumonia on age-related changes in neurobehavioral phenotypes. Briefly, Core C will examine motor coordination and balance by means of an accelerating rotarod test. Response latency on a hot plate will be tested to assess nociceptive functions. Exploratory behaviors and locomotor activity will be examined in an open field assay and by continuous monitoring with running wheels. Learning and memory will be examined in novel object recognition and Barnes maze tests. Collectively, these tools provide a unique resource for Project Investigators, which would be difficult to reproduce without the support of this PPG.

项目概要 组织和神经行为表型核心，指定为核心 C，是一个集中设施，将 为研究人员提供了一个完善且可重复的甲型流感诱发的肺炎模型。 定量工具，例如肺力学的 Flexivent 测量、磁共振成像 评估肺修复和重塑，并对促炎细胞因子进行多重测量 由Core C根据项目1、2和3中概述的研究计划进行。Core C将使用先进的流程 细胞计数功能可对特定炎症和实质细胞群进行量化、表型分析和分类 来自小鼠肺、大脑和肌肉。核心的分选和分离能力将允许细胞类型 通过质谱法对蛋白质稳态网络进行具体评估（如核心 B 所述）。核心C将 培育小鼠以产生细胞类型或组织特异性的 Cre 重组酶系，从而诱导组织特异性 基因敲除小鼠。核心 C 将对项目负责人提出的所有小鼠品系进行基因分型。核 C将维持野生型和基因工程小鼠衰老的统一环境。 重要的是，核心 C 将评估甲型流感诱发的肺炎对年龄相关变化的影响 神经行为表型。简而言之，核心 C 将通过 加速转棒试验。将测试热板上的反应延迟以评估伤害感受功能。 探索行为和运动活动将通过旷场测定和连续测试进行检查 用跑轮进行监控。学习和记忆将在新物体识别和巴恩斯研究中得到检验 迷宫测试。总的来说，这些工具为项目调查人员提供了独特的资源，这将是 如果没有这个 PPG 的支持，很难重现。