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Extracellular vesicles as biomarkers of trauma exposure and PTSD risk

细胞外囊泡作为创伤暴露和 PTSD 风险的生物标志物

基本信息

批准号：
10420911
负责人：
Tracy L Bale
金额：
$ 76.9万
依托单位：
UNIVERSITY OF COLORADO DENVER
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-01 至 2027-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10420911
关键词：
17q21 Acute Adolescence Adolescent Adult Age Behavior Biological Biological Markers Birth Black race Brain Cations Characteristics Childhood Chronic Collection Complex Data Development Discrimination Disease Outcome Exposure to Freezing Fright Galvanic Skin Response Gene Cluster Health Human Individual Interpersonal Violence Intervention Keratin Light Link Longevity Longitudinal cohort Measures Mental Health Merkel Cells Molecular Mus Neurites Neuroendocrine Cell Neurons Outcome Pathway interactions Physiological Piezo 2 ion channel Play Population Post-Traumatic Stress Disorders Production Proteins Proteomics Psychophysiology Reporting Research Domain Criteria Resources RiboTag Risk Role Sensory Sensory Thresholds Skin Startle Reaction Stimulus Stress Tactile Testing Tissues Touch sensation Trauma Woman age group base black men black women cohort conditioned fear designer receptors exclusively activated by designer drugs disorder risk emotional abuse experience extracellular vesicles insight keratinocyte men mouse model multimodality nanoparticle neurophysiology novel novel marker potential biomarker programs prospective response specific biomarkers tactile stimulation transcriptomics translational study trauma exposure violence exposure young adult zeta potential

项目摘要

Exposure to interpersonal violence (IPV) is associated with long-term negative mental health consequences, including an increased risk for posttraumatic stress disorder (PTSD), especially in women. The specific developmental timing of IPV is likely to play a significant role in disease outcomes where trauma experienced in late adolescence increases PTSD risk by 3-fold. However, little is known as to timing effects of IPV and PTSD risk related to sensitive periods of brain development and maturation. Our previous studies examined IPV experienced during specific developmental windows on health outcomes in a predominantly Black cohort of adult women. Our results revealed that psychophysiological reactivity related to PTSD risk, skin conductance response (SCR) and fear-potentiated startle, was uniquely observed when IPV was experienced between 14-18 yrs. Using an unbiased proteomic approach in extracellular vesicles (EV) to identify potential biomarkers specific to timing of IPV experience, we found a unique EV protein signature belonging to a 17q21 gene cluster and associated with skin keratinocytes, specifically unique to Merkel cells. Merkel cells are mechanosensitive neuroendocrine cells in the skin innervated by sensory Ab neurons that detect light touch stimuli via Piezo2 cation channels. We found similar changes in a mouse model in which multimodal sensory stress was experienced during the pubertal period, including EV proteins associated with the mouse keratin I gene cluster 11qD, and increased adult fear-potentiated startle responses and freezing behaviors. Taken together, these results support tactile-based trauma exposure during late adolescence may increase sensitivity of threat circuitry: the proposed cross-species translational study will examine molecular and physiological levels of analyses of the RDoC Acute Threat construct. In this proposal, we have the unique opportunity to investigate a 30-yr prospective longitudinal Black cohort who have been followed since birth, including collection of IPV data during late adolescence, and to capture their transition through young adulthood, providing insight into the mechanisms and biomarkers related to PTSD risk. Our overarching hypothesis to be tested is that IPV occurring during a sensitive period of late adolescence specifically programs distinct biological pathways along the threat-response axis involving the skin Merkel cell-neurite complex, and that these changes are detectable as accessible biomarkers to be tested in the following Aims: 1) To establish that a sensitive period of late adolescence for IPV experience uniquely associates with psychophysiological measures of acute threat, including SCR, fear- potentiated startle, and quantitative sensory testing in a prospective longitudinal birth cohort of Black men and women; 2) To identify biomarkers from examination of EV proteomics and characteristics relevant to PTSD risk from a prospective longitudinal cohort; and 3) To identify the Merkel cell involvement in production of EVs and alteration of behaviors relevant to PTSD risk using pubertal multimodal sensory stress in a mouse.

暴露于人际暴力（IPV）与长期的负面心理健康后果有关，